Simultaneous determination of four components in Xinkeshu capsules by HPLC and UPLC

The aim of the current study was to develop the methods for simultaneous determination of sodium danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin in Xinkeshu capsules by HPLC and UPLC. HPLC was performed on a Thermo C₁₈ column(4. 6 mm×250 mm, 5 μm)with a flow rate of 1. 0 mL/min. UPLC was performed on an Acquity UPLC T3 C₁₈ column(2. 1 mm×100 mm, 1. 8 μm)with a flow rate of 0. 5 mL/min. The mobile phases were both acetonitrile(A)-0. 1% trifluoroacetic acid solution(B)with gradient elution. The column temperatures were both 30 °C and the detection wavelengths were both set at 287 nm. Sodium danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin were separated from adjacent peaks completely. The linear relationships of calibration curves were favorable. The recoveries(%, n=9)of danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin were 100. 9, 99. 4, 98. 9, 101. 4(HPLC)and 100. 3, 100. 6, 99. 1, 100. 7(UPLC), respectively. The content of the two methods for 4 components was consistent, but the procedure of gradient elution of UPLC was simpler than that of HPLC.