Quality analysis of 3 species of Radix Paeoniae Alba cultivated in Bozhou by HPLC and LC-MS

t Aim ：To establish the HPLC and LC-MC Methods for the quality analysis of the different species of Radix Paeoniae Alba cultivated in Bozhou. Methods ：The LC-MS method was applied to analyze the chemical composition of the cultivated species，the HPLC method was applied to determine the contents of paeoniforin，benzoic acid and paeonivayin in crude drugs and processing products. Results ：10 characteristic peaks were identified by LC-MS with reference standards.The significant difference was observed in the chemical compositions of 3 cultivated species.The significant difference was observed in the chemical compositions of 3 cultivated species.The content of paeoniflorin was increased significantly after traditional processing.The Results indicated the 4-year-old “pubang” and 5-year-old “xiantiao” are the best cultivated species. Conclusion ：The Methods are simple，rapid and precise to assess and control the quality of different species of Radix Paeoniae Alba cultivated in Bozhou.