乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制

杨甲丽; 贺升升; 柯慧; 杨珊; 王喆堃; 张兴江; 王攀; 宗凯乐; 黄庆; 吴建新

doi:10.11665/j.issn.1000-5048.2023030302

乙酰基二肽-1鲸蜡酯的皮肤抗衰老作用及其机制

中国药科大学中药学院，南京 211198

基金项目: 中国药科大学高层次人才引进科研启动经费项目资助（No.3150020057）

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出版历程
- 收稿日期: 2023-03-02
- 修回日期: 2023-04-02
- 刊出日期: 2023-04-24

Anti-senescence effect of acetyl dipeptide-1 cetyl ester on skin and its mechanism

School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China

Funds: This research was supported by the Start-up Fund of China Pharmaceutical University (No. 3150020057)

摘要

摘要: 乙酰基二肽-1鲸蜡酯（acetyl dipeptide-1 cetyl ester，AD-1）是乙酸和十六烷基醇与精氨酸和酪氨酸组成的人工合成肽，具有一定的抗炎和提高皮肤屏障功能的作用，已被用于敏感肌化妆品中。该成分虽然也被用于抗衰老化妆品中，但缺乏公开的系统科学研究。本研究通过评价AD-1的体外抗氧化和抗糖基化作用、建立UVA反复照射真皮原代成纤维细胞的光老化模型，探讨其在光老化细胞中的抗衰老活性。采用MTT法测定AD-1对细胞活力的影响；RT-qPCR法检测AD-1和UVA对衰老相关p21、p53、MMPs、IL6、Col1、Col3以及自噬相关p62、ATG5、ATG7 mRNA表达的影响；Western blot法检测AD-1和UVA对衰老及自噬相关p16、p21、p53、Col1、LC3B、p62蛋白水平的影响；β-半乳糖苷酶染色和MDC染色评价细胞衰老损伤，并通过荧光探针DCFH-DA检测细胞内活性氧水平。实验结果显示，AD-1干预可以减少UVA诱导的细胞损伤和mRNA表达异常，改善p16、p21、p53、Col1、LC3B、p62蛋白水平异常。结果表明，AD-1具有一定的体外抗氧化、抗糖基化能力，同时可能通过激活自噬保护UVA诱导的光老化细胞，具有较好的抗衰老活性。
- 抗衰老 /
- 皮肤 /
- 乙酰基二肽-1鲸蜡酯 /
- 自噬
Abstract: Acetyl dipeptide-1 cetyl ester (AD-1) is a synthetic peptide composed of acetic acid and cetyl alcohol with arginine and tyrosine, which has certain anti-inflammatory and skin barrier enhancement effects, has been used in cosmetics for sensitive skin.Meanwhile, the ingredient has also been used in anti-aging cosmetics, but there is a lack of published scientific evidence on anti- senescence aspect.In this study, we investigated the related effects of AD-1 by evaluating its in vitro antioxidant and antiglycation efficacies.Furthermore, we established a photoaging model on primary rat dermal fibroblasts by repeated exposures to UVA irradiation.MTT assay was used to detect the effects of AD-1 on the cell viability.RT-qPCR was used to determine the effects of AD-1 on the mRNA levels of senescence-related p21, p53, MMPs, IL6, Col1, Col3 and autophagy-related p62, ATG5, ATG7.Western blot was used to detect the effects of AD-1 on the protein levels of p16, p21, p53, Col1, LC3B and p62.SA-β-gal was performed to indicate senescence level of the cell.MDC was performed to indicate autophagy level.Intracellular reactive oxygen species were monitored by fluorescent probes DCFH-DA.The results showed that AD-1 could reduce UVA-induced the cell damage and regulate the abnormal expression of mRNA levels. It alleviated the abnormal protein levels of p16, p21, p53, Col1, LC3B and p62 induced by UVA. These results suggested that AD-1 has not only antioxidant and antiglycation effects but also can activate autophagy to achieve anti-senescence effect.
- anti-senescence /
- skin /
- acetyl dipeptide-1 cetyl ester /
- autophagy

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