Effect of combretastatin A-4 derivative CPU-XT-006 on proliferation, apoptosis and expression of VEGF and bFGF of HUVEC in vitro

Compound CPU-XT-006 is a derivative of combretastatin A-4(CA-4), one kind of microtubule protein polymerization inhibitors and vascular blocking agents. MTT assay was used to detect the effect of CPU-XT-006 on the multiplication of HUVEC induced by newborn calf serum, vascular endothelial gronth factor(VEGF)or basic fibroblast growth tactor(bFGF)respectively. Flow cytometry was used to detect the HUVEC apoptosis caused by CPU-XT-006. The expression of VEGF and bFGF protein was detected by Western blot. The results showed that the compound CPU-XT-006 could inhibit HUVEC proliferation, induce HUVEC apoptosis and reduce expression of VEGF and bFGF protein.