Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography

An ultra-performance liquid chromatographic method was established for the simultaneous determination of seven active compounds in Huoxiang Zhengqi liquid. Seven compounds were analyzed simultaneously with an Acquity BEH C₁₈ column by gradient elution using acetonitrile-0. 1% phosphoric acid as the mobile phase. The column was maintained at 35 °C; the flow rate was 0. 4 mL/min; the detection wavelengths were set at 249, 284 and 336 nm, respectively. Good linearity(r> 0. 999 9)in the range of the test concentration was observed between concentration and chromatographic response of each compound, and the average recoveries of the method were between 96. 16% and 103. 4%.