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HPLC法同时测定芦根中对香豆酸和阿魏酸含量

潘春燕, 陈静, 杭太俊, 宋敏

潘春燕, 陈静, 杭太俊, 宋敏. HPLC法同时测定芦根中对香豆酸和阿魏酸含量[J]. 中国药科大学学报, 2015, 46(2): 219-223. DOI: 10.11665/j.issn.1000-5048.20150214
引用本文: 潘春燕, 陈静, 杭太俊, 宋敏. HPLC法同时测定芦根中对香豆酸和阿魏酸含量[J]. 中国药科大学学报, 2015, 46(2): 219-223. DOI: 10.11665/j.issn.1000-5048.20150214
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PAN Chunyan, CHEN Jing, HANG Taijun, SONG Min. Simultaneous determination of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC[J]. Journal of China Pharmaceutical University, 2015, 46(2): 219-223. DOI: 10.11665/j.issn.1000-5048.20150214
Citation: PAN Chunyan, CHEN Jing, HANG Taijun, SONG Min. Simultaneous determination of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC[J]. Journal of China Pharmaceutical University, 2015, 46(2): 219-223. DOI: 10.11665/j.issn.1000-5048.20150214
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HPLC法同时测定芦根中对香豆酸和阿魏酸含量

  • 中国药科大学药物分析学教研室

Simultaneous determination of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC

摘要

    摘要
  • 摘要: 采用HPLC法同时测定对香豆酸和阿魏酸的含量以评价芦根质量。芦根经碱性乙醇水溶液回流提取对香豆酸和阿魏酸，Diamonsil C18柱(4.6 mm×250 mm，5 μm)，乙腈-0.3%醋酸水溶液流动相梯度洗脱分离，紫外310 nm波长检测。对香豆酸和阿魏酸分别在0.2~200 μg/mL和0.1~120 μg/mL范围有良好线性，平均回收率分别为(96.9±2.91)%和(98.7±1.78)%。测得15批市售芦根饮片中对香豆酸和阿魏酸的含量(±s，max~min)分别为(0.88±0.16，1.21~0.70)%和(0.41±0.035，0.49~0.36)%。所建立的对香豆酸和阿魏酸含量的同时测定方法可用于芦根质量的评价。
    Abstract: To determine the contents of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC for quality evaluation. Rhizama Phragmitis alkaline alcoholic solution exact of p-coumaric acid and ferulic acid was refluxed. An HPLC method was developed by using a Diamonsil C18(4. 6 mm×250 mm, 5 μm)column, and the mobile phase of acetonitrile and 0. 3% acetic acid with linear gradient elution at a flow of 1. 0 mL/min and UV detection at 310 nm. Calibration curvers of p-coumaric acid and ferulic acid were linear over the range of 0. 2-200 μg/mL and 0. 1-120 μg/mL respectively. . Average recoveries were(96. 9±2. 91)% and(98. 7±1. 78)%, respectively. The average contents of p-coumaric acid and ferulic acid expressed as mean±sd(max-min)were(0. 88±0. 16)%(1. 21%-0. 70%)and(0. 41±0. 035)%(0. 49%-0. 36%)in 15 batches of Rhizama Phragmitis. The established method will be beneficial to the quality evaluation of Rhizama Phragmitis.
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  • 刊出日期:  2015-04-24

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