• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
LI Hui, WEN Shuping, HONG Wenrong. Construction of carbamoyltobramcin-producing engineering S.tenebrarius by disruption of aprK gene[J]. Journal of China Pharmaceutical University, 2013, 44(4): 368-373. DOI: 10.11665/j.issn.1000-5048.20130416
Citation: LI Hui, WEN Shuping, HONG Wenrong. Construction of carbamoyltobramcin-producing engineering S.tenebrarius by disruption of aprK gene[J]. Journal of China Pharmaceutical University, 2013, 44(4): 368-373. DOI: 10.11665/j.issn.1000-5048.20130416

Construction of carbamoyltobramcin-producing engineering S.tenebrarius by disruption of aprK gene

More Information
  • To construct a genetic engineering S. tenebrarius for production of carbamoyltobramycin. Recombinant plasmid pBK5 derivated from pKC1139 was constructed for disrupting gene aprK. pBK5 was introduced into S. tenebrariusTt-49 by conjugation. The single crossover mutant ST315 was obtained and cultivated for several rounds of sporulation in the absence of erythromycin. A desired double crossover mutant ST316 was achieved by PCR. TLC analysis indicated that ST316 produced high-yield carbamoyltobramycin, in which apramycin biosynthesis was blocked and the yield of carbamoyltobramycin was about 1 500 ug/mL.
  • Related Articles

    [1]WANG Xuefang, ZHAO Yang, LIU Zhuqing, GUO Le, ZHONG Feiliang, LUO Xuegang. Genetic engineering and molecular modification of recombinant fully humanized single-domain antibody against Helicobacter pylori UreB[J]. Journal of China Pharmaceutical University, 2024, 55(5): 666-672. DOI: 10.11665/j.issn.1000-5048.2023122903
    [2]LIN Yu-shuang, HONG Wen-rong. Construction of Streptomyces tenebrarius mutant with knockout of tobS1-C genes[J]. Journal of China Pharmaceutical University, 2012, 43(1): 92-96.
    [4]Fermentation Conditions for High Level Expression of Novel Human Calcitonin Analogue Gene in Engineering Strain[J]. Journal of China Pharmaceutical University, 2000, (2): 74-77.
    [9]Primary Studies on the Immobilization of an Engineering E. coli Strain with High Activity of Aromatic Amino Acid Transaminase[J]. Journal of China Pharmaceutical University, 1994, (5): 316-318.
    [10]The Construction of an E.coli Strain with Expression of Fumarate Hydratase[J]. Journal of China Pharmaceutical University, 1994, (3): 159-162.

Catalog

    Article views (1587) PDF downloads (2360) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return