FGF21对鱼藤酮导致神经细胞损伤的作用及其机制

陈映洁; 高向东; 陈松

doi:10.11665/j.issn.1000-5048.20200611

FGF21对鱼藤酮导致神经细胞损伤的作用及其机制

中国药科大学生命科学与技术学院江苏省生物药物成药性研究重点实验室，南京 211198

基金项目: 国家自然科学基金资助项目(No.81673435，No.81872850)；“双一流”大学资助项目(No.CPU2018GF08)

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出版历程
- 收稿日期: 2020-05-12
- 修回日期: 2020-09-23
- 刊出日期: 2020-12-24

Effects and mechanisms of FGF21 on neuronal damage induced by rotenone

Funds: This study was supported by the National Natural Science Foundation of China (No. 81673435,No.81872850) and the

摘要

摘要: 采用鱼藤酮诱导SH-SY5Y细胞损伤建立帕金森病样病变细胞模型，探讨成纤维细胞生长因子21（fibroblast growth factor 21，FGF21）对其作用及机制。以不同浓度的FGF21对鱼藤酮诱导的神经细胞损伤模型进行干预，MTT法检测神经细胞活性；采用Annexin V-FITC/PI双染法分析神经细胞凋亡情况；Western blot检测FGF21及鱼藤酮对神经细胞酪氨酸羟化酶（tyrosine hydroxylase，TH）和α-突触核蛋白（α-synuclein，α-syn）表达的影响；采用DCFH-DA荧光探针检测FGF21及鱼藤酮对神经细胞内活性氧（reactive oxygen species，ROS）水平的作用。结果显示：FGF21能够减少鱼藤酮致神经细胞的损伤，抑制神经细胞凋亡，缓解鱼藤酮引起的神经细胞TH和α-syn水平的异常，同时降低神经细胞内异常ROS水平，提示FGF21可能通过调控氧化应激进而缓解鱼藤酮导致的神经细胞损伤。
- 成纤维细胞生长因子21 /
- 帕金森病 /
- 鱼藤酮 /
- 细胞凋亡 /
- 活性氧
Abstract: In this study,the SH-SY5Y cell model with Parkinson"s disease-like lesions was established by using rotenone,and the effect and mechanism of fibroblast growth factor 21 (FGF21) on the cell model were explored. Different concentrations of FGF21 were used to treat neuronal injury model induced by rotenone,and cell viabilities were detected by MTT assay. Effects of FGF21 and rotenone on the apoptotic levels of SH-SY5Y cells were analyzed by using Annexin V-FITC detection kit. Western blot was used to assess the effects of FGF21 and rotenone on the protein levels of tyrosine hydroxylase (TH) and α-synuclein (α-syn) in SH-SY5Y cells. Effects of FGF21 and rotenone on reactive oxygen species (ROS) levels in SH-SY5Y cells were tested using DCFH-DA fluorescent probe. The results showed that FGF21 could reduce the damage in SH-SY5Y cells induced by rotenone,inhibit cell apoptosis,alleviate the abnormalities of TH and α-syn in SH-SY5Y cells induced by rotenone and down-regulate the abnormal ROS levels in SH-SY5Y cells. The results suggested that FGF21 may attenuate rotenone-induced neuronal damage through regulation of oxidative stress.
- fibroblast growth factor 21 /
- Parkinson"s disease /
- rotenone /
- apoptosis /
- reactive oxygen species

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