Interaction of oleanolic acid and its derivatives with bovine serum albumin by spectrofluorimetry</b>

ZHANG Tianxing; HUANG Wei; HUANG Xueyu; LIAO Anping

doi:10.11665/j.issn.1000-5048.20170511

Journal of China Pharmaceutical University > 2017 > 48(5): 572-576. > DOI: 10.11665/j.issn.1000-5048.20170511

ZHANG Tianxing, HUANG Wei, HUANG Xueyu, LIAO Anping. Interaction of oleanolic acid and its derivatives with bovine serum albumin by spectrofluorimetry[J]. Journal of China Pharmaceutical University, 2017, 48(5): 572-576. DOI: 10.11665/j.issn.1000-5048.20170511

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Interaction of oleanolic acid and its derivatives with bovine serum albumin by spectrofluorimetry

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Oleanolic acid was chemically modified to improve its interaction with bovine serum albumin, thus to increase the mutual binding ability. As a lead compound, oleanolic acid reacted with Jones-reagent, then methyl iodide and finally hydroxylamine to obtain four different derivatives. Their structures were confirmed by IR and ¹H NMR analysis. The interaction between these derivatives and bovine serum albumin and the effect of temperature and trace metal ions as Cu²⁺ and Co²⁺ on the interaction were investigated by fluorescence spectroscopy. All these derivatives exhibited improvement of the interaction compared with oleanolic acid with 16 times stronger for OA₂. The interaction also increased with the presence of trace metal ions as Cu²⁺and Co²⁺. These results indicate that chemical modification can improve the interaction of oleanolic acid with bovine serum albumin.
- oleanolic acid,
- derivatives,
- bovine serum albumin,
- interaction,
- spectrofluorimetry

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