Preparation and in vitro evaluation of vincristine-loaded superparamagnetic iron oxide nanoparticles
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Abstract
Vincristine loaded superparamagnetic iron oxide (VCR-SPIO) nanoparticles were prepared by self-assembly process.Phenanthroline spectrophotometry and HPLC were used respectively to determine the concentration of iron and vincristine of the pharmaceutical preparation.Encapsulation efficiency was calculated.The morphology and size of the preparation were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS).Vibrating sample magnetometer (VSM) was used to detect saturation magnetization,and magnetization curve was drawn.Human leukemia K562 cells were cultured in the presence of vincristine sulfate and VCR-SPIO nanoparticles.Viability of cells was determined by CCK-8.It was found that encapsulation efficiency was (81.2±1.5) %.The concentration of iron was (104±1.4)μg/mL.The average particle diameter of the preparation was (83±1.2) nm.VCR-SPIO nanoparticles had superparamagnetic effect with the saturation magnetization of 56.3 A·m2/kg.VCR-SPIO nanoparticles showed higher cytotoxicity on K562 cells.It was shown in this study that the novel multifunctional superparamagnetic iron oxide nanoparticles as a novel drug delivery system had the potential for cancer therapy.
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