HPLC-DPPH在线联用技术评价茶的抗氧化活性

基金项目: 国家自然科学基金资助项目(No.30973965)

Determination of antioxidant activity of tea by HPLC-DPPH

摘要: 基于“谱效整合指纹图谱”的研究思路，构建了HPLC-DPPH在线联用技术，并将该技术应用于茶的抗氧化活性分析。将获得的11批不同品牌茶样品的化学指纹峰（谱）和活性指纹峰（效）的数据进行统计分析处理，换算出11批茶样品的总抗氧化活性。结果显示，不同品牌茶的总抗氧化活性差异较大。该方法可以对茶等保健品中的相关活性物质进行鉴别和定量检测，可以作为对保健品及中药进行质量控制的参考方法。
- 谱效整合指纹图谱 /
- HPLC-DPPH /
- 茶 /
- 在线分析 /
- 抗氧化活性
Abstract: Based on the concept of the activity integrated fingerprint,an on-line HPLC-DPPH system was developed to determine the antioxidant activity of tea.The chromatographic and biological activity fingerprint of tea-scavenging DPPH were simultaneously identified for different brands by HPLC-DPPH.The total antioxidant activities of 11 batches of tea samples were acquired by data analysis,which showed significant differences.The results suggested that the on-line method might reveal the potential information for the identification and quantitation of the active ingredients,which were directly associated with the quality of health foods and herbal medicines.
- activity integrated fingerprint /
- HPLC-DPPH /
- tea /
- on-line analysis /
- antioxidant