亲水作用色谱在体内药物定量分析中的应用进展

Hydrophilic interaction chromatography and its application in quantitative bioanalysis

摘要: 亲水作用色谱（HILIC）是一种结合了亲水性固定相与极性有机溶剂-水体系流动相的色谱分离技术，对反相色谱中难以保留的极性化合物能够实现有效分离。近年来，将HILIC-串联质谱联用技术（HILIC-MS/MS）应用于体内药物定量分析的报道已引起广泛关注。本文简要介绍了HILIC的定义和机制，并综述了其在体内药物定量分析中的应用进展。
- 亲水作用色谱 /
- 体内药物分析 /
- 极性化合物 /
- 定量分析
Abstract: Hydrophilic interaction chromatography(HILIC),combining polar organic-aqueous mobile phase with a hydrophilic stationary phase,has been shown to be a powerful tool for separating polar analytes which have poor retention on traditional reversed-phase liquid chromatography(RPLC). HILIC-tandem mass spectrometry (HILIC-MS/MS) has attracted growing interest in the quantitation of analytes from complex biological matrices. This article briefly reviews the concept and mechanism of HILIC system,and its recent applications in quantitative bioanalysis.
- hydrophilic interaction liquid chromatography /
- bioanalysis /
- polar analytes /
- quantitative analysis