Simultaneous determination of six main compounds in<i> Nauclea officinalis</i> by ultra-performance liquid chromatography

CHEN Jiaquan; XU Guangfu; WANG Hui; WANG Jingjing; ZHU Fenxia

doi:10.11665/j.issn.1000-5048.20140409

Journal of China Pharmaceutical University > 2014 > 45(4): 434-437. > DOI: 10.11665/j.issn.1000-5048.20140409

CHEN Jiaquan, XU Guangfu, WANG Hui, WANG Jingjing, ZHU Fenxia. Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2014, 45(4): 434-437. DOI: 10.11665/j.issn.1000-5048.20140409

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Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography

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A UPLC method was established for the simultaneous determination of protocatechuic acid, chlorogenic acid, pumiloside, 3-epi-pumiloside, strictosamide and vincosamide in Nauclea officinalis. The experiment was performed with an Acquity BEH C₁₈(2. 1 mm ×100 mm, 1. 7 μm)column and the mobile phase consisting of acetonitrile and 0. 1% formic acid with gradient elution. The flow rate of 0. 4 mL/min and the injection volume of 2 μL as well as the column temperature of 40 ℃ were employed. Under the above-mentioned condition, 6 components in Nauclea officinalis were separated completely and the method was fully validated. Good linearity(R²> 0. 999 6), precision of inter- and intra-day(RSD≤4. 05%)together with average recoveries of 96. 59%-101. 8% were obtained. The method was proved to be rapid and accurate and can be used for the quality control of Nauclea officinalis.
- Nauclea officinalis,
- chemical constituents,
- determination of content,
- UPLC

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Simultaneous determination of six main compounds in Nauclea officinalis by ultra-performance liquid chromatography

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