Coumarins in bamboo leaves of 10 species of Pleioblastus Nakai

12 coumarins in the leaves of 10 species of Pleioblastus Nakai were studied by HPLC. The 12 coumarins were coumarin, skimin, scopolin, scopoletin, umbelliferone, xanthotoxin, psoralen, pimpinellin, 5, 7-dimethoxycoumarin, 6, 7-dimethoxycoumarin, imperatorin and osthole. The 10 bamboo species included Pleioblastus amarus(Keng)Keng f. , P. simonii(Carr. )Nakai, P. maculatus(McClure)C. D. Chu et C. S. Chao, P. altiligulatus S. L. Chen et S. Y. Chen, P. yixingensis S. L. Chen et S. Y. Chen, P. amarus(Keng)Keng f. var. pendulifolius S. Y. Chen, P. solidus S. Y. Chen, P. juxianensis Wen, C. Y. Yao et S. Y. Chen, P. maculosoides Wen and P. amarus(Keng)Keng f. var. hangzhouensis S. L. Chen et S. Y. Chen. 6 coumarins including skimin, scopoletin, 6, 7-dimethoxycoumarin, umbelliferone, coumarin and pimpinellin were detected, and the contents of which were among the range of 2. 20-82. 20 mg/kg. P. simonii leaves were found to have the highest content of scopoletin. The results provide a reference for the comprehensive utilization of coumarins in the leaves of P. Nakai. This study is useful for the research on coumarins and chemical utilization of bamboo leave resources.