药用乳糖酶酵母菌发酵条件研究

Fermentation Studies on Yeast Producing High Level of Lactase

摘要: 依据微生物中普遍存在的葡萄糖效应原理,结合正交试验法优化设计确定了乳糖酶高产酵母菌Y34440的发酵培养基组分为乳糖（2％）,葡萄糖（2％）,玉米浆（2％）,酵母汁（0.5％）,尿素（1.5％）,并优化了其发酵工艺。该培养基价格低廉,产酶活高,适宜作为工业发酵用培养基。20L发酵罐（装液量16L）发酵试验结果表明,发酵液最高产酶量达9.18ONPG单位／ml。

Abstract: A low-priced medium for producing lactase was designed by microbiological principle of glucose effect and orthogonal design method. Some fermentation conditions were optAnized and the fermentation in 20 liter of fermentation showed that the yeast strain K