• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WANG Shuo, SUN Xiaoyan, CHEN Jinlong. Application of rhodamine-based fluorescent molecular probes in visualization of cellular pyruvic acid[J]. Journal of China Pharmaceutical University, 2018, 49(1): 79-86. DOI: 10.11665/j.issn.1000-5048.20180111
Citation: WANG Shuo, SUN Xiaoyan, CHEN Jinlong. Application of rhodamine-based fluorescent molecular probes in visualization of cellular pyruvic acid[J]. Journal of China Pharmaceutical University, 2018, 49(1): 79-86. DOI: 10.11665/j.issn.1000-5048.20180111
shu

Application of rhodamine-based fluorescent molecular probes in visualization of cellular pyruvic acid

More Information
    Graphical Abstract
    • Abstract
  • In order to conduct the visualized research of cellular pyruvic acid(PA), several rhodamine-based probes were designed to react with PA by a Schiff-base reaction in aqueous media and exhibit strong fluorescence emission and a pink color owing to the ring-opening of the corresponding spirolactam. The results showed that R6G-NH2 had a highest sensitive response to PA with a linearly relationship between the enhancement of fluorescence intensity at 565 nm and the concentration of PA from 0. 2 μmol/L to 120 μmol/L. According to the principle of 3σ, a limit of detection was obtained as low as 0. 1 μmol/L. Furthermore, the response of R6G-NH2 to PA was hardly affected by conventional biomolecules and ionic species. Therefore, R6G-NH2 was successfully used in fluorescence imaging of exogenous PA in non-small cell lung cancer cell A549 and renal epithelial cell 293T, and endogenous PA induced by bisphenol A in epithelial cell 293T.
    • FullText(HTML)
    • References (24)
  • [1]
    Li Q,Li S,Chen X,et al.A G-quadruplex based fluorescent oligonucleotide turn-on probe towards iodides detection in real samples[J].Food Chem,2017,230(1):432-440.
    [2]
    Peng T,Chen XM,Gao L,et al.A rationally designed rhodamine-based fluorescent probe for molecular imaging of peroxynitrite in live cells and tissues[J].Chem Sci,2016,7(8):5407-5413.
    [3]
    Ma QJ,Zhang XB,Zhao XH,et al.A highly selective fluorescent probe for Hg2+ based on a rhodamine-coumarin conjugate[J].Anal Chim Acta,2010,663(1):85-90.
    [4]
    Li K,Xiang Y,Wang XY,et al.Reversible photochromic system based on rhodamine B salicylaldehyde hydrazone metal complex[J].J Am Chem Soc,2014,136(4):1643-1649.
    [5]
    Wu YX,Li JB,Liang LH,et al.A rhodamine-appended water-soluble conjugated polymer:an efficient ratiometric fluorescence sensing platform for intracellular metal-ion probing[J].Chem Commun(Camb),2014,50(16):2040-2042.
    [6]
    Zhou LY,Zhang XB,Wang QQ,et al.Molecular engineering of a TBET-based two-photon fluorescent probe for ratiometric imaging of living cells and tissues[J].J Am Chem Soc,2014,136(28):9838-9841.
    [7]
    Gao H,Song J,Shang S,et al.Fluorescent properties and in vitro studies of new dehydroabietic acid-based diarylamines fluorescent probes[J].Front Agr Sci Eng,2017,4(1):106-115.
    [8]
    Liu H,Na W,Liu Z,et al.A novel turn-on fluorescent strategy for sensing ascorbic acid using graphene quantum dots as fluorescent probe[J].Biosens Bioelectron,2017,92(15):229-233.
    [9]
    Madrakian T, Maleki S, Gilak S, et al. Turn-off fluorescence of amino-functionalized carbon quantum dots as effective fluorescent probes for determination of isotretinoin[J].Sens Actuators B Chem,2017,427:428-435.
    [10]
    Collie JTB,Greaves RF,Jones OAH,et al.Vitamin B1 in critically ill patients:needs and challenges[J].Clin Chem Lab Med,2017,55(11),1652-1668.
    [11]
    Wu WT.Biochemistry(生物化学)[M].3rd Ed.Beijing:China Medical Science and Technology Press,2015:351-365.
    [12]
    Olsen C.An enzymatic fluorimetric micromethod for the determination of acetoacetate,β-hydroxybutyrate,pyruvate and lactate[J].Clin Chim Acta,1971,33(2):293-300.
    [13]
    Horowitz A,Meller R,Moortgat GK.The UV-VIS absorption cross sections of the α-dicarbonyl compounds:pyruvic acid,biacetyl and glyoxal[J].J Photochem Photobiol A Chem,2001,146(1):19-27.
    [14]
    Pérez-Ruiz T,MartíNez-Lozano C,Tomás V,et al.Chemiluminescence determination of citrate and pyruvate and their mixtures by the stopped-flow mixing technique[J].Anal Chim Acta,2003,485(1):63-72.
    [15]
    Ewaschuk JB,Naylor JM,Barabash WA,et al.High-performance liquid chromatographic assay of lactic,pyruvic and acetic acids and lactic acid stereoisomers in calf feces,rumen fluid and urine[J].J Chromatogr B,2004,805(2):347-351.
    [16]
    Hautala E,Weaver ML.Separation and quantitative determination of lactic,pyruvic,fumaric,succinic,malic,and citric acids by gas chromatography[J].Anal Biochem,1969,30(1):32-39.
    [17]
    Li L,Li GX,Dai QW.Analysis of the content of phenylpyruvic acid in mixture by colorimetry[J].Chem Reagents(化学试剂),2002,24(1):22-23.
    [18]
    Lou LW,Gao NF.Determination of pyruvate by ultraviolet spectrophotometry[J].Anal Lab(分析试验室),2005,24(4):11-13.
    [19]
    Zhang Z,Deng CQ,Guo T,et al.Synthesis and analytical application of a rhodamine-based fluorescent probe for hypochlorous acid[J].J Instrum Anal(分析测试学报),2014,33(7):810-814.
    [20]
    Zhou L,Liu W,Di B,et al.Synthesis and application of a fluorescent molecular probe for rapid detection of sulfur dioxide residues in traditional Chinese herbs[J].J China Pharm Univ(中国药科大学学报),2015,46(4):444-449.
    [21]
    Jiao CH,He Y,Jin PW,et al.A turn-on fluorescent probe for α-ketoglutaric acid based on rhodamine B[J].Imag Sci Photochem(影像科学与光化学),2015,33(3):195-202.
    [22]
    Wang JT.Organic Chemistry(有机化学)[M].2nd Ed.Tianjin:Nankai University Press,2009:348-350.
    [23]
    Guo AG.Basic Biochemistry(基础生物化学)[M].2nd Ed.Beijing:Higher Education Press,2013:247-254.
    [24]
    Shi XY,Wang Z,Liu L,et al.Low concentrations of bisphenol a promote human ovarian cancer cell proliferation and glycolysis-based metabolism through the estrogen receptor-α pathway[J].Chemosphere,2017,185:361-367.
    • Related Articles

