• 中国中文核心期刊
  • 中国科学引文数据库核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
高级检索

乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制

杨甲丽, 贺升升, 柯慧, 杨珊, 王喆堃, 张兴江, 王攀, 宗凯乐, 黄庆, 吴建新

杨甲丽, 贺升升, 柯慧, 杨珊, 王喆堃, 张兴江, 王攀, 宗凯乐, 黄庆, 吴建新. 乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制[J]. 中国药科大学学报, 2023, 54(2): 188-197. DOI: 10.11665/j.issn.1000-5048.2023030302
引用本文: 杨甲丽, 贺升升, 柯慧, 杨珊, 王喆堃, 张兴江, 王攀, 宗凯乐, 黄庆, 吴建新. 乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制[J]. 中国药科大学学报, 2023, 54(2): 188-197. DOI: 10.11665/j.issn.1000-5048.2023030302
shu
YANG Jiali, HE Shengsheng, KE Hui, YANG Shan, WANG Zhekun, ZHANG Xingjiang, WANG Pan, ZONG Kaile, HUANG Qing, WU Jianxin. Anti-senescence effect of acetyl dipeptide-1 cetyl ester on skin and its mechanism[J]. Journal of China Pharmaceutical University, 2023, 54(2): 188-197. DOI: 10.11665/j.issn.1000-5048.2023030302
Citation: YANG Jiali, HE Shengsheng, KE Hui, YANG Shan, WANG Zhekun, ZHANG Xingjiang, WANG Pan, ZONG Kaile, HUANG Qing, WU Jianxin. Anti-senescence effect of acetyl dipeptide-1 cetyl ester on skin and its mechanism[J]. Journal of China Pharmaceutical University, 2023, 54(2): 188-197. DOI: 10.11665/j.issn.1000-5048.2023030302
shu

乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制

  • 中国药科大学中药学院，南京 211198

基金项目: 中国药科大学高层次人才引进科研启动经费项目资助（No.3150020057）

Anti-senescence effect of acetyl dipeptide-1 cetyl ester on skin and its mechanism

  • School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China

Funds: This research was supported by the Start-up Fund of China Pharmaceutical University (No. 3150020057)

