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WANG Jing, HUO Meirong, ZHOU Jianping, ZHANG Yong, ZHANG Xuyuan, WANG Lei, YIN Tingjie. Drug-loaded micelles based on hyaluronic acid-paclitaxel prodrug: preparation and pharmacokinetic study in rats[J]. Journal of China Pharmaceutical University, 2013, 44(6): 520-525. DOI: 10.11665/j.issn.1000-5048.20130606
Citation: WANG Jing, HUO Meirong, ZHOU Jianping, ZHANG Yong, ZHANG Xuyuan, WANG Lei, YIN Tingjie. Drug-loaded micelles based on hyaluronic acid-paclitaxel prodrug: preparation and pharmacokinetic study in rats[J]. Journal of China Pharmaceutical University, 2013, 44(6): 520-525. DOI: 10.11665/j.issn.1000-5048.20130606
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Drug-loaded micelles based on hyaluronic acid-paclitaxel prodrug: preparation and pharmacokinetic study in rats

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  • The aims of this study were to prepare high paclitaxel loading amount polymeric micelles based on amphiphilic hyaluronic acid-paclitaxel prodrug. The drug-loading and entrapment efficiency were characterized by HPLC. The physico-chemical properties of PTX loaded HA-PTX micelles(PTX-HA-PTX)was characterized by dynamic light scattering(DLS), transmission electron microscope(TEM), atomic force microscopy(AFM)and X-ray diffraction(XRD), respectively. The critical micelle concentration(CMC)was determined by fluorescence pyrene probe techniques. The pharmacokinetics behaviors of PTX-HA-PTX micelles were performed in rats taking Taxol as control. It was found that the drug-loading and encapsulation efficiency of PTX-HA-PTX micelles reached up to 41. 8% and 95. 4%, respectively. The nanoparticle size was approximately 213. 2 nm and the Zeta potential was -15. 5 mV. PTX-HA-PTX micelles exhibited to be almost spherical in shape from the observation by TEM and AFM. XRD results confirmed that PTX was either molecularly dispersed in the polymers or distributed in the micelles in an amorphous state. In pharmacokinetic studies, the AUC value of PTX-HA-PTX micelles increased significantly(P< 0. 01)while the CL value decreased significantly(P< 0. 05)compared with Taxol, which indicated slower clearance rate, prolonged residence time and enhanced therapeutic effect. All the results demonstrated that PTX-HA-PTX micelles might be a promising PTX delivery carrier for efficient tumor therapy.
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  • [1]
    Crown J,O′Leary M,Ooi WS.Docetaxel and paclitaxel in the treatment of breast cancer: a review of clinical experience[J].Oncologist,2004,9(Suppl 2):24-32.
    [2]
    Onetto N,Canetta R,Winograd B,et al.Overview of Taxol safety[J].J Natl Cancer Inst Monogr,1993(15):131-139.
    [3]
    Zhou J,Zhao WY,Ma X,et al.The anticancer efficacy of paclitaxel liposomes modified with mitochondrial targeting conjugate in resistant lung cancer[J].Biomaterials,2013,34(14):3 626-3 638.
    [4]
    Yang C,Wang J,Chen D,et al.Paclitaxel-Fe3O4 nanoparticles inhibit growth of CD138(-)CD34(-)tumor stem-like cells in multiple myeloma-bearing mice[J].Int J Nanomedicine,2013,8:1 439-1 449.
    [5]
    Takegami S,Takara K,Tanaka S,et al.Characterization,in vitro cytotoxicity and cellular accumulation of paclitaxel-loaded lipid nano-emulsions[J].J Microencapsul,2010,27(5):453-459.
    [6]
    Nornoo AO,Chow DS.Cremophor-free intravenous microemulsions for paclitaxel II.Stability,in vitro release and pharmacokinetics[J].Int J Pharm,2008,349(1/2):117-123.
    [7]
    Okamatsu A, Motoyama K, Onodera R, et al. Folate-appended beta-cyclodextrin as a promising tumor targeting carrier for antitumor drugs in vitro and in vivo[J].Bioconjug Chem,2013,24(4):724-733.
    [8]
    Pan Z,Gao Y,Heng L,et al.Amphiphilic N-(2,3-dihydroxypropyl)-chitosan-cholic acid micelles for paclitaxel delivery[J].Carbohydr Polym,2013,94(1):394-399.
    [9]
    Li J,Huo M,Wang J,et al.Redox-sensitive micelles self-assembled from amphiphilic hyaluronic acid-deoxycholic acid conjugates for targeted intracellular delivery of paclitaxel[J].Biomaterials,2012,33(7):2 310-2 320.
    [10]
    Zhang X,Lu J,Huang Y,et al.PEG-farnesylthiosalicylate conjugate as a nanomicellar carrier for delivery of paclitaxel[J].Bioconjug Chem,2013,24(3):464-472.
    [11]
    Ye Q,Huo MR,Zhou JP.Pharmacokinetics and in situ absorption in rat intestine of paclitaxel-loaded amphiphilic chitosan micelle[J].J China Pharm Univ(中国药科大学学报),2012,43(5):401-405.
    [12]
    Nam K,Nam HY,Kim PH,et al.Paclitaxel-conjugated PEG and arginine-grafted bioreducible poly(disulfide amine)micelles for co-delivery of drug and gene[J].Biomaterials,2012,33(32):8 122-8 130.
    [13]
    Wang Y,Xin D,Liu K,et al.Heparin-paclitaxel conjugates as drug delivery system: synthesis,self-assembly property,drug release,and antitumor activity[J].Bioconjug Chem,2009,20(12):2 214-2 221.
    [14]
    Zou AF,Huo MR,Zhou JP,et al.Octreotide-mediated N-octyl-O,N-carboxymethyl chitosan micelles:preparation,characterization and research as doxorubicin delivery vectors[J].J China Pharm Univ(中国药科大学学报),2011,42(2):124-130.
    [15]
    Gaucher G, Marchessault RH, Leroux JC. Polyester-based micelles and nanoparticles for the parenteral delivery of taxanes[J].J Control Release,2010,143(1):2-12.
    [16]
    Oh EJ,Park K,Kim KS,et al.Target specific and long-acting delivery of protein,peptide,and nucleotide therapeutics using hyaluronic acid derivatives[J].J Control Release,2010,141(1):2-12.
    [17]
    Zhang Y,Huo M,Zhou J,et al.PKSolver:an add-in program for pharmacokinetic and pharmacodynamic data analysis in microsoft excel[J].Comput Methods Programs Biomed,2010,99(3):306-314.
    [18]
    Huo M,Zhang Y,Zhou J,et al.Synthesis and characterization of low-toxic amphiphilic chitosan derivatives and their application as micelle carrier for antitumor drug[J].Int J Pharm,2010,394(1/2):162-173.
    [19]
    Stylianopoulos T.EPR-effect: utilizing size-dependent nanoparticle delivery to solid tumors[J].Ther Deliv,2013,4(4):421-423.
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