Determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum

Aim ：To establish an HPLC method for the determination of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum. Methods ：A column of Shimadzu ODS-C₁₈ column(250 mm×4.6 mm，₅ μm)， and a mobile phase of acetonitrile-water-phosphoric acid(9∶91∶0.1) were adopted.A detect wavelength of 283 nm，a flow rate of 1 mL/min，and a column temperature of 35 °C were set. Results ：The content of 3-methyl-7，8-dihydroxy-isochroman-4-one in the peel of Musa sapientum was 0.029 5%-0.036 7%.Calibration curve was linear over the range 1.09-13.08 μg.The average recovery was 100.0%.The assay variability value was 0.64 %(n=9). Conclusion ：The method can be used for the quality control of Musa sapientum.