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JIANG Meixia, XIONG Hui, LI Yue, ZHOU Jianping, YAO Jing. Preparation and evaluation of thermo-sensitive hyaluronic acid/thiolated chitosan ophthalmic complex nano-hydrogel[J]. Journal of China Pharmaceutical University, 2014, 45(4): 413-419. DOI: 10.11665/j.issn.1000-5048.20140406
Citation: JIANG Meixia, XIONG Hui, LI Yue, ZHOU Jianping, YAO Jing. Preparation and evaluation of thermo-sensitive hyaluronic acid/thiolated chitosan ophthalmic complex nano-hydrogel[J]. Journal of China Pharmaceutical University, 2014, 45(4): 413-419. DOI: 10.11665/j.issn.1000-5048.20140406
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Preparation and evaluation of thermo-sensitive hyaluronic acid/thiolated chitosan ophthalmic complex nano-hydrogel

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  • The aim of this research was to prepare and evaluate thermo-sensitive complex nano-hydrogel(HP/CN)comprising of hyaluronic acid derivative(HA-PN)and thiolated chitosan(CMCS-NAC)for the ocular delivery of cyclosporine A(CsA). The CsA complex nano-hydrogel(CsA HP/CN)was prepared by incubation, then the size, Zeta potential, low critical solution temperature(LCST)and drug-loading capacity were characterized. The rabbit eye irritation effect, tissue adhesiveness and pharmacokinetic studies were investigated to evaluate the ocular compatibility and bioavailability of CsA HP/CN formulation. CsA HP/CN was found to be spherical in shape with a mean diameter of(122. 7±17. 8)nm, the Zeta potential of -(27. 87±2. 16)mV and LCST of 28. 4 °C with a drug-loading capacity up to 15. 08%. The rabbit eye irritation experiment showed no irritation on rabbit eyes. The CsA HP/CN group displayed significantly higher adhesive effect(1. 97- fold)on eye tissue as compared to the control group(drug carrier alone)after 15 min of ocular administration. Rabbit eyes pharmacokinetic data confirmed significantly higher AUC(1. 67- fold)and MRT(5. 17- fold)compared to the control group. Therefore, CsA HP/CN with small particle size and high drug loading capacity could obviously extend the drug retention time and increase its bioavailability on eyes, thus with potential application prospect in ocular drug delivery system.
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  • [1]
    El Tayar N,Mark AE,Vallat P,et al.Solvent-dependent conformation and hydrogen-bonding capacity of cyclosporin A:evidence from partition coefficients and molecular dynamics simulations[J].J Med Chem,1993,36(24):3 757-3 764.
    [2]
    Prasad V,Kumar N,Mishra PR.Amphiphilic gels as a potential carrier for topical drug delivery[J].Drug Deliv,2007,14(2):75-85.
    [3]
    Hornof MD,Kast CE,Bernkop-Schnürch A.In vitro evaluation of the viscoelastic properties of chitosan-thioglycolic acid conjugates[J].Eur J Pharm Biopharm,2003,55(2):185-190.
    [4]
    Kast CE,Bernkop-Schnürch A.Thiolated polymers—thiomers:development and in vitro evaluation of chitosan-thioglycolic acid conjugates[J].Biomaterials,2001,22(17):2 345-2 352.
    [5]
    Kast CE,Frick W,Losert U,et al.Chitosan-thioglycolic acid conjugate:a new scaffold material for tissue engineering[J].Int J Pharm,2003,256(1/2):183-189.
    [6]
    Schmitz T,Grabovac V,Palmberger TF,et al.Synthesis and characterization of a chitosan-N-acetyl cysteine conjugate[J].Int J Pharm,2008,347(1/2/3):79-85.
    [7]
    Kafedjiiski K,Hoffer M,Werle M,et al.Improved synthesis and in vitro characterization of chitosan-thioethylamidine conjugate[J].Biomaterials,2006,27(1):127-135.
    [8]
    Bernkop-Schnürch A,Hornof M,Zoidl T.Thiolated polymers—thiomers:synthesis and in vitro evaluation of chitosan-2-iminothiolane conjugates[J].Int J Pharm,2003,260(2):229-237.
    [9]
    Bernkop-Schnürch A, Kast C, Guggi D. Permeation enhancing polymers in oral delivery of hydrophilic macromolecules:thiomer/GSH systems[J].J Control Release,2003,93(2):95-103.
    [10]
    Kafedjiiski K,Föger F,Werle M,et al.Synthesis and in vitro evaluation of a novel chitosan-glutathione conjugate[J].Pharm Res,2005,22(9):1 480-1 488.
    [11]
    Leitner VM,Walker GF,Bernkop-Schnürch A.Thiolated polymers:evidence for the formation of disulphide bonds with mucus glycoproteins[J].Eur J Pharm Biopharm,2003,56(2):207-214.
    [12]
    Langoth N, Kalbe J, Bernkop-Schnürch A. Development of a mucoadhesive and permeation enhancing buccal delivery system for PACAP(pituitary adenylate cyclase-activating polypeptide)[J].Int J Pharm,2005,296(1/2):103-111.
    [13]
    Bernkop-Schnürch A,Pinter Y,Guggi D,et al.The use of thiolated polymers as carrier matrix in oral peptide delivery—proof of concept[J].J Control Release,2005,106(1/2):26-33.
    [14]
    Langoth N,Kahlbacher H,Schöffmann G,et al.Thiolated chitosans:design and in vivo evaluation of a mucoadhesive buccal peptide drug delivery system[J].Pharm Res,2006,23(3):573-579.
    [15]
    Clausen AE,Bernkop-Schnürch A.In vitro evaluation of the permeation-enhancing effect of thiolated polycarbophil[J].J Pharm Sci,2000,89(10):1 253-1 261.
    [16]
    Anitha A,Deepa N,Chennazhi K P,et al.Development of mucoadhesive thiolated chitosan nanoparticles for biomedical applications[J].Carbohydr Polym,2011,83(1):66-73.
    [17]
    Bernkop-Schnürch A,Hornof M,Guggi D.Thiolated chitosans[J].Eur J Pharm Biopharm,2004,57(1):9-17.
    [18]
    Wu Y,Yao J,Zhou J,et al.Enhanced and sustained topical ocular delivery of cyclosporine A in thermosensitive hyaluronic acid-based in situ forming microgels[J].Int J Nanomedicine,2013,8:3 587-3 601.
    [19]
    Zou Y,Brooks DE,Kizhakkedathu JN.A novel functional polymer with tunable LCST[J].Macromolecules,2008,41(14):5 393-5 405.
    [20]
    Langoth N, Kalbe J, Bernkop-Schnürch A. Development of a mucoadhesive and permeation enhancing buccal delivery system for PACAP(pituitary adenylate cyclase-activating polypeptide)[J].Int J Pharm,2005,296(1/2):103-111.
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