  • Related Articles

    [1]XIE Jing, FAN Chunlin, XU Jie, ZHANG Jian, YE Wencai, ZHANG Xiaoqi. Alkaloids of Ervatamia pandacaqui[J]. Journal of China Pharmaceutical University, 2021, 52(3): 287-292. DOI: 10.11665/j.issn.1000-5048.20210304
    [2]LI Linzhen, WEI Xi, LIU Lu, LI Yongjun, LIANG Jingyu. Chemical constituents from the stems of Clerodendrum trichotomum Thunb.[J]. Journal of China Pharmaceutical University, 2019, 50(5): 544-548. DOI: 10.11665/j.issn.1000-5048.20190506
    [3]LIN Qinghua, XU Jian, FENG Feng. Chemical constituents from the stems of Picrasma quassioides Bennet[J]. Journal of China Pharmaceutical University, 2017, 48(6): 675-679. DOI: 10.11665/j.issn.1000-5048.20170607
    [4]HUANG Qilong, ZHANG Wanjin, LI Yan, CHEN Juan, ZHOU Baoping, ZOU Xiaohan, ZHANG Chunlei, CAO Zhengyu. Alkaloid constituents from Corydalis decumbens[J]. Journal of China Pharmaceutical University, 2017, 48(5): 563-567. DOI: 10.11665/j.issn.1000-5048.20170509
    [5]XU Yunhui, JIANG Xueyang, XU Jian, JIANG Renwang, ZHANG Jie, XIE Zijian, FENG Feng. Chemical constituents from Callicarpa kwangtungensis Chun[J]. Journal of China Pharmaceutical University, 2016, 47(3): 299-302. DOI: 10.11665/j.issn.1000-5048.20160309
    [6]MA Lin, ZHANG Rongfei, YU Shule, WU Zhengfeng, ZHAO Shouxun, Wang Lei, YE Wencai, ZHANG Jian, YIN Zhiqi. Chemical constituents of Fructus Gleditsiae Abnormalis[J]. Journal of China Pharmaceutical University, 2015, 46(2): 188-193. DOI: 10.11665/j.issn.1000-5048.20150209
    [7]LI Linzhen, WANG Menghua, SUN Jianbo, LIANG Jingyu. Chemical constituents from Aletris spicata[J]. Journal of China Pharmaceutical University, 2014, 45(2): 175-177. DOI: 10.11665/j.issn.1000-5048.20140208
    [8]CHANG Bo, XIAO Linjing, ZHANG Jian, ZHAO Shouxun, YE Wencai, YIN Zhiqi. Chemical constituents from Abies ernestii var.salouenensis[J]. Journal of China Pharmaceutical University, 2014, 45(1): 43-47. DOI: 10.11665/j.issn.1000-5048.20140107
    [9]LI Jiu-hui, CHEN Guang-ying, HAN Chang-ri, MO Zheng-rong, SONG Xiao-ping. Chemical constituents from the stems of Vatica mangachpoi Blanco[J]. Journal of China Pharmaceutical University, 2012, 43(1): 25-27.
    [10]Chemical constituents from Senecio nemorensis.[J]. Journal of China Pharmaceutical University, 2010, 41(1): 26-28.

Catalog

    Get Citation
    PDF
    XML
    Article views (929) PDF downloads (1583) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：311768

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return