长春新碱超顺磁性氧化铁纳米粒的制备及体外评价
Preparation and in vitro evaluation of vincristine-loaded superparamagnetic iron oxide nanoparticles
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摘要: 采用自组装法制备长春新碱超顺磁性氧化铁纳米粒，分别通过邻二氮菲显色法和高效液相色谱法测定制剂中铁浓度和药物浓度，计算包封率；分别用透射电镜、动态光散射法对制得纳米粒的形态、粒径进行了表征；通过振动样品磁强计测定制剂的饱和磁化强度，绘制磁滞回线；以硫酸长春新碱为对照，考察等剂量的长春新碱超顺磁性氧化铁纳米粒对人白血病K562细胞的抑制效果，通过四唑单钠盐法检测细胞活力。结果表明，制得的长春新碱超顺磁性氧化铁纳米粒包封率为(81.2±1.5)%，铁浓度为(104±1.4)μg/mL；磁性纳米粒子呈球形，平均粒径为(83±1.2) nm；饱和磁化强度为53 A·m2/kg，且具有超顺磁性；细胞实验表明长春新碱超顺磁性氧化铁纳米粒比硫酸长春新碱对K562的细胞的抑制作用更明显。结果显示，制得的超顺磁性氧化铁纳米粒性能良好，具有一定的应用前景。Abstract: Vincristine loaded superparamagnetic iron oxide (VCR-SPIO) nanoparticles were prepared by self-assembly process.Phenanthroline spectrophotometry and HPLC were used respectively to determine the concentration of iron and vincristine of the pharmaceutical preparation.Encapsulation efficiency was calculated.The morphology and size of the preparation were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS).Vibrating sample magnetometer (VSM) was used to detect saturation magnetization,and magnetization curve was drawn.Human leukemia K562 cells were cultured in the presence of vincristine sulfate and VCR-SPIO nanoparticles.Viability of cells was determined by CCK-8.It was found that encapsulation efficiency was (81.2±1.5) %.The concentration of iron was (104±1.4)μg/mL.The average particle diameter of the preparation was (83±1.2) nm.VCR-SPIO nanoparticles had superparamagnetic effect with the saturation magnetization of 56.3 A·m2/kg.VCR-SPIO nanoparticles showed higher cytotoxicity on K562 cells.It was shown in this study that the novel multifunctional superparamagnetic iron oxide nanoparticles as a novel drug delivery system had the potential for cancer therapy.
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