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WANG Li, LI Mingdan, LU Yi, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells[J]. Journal of China Pharmaceutical University, 2014, 45(4): 469-474. DOI: 10.11665/j.issn.1000-5048.20140415
Citation: WANG Li, LI Mingdan, LU Yi, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells[J]. Journal of China Pharmaceutical University, 2014, 45(4): 469-474. DOI: 10.11665/j.issn.1000-5048.20140415
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Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells

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  • To evaluate the hepatotoxicity and its mechanism of the peptide-rich fraction(Fr-2)of the root of Aster tataricus in human liver L-02 cells. Cell viability was assessed by MTT assay and the contents of LDH, ROS, GSH, cytochrome C and Caspase-9/3 were assessed by commercially available kits. Cell apoptosis and mitochondrial membrane potential were measured by flow cytometry. The protein expressions of p-JNK, Bax, Bcl-2 were analysed by Western blot assay. The results demonstrated that the Fr-2 fraction induced apoptosis in a concentration- and time-dependent manner and provoked oxidative stress-associated inflammation in L-02 cells. It was characterized by loss of mitochondrial membrane potential and release of cytochrome c from mitochondria. The enhanced Bax/Bcl-2 ratio and the activated Caspase-9/3 indicated that the Fr-2 fraction induces apoptosis via mitochondria-dependent pathway in liver L-02 cells.
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