An enzyme-linked immunosorbent assay for the determination of sarsasapogenin in Rhizoma Anemarrhenae

Using polyclonal antibody against sarsasapogenin，an enzyme-linked immunosorbent assay (ELISA) for quantitative analysis of sarsasapogenin in Rhizoma Anemarrhenae was developed.The linear detection range of sarsasapogenin was 20-1 311 ng/mL.The lowest limit of detection(LOD) of the assay was 20 ng/mL，and the recovery rate ranged from 92.2% to 97.1%.The relative standard deviation for intra- and inter- assays were less than 14.3%.To validate the immunoassay，the sarsasapogenin content of Rhizoma Anemarrhenae from 5 different habitats were analyzed by the immunoassay and HPLC-ELSD.The results obtained by the two methods were consistent.This immunoassay is simple，sensitive and rapid，and could simplify the determination of the single components in multi-component system.