硫酸呈色－分光光度法测定红霉素片的溶出度

Determination of Erythromycin Content in Intestinal Juice Using Color Development Reaction-Spectrophotometry

摘要: 对硫酸呈色－分光光度法测定红霉素片溶出度含量进行了研究。结果表明，呈色液色泽的深浅，可从分光光度计４９０ｎｍ波长处测定的吸收度，反映出溶出药含量之变化，符合Ｂｅｅｒ定律。线性范围为３０～１２４ｕ／ｍｌ，ｙ＝０．３８２９＋０．０３２９．ｒ＝０．９９９９，Ｐ＜０．０１，回收率ＲＳＤ为９９．４％±０．０４４％。与已报道的其它方法相比，本法具有无需萃取、不受辅料干扰、操作简便以及重现性好等优点。

Abstract: A method for quantitative analysis of erythromycin tablet dissolved in simulated intestinal juice was described. In the experiment,the sample extraction could be omitted,so the filtered portion of the solution under test could be treated directly with con