Effects of bioactive extracts from Fructus Jujubae in attenuating the inflammation induced by Radix Kansui

Aim ：To evaluate the effects of bioactive extracts from Fructus Jujubae in attenuating the inflammation induced by Radix Kansui. Methods ：Exoteric splenic lymphocyte(SPL) of mouse and peritoneal macrophage(PMφ) of rat were used as cell model to evaluate the anti-inflammatory effects of fractions from Fructus Jujubae.MTT method was used to assay SPL proliferation，and Griess method was used in NO release of PMφ.Firstly， Radix Kansui fraction was applied to induce inflammatory effects of the cells，and then，the anti-inflammatory effects of five extracts of Fructus Jujubae were investigated to determine the active fractions in Fructus Jujubae，which probably contribute to the attenuation of Radix Kansui.The effects of Fructus Jujubae extract in interfering the gastrointestinal acute damages caused by the extract of Radix Kansui were observed according to the pathological sections of mice. Results ：The extract RK-3(diterpenes) exhibited the most significant pro-inflammatory effect.The bioactive extracts B(micromolecule saccharides and amino acids)，D(flavonoid glycosides)，and E(triterpenes) of Fructus Jujubae could attenuate the action of RK-3.The effective extract of Fructus Jujubae protected stomach and duodenum of mouse from acute damages caused by the extract of Radix Kansui. Conclusion ：Fraction RK-3(diterpenes) of Radix Kansui had pro-inflammatory effects，and some extracts of Fructus Jujubae could attenuate this effect of Radix Kansui.The results helped to clarify that Fructus Jujubae attenuates the toxic effect of Radix Kansui in certain cases，and the bioactive extracts of Fructus Jujubae discovered in the experiments offer the basis for further research to the active components of Fructus Jujubae.