Advanced Search
LOU Bin, WANG Yunshan, GU Minglin, WANG Dawei. Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pathway[J]. Journal of China Pharmaceutical University, 2019, 50(6): 728-733. DOI: 10.11665/j.issn.1000-5048.20190614
Citation: LOU Bin, WANG Yunshan, GU Minglin, WANG Dawei. Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pathway[J]. Journal of China Pharmaceutical University, 2019, 50(6): 728-733. DOI: 10.11665/j.issn.1000-5048.20190614

Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pathway

More Information
  • To investigate the mechanism of Shouwu Jiangzhi decoction in treatment of hyperlipidemia by suppress apoB-48 in small intestines, Golden Syrian hamsters were randomly devided into blank group, model group, fenobrate treatment group and Shouwu Jiangzhi decoction treatment group based on weight. The hyperlipidemia models of golden Syrian hamsters were induced by high fat diet(HFD)treatment for 4 weeks, then administered orally with drugs for 4 weeks. The serum indexes of HDL-C, LDL-C, TG and TC were determined by microplate methods, ELISA kits were used to evaluate the contents of serum TNF-α, apoB-48 and FFA. The protein expression levels of p38, ERK, JNK, SREBP, TNF-α and apoB-48 in small intestines were determined by Western blots. The results showed that Shouwu Jiangzhi decoction can effectively increase the serum HDL-C level and reduce the serum level of TG, LDL-C, TNF-α and apoB-48 in HFD-induced hamsters. Furthermore, Shouwu Jiangzhi decoction can significantly downregulate the protein expressions of p38, JNK, ERK, SREBP, TNF-α and apoB-48 in small intestines. Results above indicate that Shouwu Jiangzhi decoction may downregulate the protein expression of apoB-48 to treat hyperlipidemia via partially downregulating TNF-α/MAPK signal pathway.
  • [1]
    Heine RJ.The role of postprandial hyperglycemia and hyperlipidemia in CVD risk[J].Curr Diab Rep,2006,6(1):1-2.
    [2]
    Hu DY.New guidelines and evidence for prevention and treatment of dyslipidemia and atherosclerotic cardiovascular disease in China[J].Chronic Dis Transl Med,2017,3(2):73-74.
    [3]
    Huang YX,Gao L,Xie XP,et al.Epidemiology of dyslipidemia in Chinese adults:meta-analysis of prevalence,awareness,treatment,and control[J].Popul Health Metr,2014,12(1):28.
    [4]
    Lindman AS,Veierød MB,Tverdal A,et al.Nonfasting triglycerides and risk of cardiovascular death in men and women from the norwegian counties study[J].Eur J Epidemiol,2010,25(11):789-798.
    [5]
    Nordestgaard BG,Benn M,Schnohr P,et al.Nonfasting triglycerides and risk of myocardial infarction,ischemic heart disease,and death in men and women[J].JAMA,2007,298(3):299.
    [6]
    Nakajima K,Nagamine T,Fujita MQ,et al.Apolipoprotein B-48:a unique marker of chylomicron metabolism[J].Adv Clin Chem,2014,64:117-177.
    [7]
    Qin BL,Qiu W,Avramoglu RK,et al.Tumor necrosis factor-alpha induces intestinal insulin resistance and stimulates the overproduction of intestinal apolipoprotein B48-containing lipoproteins[J].Diabetes,2007,56(2):450-461.
    [8]
    van Breukelen-van der Stoep D,van Zeben D,de Vries M,et al.Plasma apolipoprotein B48 levels in patients with rheumatoid arthritis:evaluating novel cardiovascular risk factors[J].Arthritis Rheumatol,2015,67:2.
    [9]
    Federico LM,Naples M,Taylor D,et al.Intestinal insulin resistance and aberrant production of apolipoprotein B48 lipoproteins in an animal model of insulin resistance and metabolic dyslipidemia:evidence for activation of protein tyrosine phosphatase-1B,extracellular signal-related kinase,and sterol regulatory element-binding protein-1c in the fructose-fed hamster intestine[J].Diabetes,2006,55(5):1316-1326.
    [10]
    Lou B,Wang DC,Xu CB,et al.Clinical efficacy of Shouwu Jiangzhi tablets in treating hyperlipemia[J].Chin J Tradit Chin Med Pharm(中华中医药学刊),2007,25(2):315-316.
    [11]
    Lou DX,Yan D,Guo M,et al.Antipyretic effect of isodon eriocalyx extracts on yeast-induced fever in rats [J].J China Pharm Univ(中国药科大学学报),2019,50(1):87-92.
    [12]
    Eberlé D,Hegarty B,Bossard P,et al.SREBP transcription factors:master regulators of lipid homeostasis[J].Biochimie,2004,86(11):839-848.
    [13]
    Yoshimura N,Kinoshita M,Teramoto T.Isolation and characterization of apolipoprotein B48-containing lipoproteins with a monoclonal antibody against apolipoprotein B48[J].J Atheroscler Thromb,2009,16(6):740-747.
    [14]
    Choi RY,Lee HI,Ham JR,et al.Heshouwu(Polygonum multiflorum Thunb.)ethanol extract suppresses pre-adipocytes differentiation in 3T3-L1 cells and adiposity in obese mice[J].Biomedecine Pharmacother,2018,106:355-362.
    [15]
    Zhu HF,Wang Y,Liu ZQ,et al.Antidiabetic and antioxidant effects of catalpol extracted from Rehmannia glutinosa(Di Huang)on rat diabetes induced by streptozotocin and high-fat,high-sugar feed[J].Chin Med,2016,11:25.
    [16]
    Kwok CY,Wong CNY,Yau MYC,et al.Consumption of dried fruit of Crataegus pinnatifida(hawthorn)suppresses high-cholesterol diet-induced hypercholesterolemia in rats[J].J Funct Foods,2010,2(3):179-186.
    [17]
    Wang T,An YT,Zhao CF,et al.Regulation effects of Crataegus pinnatifida leaf on glucose and lipids metabolism[J].J Agric Food Chem,2011,59(9):4987-4994.
    [18]
    Shui YM,Lv GY,Shan LT,et al.Epimedin C promotes vascularization during BMP2-induced osteogenesis and tumor-associated angiogenesis[J].Am J Chin Med,2017,45(5):1093-1111.
    [19]
    Li XY,Peng BJ,Pan YB,et al.Icariin stimulates osteogenic differentiation and suppresses adipogenic differentiation of rBMSCs via estrogen receptor signaling[J].Mol Med Rep,2018,18(3):3483-3489.
  • Cited by

    Periodical cited type(2)

    1. 杨坤,张海珠,张月辉,范科琴,宋方宇,马丙锁,张良明,晏子俊. 叶酸修饰的pH值响应型去甲斑蝥素脂质体的制备及促HepG2细胞凋亡研究. 药物评价研究. 2025(06): 1529-1545 .
    2. 张文典,崔杰,夏一帆,段少峰. 叶酸靶向anti-miR-221阴离子脂质体的制备及体外抗肿瘤作用. 中国药房. 2022(07): 813-817 .

    Other cited types(0)

Catalog

    Article views (369) PDF downloads (636) Cited by(2)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return