摘要

    摘要
  • 摘要: 乙酰基二肽-1鲸蜡酯（acetyl dipeptide-1 cetyl ester，AD-1）是乙酸和十六烷基醇与精氨酸和酪氨酸组成的人工合成肽，具有一定的抗炎和提高皮肤屏障功能的作用，已被用于敏感肌化妆品中。该成分虽然也被用于抗衰老化妆品中，但缺乏公开的系统科学研究。本研究通过评价AD-1的体外抗氧化和抗糖基化作用、建立UVA反复照射真皮原代成纤维细胞的光老化模型，探讨其在光老化细胞中的抗衰老活性。采用MTT法测定AD-1对细胞活力的影响；RT-qPCR法检测AD-1和UVA对衰老相关p21、p53、MMPs、IL6、Col1、Col3以及自噬相关p62、ATG5、ATG7 mRNA表达的影响；Western blot法检测AD-1和UVA对衰老及自噬相关p16、p21、p53、Col1、LC3B、p62蛋白水平的影响；β-半乳糖苷酶染色和MDC染色评价细胞衰老损伤，并通过荧光探针DCFH-DA检测细胞内活性氧水平。实验结果显示，AD-1干预可以减少UVA诱导的细胞损伤和mRNA表达异常，改善p16、p21、p53、Col1、LC3B、p62蛋白水平异常。结果表明，AD-1具有一定的体外抗氧化、抗糖基化能力，同时可能通过激活自噬保护UVA诱导的光老化细胞，具有较好的抗衰老活性。
    Abstract: Acetyl dipeptide-1 cetyl ester (AD-1) is a synthetic peptide composed of acetic acid and cetyl alcohol with arginine and tyrosine, which has certain anti-inflammatory and skin barrier enhancement effects, has been used in cosmetics for sensitive skin.Meanwhile, the ingredient has also been used in anti-aging cosmetics, but there is a lack of published scientific evidence on anti- senescence aspect.In this study, we investigated the related effects of AD-1 by evaluating its in vitro antioxidant and antiglycation efficacies.Furthermore, we established a photoaging model on primary rat dermal fibroblasts by repeated exposures to UVA irradiation.MTT assay was used to detect the effects of AD-1 on the cell viability.RT-qPCR was used to determine the effects of AD-1 on the mRNA levels of senescence-related p21, p53, MMPs, IL6, Col1, Col3 and autophagy-related p62, ATG5, ATG7.Western blot was used to detect the effects of AD-1 on the protein levels of p16, p21, p53, Col1, LC3B and p62.SA-β-gal was performed to indicate senescence level of the cell.MDC was performed to indicate autophagy level.Intracellular reactive oxygen species were monitored by fluorescent probes DCFH-DA.The results showed that AD-1 could reduce UVA-induced the cell damage and regulate the abnormal expression of mRNA levels. It alleviated the abnormal protein levels of p16, p21, p53, Col1, LC3B and p62 induced by UVA. These results suggested that AD-1 has not only antioxidant and antiglycation effects but also can activate autophagy to achieve anti-senescence effect.
    • HTML全文
    • 参考文献(37)
  • [1] Leidal AM, Levine B, Debnath J. Autophagy and the cell biology of age-related disease[J]. Nat Cell Biol, 2018, 20(12): 1338-1348.
    [2] López-Otín C, Blasco MA, Partridge L, et al. The hallmarks of aging[J]. Cell, 2013, 153(6): 1194-1217.
    [3] Zhang Y, Liu XY, Wang JP, et al. Analysis of multi-part phenotypic changes in skin to characterize the trajectory of skin aging in Chinese women[J]. Clin Cosmet Investig Dermatol, 2022, 15: 631-642.
    [4] Lee H, Hong YJ, Kim M. Structural and functional changes and possible molecular mechanisms in aged skin[J]. Int J Mol Sci, 2021, 22(22): 12489.
    [5] Rittié L, Fisher GJ. Natural and sun-induced aging of human skin[J]. Cold Spring Harb Perspect Med, 2015, 5(1): a015370.
    [6] Tobin DJ. Introduction to skin aging[J]. J Tissue Viability, 2017, 26(1): 37-46.
    [7] Yang XX, Zhao MM, He YF, et al. Facial skin aging stages in Chinese females[J]. Front Med (Lausanne), 2022, 9: 870926.
    [8] Kovács M, Podda M. Hautalterung und dermatologische pathologien[J]. J Für ?sthetische Chir, 2021, 14(2): 68-73.
    [9] Pedi? L, Pondeljak N, ?itum M. Recent information on photoaging mechanisms and the preventive role of topical sunscreen products[J]. Acta Dermatovenerol Alp Pannonica Adriat, 2020, 29(4): 201-207.
    [10] Wang YJ, Wang L, Wen X, et al.NF-κB signaling in skin aging[J]. Mech Ageing Dev, 2019, 184: 111160.
    [11] Kohl E, Steinbauer J, Landthaler M, et al.Skin ageing[J]. J Eur Acad Dermatol Venereol, 2011, 25(8): 873-884.
    [12] Rubinsztein D, Mari?o G, Kroemer G. Autophagy and aging[J]. Cell, 2011, 146(5): 682-695.
    [13] Martins de Andrade V, Bardají E, Heras M, et al. Antifungal and anti-biofilm activity of designed derivatives from kyotorphin[J]. Fungal Biol, 2020, 124(5): 316-326.
    [14] Sulzberger M, Worthmann AC, Holtzmann U, et al. Effective treatment for sensitive skin: 4-t-butylcyclohexanol and licochalcone A[J]. J Eur Acad Dermatol Venereol, 2016, 30: 9-17.
    [15] Resende DISP, Ferreira MS, Sousa-Lobo JM, et al. Usage of synthetic peptides in cosmetics for sensitive skin[J]. Pharmaceuticals (Basel), 2021, 14(8): 702.
