Wogonin suppresses human alveolar adenocarcinoma cell A549 invasion and metastasis induced by IL-6

GAO Yuan; ZHAO Yue; YAO Jing; WANG Yu; GUO Qinglong; WU Yulin

doi:10.11665/j.issn.1000-5048.20140413

Journal of China Pharmaceutical University > 2014 > 45(4): 456-461. > DOI: 10.11665/j.issn.1000-5048.20140413

GAO Yuan, ZHAO Yue, YAO Jing, WANG Yu, GUO Qinglong, WU Yulin. Wogonin suppresses human alveolar adenocarcinoma cell A549 invasion and metastasis induced by IL-6[J]. Journal of China Pharmaceutical University, 2014, 45(4): 456-461. DOI: 10.11665/j.issn.1000-5048.20140413

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Wogonin suppresses human alveolar adenocarcinoma cell A549 invasion and metastasis induced by IL-6

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Abstract

To investigate the anti-metastasis effect of wogonin on human alveolar adenocarcinoma cell A549 induced by IL-6 and its potential mechanism, the growth inhibition effect of wogonin by MTT assay and the anti-metastasis effect of wogonin by transwell invasion assay were investigated. The immunofluorescence and Real-time PCR assay were employed to observe wogonin regulating metastasis marker protein E-cadherin, N-cadherin, Vimentin protein and mRNA level expression. Snail and Twist mRNA level expression and transcription factors of metastasis were also detected. In order to explore the potential mechanism of wogonin, PcDNA3. 1-Flag-STAT3 was transfected into A549 to detect metastasis marker protein mRNA level expression. Data showed that wogonin inhibited A549 cell invasion and metastasis, and regulated metastasis-related transcription factors and metastasis marker protein expression in a dose-dependent manner. In conclusion, all these results showed that wogonin can suppress A549 cells invasion andmetastasis induced by IL-6. It is possible that wogonin inhibits A549 cell invasion and metastasis by regulating the activity of transcription factor Snail and Twist.
- wogonin,
- A549 cells,
- invasion and metastasis,
- epithelial mesenchymal transition

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Wogonin suppresses human alveolar adenocarcinoma cell A549 invasion and metastasis induced by IL-6

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