• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WU Lili, ZHOU Ke, XU Lanfang, CHEN Yun, SHENG Chunquan. Tape stripping technique in combination with LC-MS method for the determination of iodiconazole in human stratum corneum[J]. Journal of China Pharmaceutical University, 2014, 45(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20140114
Citation: WU Lili, ZHOU Ke, XU Lanfang, CHEN Yun, SHENG Chunquan. Tape stripping technique in combination with LC-MS method for the determination of iodiconazole in human stratum corneum[J]. Journal of China Pharmaceutical University, 2014, 45(1): 79-83. DOI: 10.11665/j.issn.1000-5048.20140114
shu

Tape stripping technique in combination with LC-MS method for the determination of iodiconazole in human stratum corneum

More Information
    Graphical Abstract
    • Abstract
  • A quantitative analysis method of iodiconazole concentrations in human stratum corneum was established. Human superficial skin samples were obtained by tape stripping technique and iodiconazole concentrations were determined by LC-MS method after extraction. Ketoconazole was used as the internal standard(IS). The analytes were chromatographically separated on a Shim-pack VP-ODS C18 column with the mobile phase consisting of methanol and 0. 01% formic acid aqueous solution(65 ∶35). Mass spectrometer was operated in positive ion and selective ion monitoring(SIM)mode using electrospray ionization(ESI)technique. The method showed excellent linearity over the concentration range of 0. 6-1 800 ng/mL. The recovery and the intra- and inter-batch precisions met the requirements for the determination of biological samples. The dynamic process of intradermal drug concentrations in 10 healthy volunteers after topical application of iodiconazole cream was investigated using this method. The method proved to be selective and sensitive, and suitable for the determination of iodiconazole concentration in human stratum corneum and the pharmacokinetic study after topical application of iodiconazole cream.
    • FullText(HTML)
    • References (11)
  • [1]
    Sheng CQ, Zhang WN, Ji HT, et al. Synthesis and antifungal activity of 1-(1H-1,2,4-triazole)-2-(2,4-diflurophenyl)-3-(N-methyl-N-substitutedbenzylamino)-2-propanols[J].Chin Pharm J(中国药学杂志),2002,11(2):5-10.
    [2]
    Sheng CQ. Computer-aided design and synthesis of novel antifungal agents(计算机辅助新型抗真菌药物设计与合成)[D].Shanghai: Second Military Medical University,2005.
    [3]
    Zhang WN, Ji HT, Zhou YJ, et al. Synthesis of triazole alcohol antifungal compounds(三氮唑醇类抗真菌化合物及其制备方法): CN,1292378A[P].2001-04-25[2013-09-24].
    [4]
    Sheng CQ,Zhang WN,Ji HT,et al.Innovative design of novel antifungal agents[J].Chin New Drugs J(中国新药杂志),2004,13(2):97-102.
    [5]
    Herkenne C,Alberti I,Naik A,et al.In vivo methods for the assessment of topical drug bioavailability[J].Pharm Res,2008,25(1):87-103.
    [6]
    Narkar Y.Bioequivalence for topical products—an update[J].Pharm Res,2010,27(12):2 590-2 601.
    [7]
    Lionberger RA. FDA critical path initiatives: opportunities for generic drug development[J].AAPS J,2008,10(1):103-109.
    [8]
    Xie SM.Pay attention to dermatopharmacokinetics in investigation on the topical dermatological drugs[J].Chin J Clin Pharmacol(中国临床药理学杂志),2007,23(4):313-315.
    [9]
    Wen J,Fan GR,Hong ZY,et al.High performance liquid chromatographic determination of a new antifungal compound,ADKZ in rat plasma[J].J Pharm Biomed Anal,2007,43(2):655-658.
    [10]
    Gao SH,Tao X,Sun LN,et al.An liquid chromatography-tandem mass spectrometry assay for determination of trace amount of new antifungal drug iodiconazole in human plasma[J].J Chromatogr B Analyt Technol Biomed Life Sci,2009,877(4):382-386.
    [11]
    Sun N, Wen J, Lu GC, et al. An ultra-fast LC method for the determination of iodiconazole in microdialysis samples and its application in the calibration of laboratory-made linear probes[J].J Pharm Biomed Anal,2010,51(1):248-251.
    • Related Articles

  • Related Articles

    [1]SHI Min, YONG Qin, HE Yingna, HUANG Shiqin, ZHAO Xuan, YU Xian. Construction and in vivo evaluation of a thermosensitive hydrogel system loading with Pseudomonas aeruginosa DNA vaccine[J]. Journal of China Pharmaceutical University, 2021, 52(2): 186-194. DOI: 10.11665/j.issn.1000-5048.20210207
    [2]CHEN Ye, YIN Jun, YAO Wenbing, GAO Xiangdong. Advances of DNA-based nanomaterials in tumor therapy[J]. Journal of China Pharmaceutical University, 2020, 51(4): 406-417. DOI: 10.11665/j.issn.1000-5048.20200404
    [3]WANG Rui, WANG Yongmei, CAI Mingjun, KE Xuejia, WU Yue, CHONG Jun, CAO Rongyue. Pharmacological effects of anti-melanoma DC vaccine sensitized by fusion proteins of G3G6 and HST1[J]. Journal of China Pharmaceutical University, 2019, 50(2): 238-245. DOI: 10.11665/j.issn.1000-5048.20190216
    [4]HAN Xiu, QI Xiaole, WU Zhenghong. Advances in self-assemblied DNA nanocages as drug delivery systems[J]. Journal of China Pharmaceutical University, 2017, 48(6): 663-669. DOI: 10.11665/j.issn.1000-5048.20170605
    [5]ZHANG Zhiqing, WANG Fang, ZHOU Ting, ZHANG Guodong, WANG Xiufeng, LI Yunze. A DNA polyaptamer system as a targeted antitumor drug delivery[J]. Journal of China Pharmaceutical University, 2015, 46(4). DOI: 10.11665/j.issn.1000-5048.20150408
    [6]Formula optimization and transfection efficiency of polyethyleneimine/DNA complexes[J]. Journal of China Pharmaceutical University, 2010, 41(1): 40-44.
    [7]Study on the Flavonoids from Lonicera confusa DC.[J]. Journal of China Pharmaceutical University, 2004, (4): 9-12.
    [8]Applications of Au Nanoparticles Labeling Method in DNA-detection and the Technology of Genechip[J]. Journal of China Pharmaceutical University, 2003, (2): 88-93.
    [9]The Isoquinolones from Menispermum dauricum DC.[J]. Journal of China Pharmaceutical University, 2001, (2): 16-17.
    [10]Ptotoplast Transformation of Staphylococcal DNA[J]. Journal of China Pharmaceutical University, 1992, (4): 239-242.

Catalog

    Get Citation
    PDF
    XML
    Article views (1078) PDF downloads (1973) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：309717

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return