固相萃取-荧光分光光度法快速测定脂质体包封率

陈蓉; 于杰; 叶超; 李臣贵; 胡育筑

固相萃取-荧光分光光度法快速测定脂质体包封率

Rapid determination of entrapment efficiency of liposomes by solid phase extraction-fluorospectrophotometry

摘要

摘要: 以紫杉醇作为模型药物，旨在建立一种固相萃取-荧光分光光度法快速测定脂质体包封率的方法。分别采用水和75%甲醇对SampliQ C₁₈固相萃取柱上的紫杉醇脂质体及其游离态进行洗脱分离，并在激发波长λ_ex=244 nm、发射波长λ_em=311 nm条件下，利用荧光分光光度法测定游离紫杉醇的含量，从而间接计算紫杉醇脂质体的包封率。在本文建立的方法下，紫杉醇在0.006～0.078 mg/mL范围内线性关系良好（r=0.998），C₁₈固相萃取柱对空白脂质体和游离紫杉醇的回收率均在97.5%以上。该方法测得自制紫杉醇脂质体包封率为50.8%（RAD=1.5%），与微柱离心法测得结果相比无显著性差异。实验结果表明，本方法可快速、简便地实现游离紫杉醇与其脂质体的分离测定，为脂质体的质量控制研究提供参考。

Abstract: The objective of this study was to establish a method for the determination of the entrapment efficiency of liposomes by solid phase extraction-fluorospectrophotometry.Paclitaxel was selected as the model drug.SampliQ C₁₈ solid phase extraction columns were applied to separate free paclitaxel and its liposome using water and 75% methanol solution as elute solutions,respectively.A fluorospectrophotometries method (λ_ex=244 nm,λ_em=311 nm) was applied to determine the entrapment efficiency of paclitaxel liposome.The calibration curve was linear in the range of 0.006-0.078 mg/mL(r=0.998),and the recoveries of blank liposome and free paclitaxel were all above 97.5%.The entrapment efficiency of paclitaxel liposome determined by this method was 50.8%（RAD=1.5%）,which has no significant difference from those results measured by mini-column centrifugation.Solid phase extraction-fluorospectrophotometry could be applied to determine the entrapment efficiency of paclitaxel liposome rapidly and accurately and could be used to control the quality of paclitaxel liposome.

HTML全文

参考文献(0)

施引文献

资源附件(0)