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亲水作用色谱在体内药物定量分析中的应用进展

Hydrophilic interaction chromatography and its application in quantitative bioanalysis

  • 摘要: 亲水作用色谱(HILIC)是一种结合了亲水性固定相与极性有机溶剂-水体系流动相的色谱分离技术,对反相色谱中难以保留的极性化合物能够实现有效分离。近年来,将HILIC-串联质谱联用技术(HILIC-MS/MS)应用于体内药物定量分析的报道已引起广泛关注。本文简要介绍了HILIC的定义和机制,并综述了其在体内药物定量分析中的应用进展。

     

    Abstract: Hydrophilic interaction chromatography(HILIC),combining polar organic-aqueous mobile phase with a hydrophilic stationary phase,has been shown to be a powerful tool for separating polar analytes which have poor retention on traditional reversed-phase liquid chromatography(RPLC). HILIC-tandem mass spectrometry (HILIC-MS/MS) has attracted growing interest in the quantitation of analytes from complex biological matrices. This article briefly reviews the concept and mechanism of HILIC system,and its recent applications in quantitative bioanalysis.

     

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