    [16] Ferreira MS, Sousa Lobo JM, Almeida IF. Sensitive skin: active ingredients on the spotlight[J]. Int J Cosmetic Sci, 2022, 44(1): 56-73.
    [17] Nakyai W, Saraphanchotiwitthaya A, Viennet C, et al. An in vitro model for fibroblast photoaging comparing single and repeated UVA irradiations[J]. Photochem Photobiol, 2017, 93(6): 1462-1471.
    [18] Endo K, Katsuyama Y, Taira N, et al. Impairment of the autophagy system in repetitively UVA-irradiated fibroblasts[J]. Photodermatol Photoimmunol Photomed, 2020, 36(2): 111-117.
    [19] Wen WJ, Chen JW, Ding LG, et al. Astragaloside exerts anti-photoaging effects in UVB-induced premature senescence of rat dermal fibroblasts through enhanced autophagy[J]. Arch Biochem Biophys, 2018, 657: 31-40.
    [20] Zhang M, Zhang T, Tang YN, et al. Concentrated growth factor inhibits UVA-induced photoaging in human dermal fibroblasts via the MAPK/AP-1 pathway[J]. Biosci Rep, 2020, 40(7): BSR20193566.
    [21] Harman D. Aging: a theory based on free radical and radiation chemistry[J]. J Gerontol, 1956, 11(3): 298-300.
    [22] Zheng WG, Li HJ, Go Y, et al. Research advances on the damage mechanism of skin glycation and related inhibitors[J]. Nutrients, 2022, 14(21): 4588.
    [23] Kaeberlein M, Tyler JK. A new era for research into aging[J]. eLife, 2021, 10: e65286.
    [24] Szychowski KA, Skóra B. Review of the relationship between reactive oxygen species (ROS) and elastin-derived peptides (EDPs)[J]. Appl Sci (Basel), 2021, 11(18):8732.
    [25] Fitsiou E, Pulido T, Campisi J, et al. Cellular senescence and the senescence associated secretory phenotype as drivers of skin photoaging[J]. J Investig Dermatol, 2021, 141(4): 1119-1126.
    [26] Bernerd F, Passeron T, Castiel I, et al. The damaging effects of long UVA (UVA1) rays: a major challenge to preserve skin health and integrity[J]. Int J Mol Sci, 2022, 23(15): 8243.
    [27] Qin DK, Ren RJ, Jia CL, et al. Rapamycin protects skin fibroblasts from ultraviolet B-induced photoaging by suppressing the production of reactive oxygen species[J]. Cell Physiol Biochem, 2018, 46(5): 1849-1860.
    [28] Salminen A, Kaarniranta K, Kauppinen A. Photoaging: UV radiation-induced inflammation and immunosuppression accelerate the aging process in the skin[J]. Inflamm Res, 2022, 71(7): 817-831.
    [29] Cole MA, Quan TH, Voorhees JJ, et al.Extracellular matrix regulation of fibroblast function: redefining our perspective on skin aging[J]. J Cell Commun Signal, 2018, 12(1): 35-43.
    [30] Gu YP, Han JX, Jiang CP, et al. Biomarkers, oxidative stress and autophagy in skin aging[J]. Ageing Res Rev, 2020, 59: 101036.
    [31] Kaushik S, Tasset I, Arias E, et al. Autophagy and the hallmarks of aging[J]. Ageing Res Rev, 2021, 72: 101468.
    [32] Li TZ, Zhang HL, Wang ZC, et al. The regulation of autophagy by the miR-199a-5p/p62 axis was a potential mechanism of small cell lung cancer cisplatin resistance[J]. Cancer Cell Int, 2022, 22(1): 120.
    [33] Jeong D, Qomaladewi NP, Lee J, et al. The role of autophagy in skin fibroblasts, keratinocytes, melanocytes, and epidermal stem cells[J]. J Investig Dermatol, 2020, 140(9): 1691-1697.
    [34] Ma JW, Teng Y, Huang YM, et al. Autophagy plays an essential role in ultraviolet radiation-driven skin photoaging[J]. Front Pharmacol, 2022, 13: 864331.
    [35] Xie H, Zhou L, Liu F, et al. Autophagy induction regulates aquaporin 3-mediated skin fibroblast ageing[J]. Br J Dermatol, 2022, 186(2): 318-333.
    [36] Lim GE, Park JE, Cho YH, et al. Alpha-neoendorphin can reduce UVB-induced skin photoaging by activating cellular autophagy[J]. Arch Biochem Biophys, 2020, 689: 108437.
    [37] Khmaladze I, ?sterlund C, Smiljanic S, et al. A novel multifunctional skin care formulation with a unique blend of antipollution, brightening and antiaging active complexes[J]. J Cosmet Dermatol, 2020, 19(6): 1415-1425.
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  556
  • HTML全文浏览量:  58
  • PDF下载量:  480
  • 被引次数: 0
出版历程
  • 收稿日期:  2023-03-02
  • 修回日期:  2023-04-02
  • 刊出日期:  2023-04-24

目录

摘要
HTML全文
    参考文献(37)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    版权所有：《中国药科大学学报》编辑部苏ICP备05007142号

    地址：江苏省南京市鼓楼区童家巷24号（210009）电话: 025-83271566E-mail: xuebao@cpu.edu.cn

    本系统由 北京仁和汇智信息技术有限公司 开发  百度统计

    访问量：365535

    x 关闭 永久关闭