金刚纂中木脂素对食管鳞癌细胞的抑制作用
Suppressive effect of lignans from Euphorbia neriifolia L.on esophageal squamous cancer cell
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摘要: 金刚纂（Euphorbia neriifolia L.）是大戟科植物，采用硅胶柱色谱以及制备HPLC等多种色谱技术，从金刚纂地上部分中分离出5个已知的木脂素成分，并对这5种木脂素的潜在抗肿瘤活性进行体外研究，其中化合物2对食管鳞癌细胞，特别是KYSE-410和KYSE-450细胞，表现出增殖抑制和细胞毒性。机制研究进一步证明化合物2可通过激活caspase-3/9及下调Bcl-2/Bax蛋白表达比例，诱导癌细胞发生显著凋亡。实验结果显示，化合物2在体外对食管鳞癌细胞，尤其是KYSE-410细胞具有显著抑制作用，表明化合物2具有良好的抗肿瘤活性，应对其进行深入研究。Abstract: Eubhorbia neriifolia L. is a plant of Euphorbia family.Five known lignans were isolated from the aerial parts of E. neriifolia L. by silica gel for column chromatography and preparative high-performance liquid chromatography (HPLC).Their potential antitumor activities were evaluated in vitro.Compound 2 exhibited proliferation inhibition and cytotoxicity against esophageal squamous cancer cells, especially KYSE-410 and KYSE-450 cells.Further analyses showed that compound 2 could significantly induce apoptosis through the activation of caspase 3/9 and down-regulation of the Bcl-2/Bax protein ratio.These results suggested that compound 2 had a significant inhibitory effect on the esophageal squamous cancer cells, especially KYSE-410, which deserves further research as a potential antitumor agent.
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Keywords:
- Euphorbia neriifolia L. /
- lignans /
- antitumor activity /
- cell apoptosis
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