Simultaneous determination of <i>p</i>-coumaric acid and ferulic acid in <i>Rhizama Phragmitis </i>by HPLC

PAN Chunyan; CHEN Jing; HANG Taijun; SONG Min

doi:10.11665/j.issn.1000-5048.20150214

Journal of China Pharmaceutical University > 2015 > 46(2): 219-223. > DOI: 10.11665/j.issn.1000-5048.20150214

PAN Chunyan, CHEN Jing, HANG Taijun, SONG Min. Simultaneous determination of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC[J]. Journal of China Pharmaceutical University, 2015, 46(2): 219-223. DOI: 10.11665/j.issn.1000-5048.20150214

Citation:

PDF (963 KB)

Simultaneous determination of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC

More Information

Graphical Abstract

Abstract

Abstract

To determine the contents of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC for quality evaluation. Rhizama Phragmitis alkaline alcoholic solution exact of p-coumaric acid and ferulic acid was refluxed. An HPLC method was developed by using a Diamonsil C₁₈(4. 6 mm×250 mm, 5 μm)column, and the mobile phase of acetonitrile and 0. 3% acetic acid with linear gradient elution at a flow of 1. 0 mL/min and UV detection at 310 nm. Calibration curvers of p-coumaric acid and ferulic acid were linear over the range of 0. 2-200 μg/mL and 0. 1-120 μg/mL respectively. . Average recoveries were(96. 9±2. 91)% and(98. 7±1. 78)%, respectively. The average contents of p-coumaric acid and ferulic acid expressed as mean±sd(max-min)were(0. 88±0. 16)%(1. 21%-0. 70%)and(0. 41±0. 035)%(0. 49%-0. 36%)in 15 batches of Rhizama Phragmitis. The established method will be beneficial to the quality evaluation of Rhizama Phragmitis.
- Rhizama Phragmitis,
- p-coumaric acid,
- ferulic acid,
- determination of content,
- HPLC

FullText(HTML)

References (24)

References

[1]	Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia: part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2010:152.
[2]	Chen JR,Li PL.Chinese traditional herbals in common use—Reed rhizome/Bamboo leaves/gypsum[J].J TCV M(中兽医医药杂志),2000(1):46-47.
[3]	Dai XP,Song HM,Li ZG.Determination of ferulic acid in different origins of Reed Rhizome by HPLC[J].TCM Res(中医研究),2010,23(9):26-27.
[4]	Chao RY,Yang JY,Cai XY,et al.Extraction,purification and anti-tumor activity of Reed Rhizome polysaccharide[J].Sci Tech Food Indus(食品工业科技),2011,32(12):284-286.
[5]	Zhang GS,Fan MY,Zhang JM,et al.Extraction and determination of Reed Rhizome polysaccharide[J].J Anhui TCM College(安徽中医学院学报),2002,21(1):51-52.
[6]	Geng ZH.Technique study of extracting polyose from Rhizome Phragmitis[J].Lishizhen Med Mater Med Res(时珍国医国药),1990,10(10):738.
[7]	Wang MF.Extraction,separation and determination of ferulic acid in Reed Rhizome[J].J Chizhou Teach College(池州师专学报),2007,21(3):51-52.
[8]	Yu WJ,Jiang TF,Wang YH,et al.Determination of the monosaccharide composition of polysaccharides from rhizome Phragmitis and pollen typhae by capillary zone electrophoresis[J].Trans Ocean Limn(海洋湖沼通报),2010,2:162-168.
[9]	Zhang GS,Li QR,Yin H,et al.Rapid determination of ferulic acid and identification of its structure in rhizome of Phragmitis communis by GC-TOF/MS[J].Chin Tradit Herb Drugs(中草药),2005,36(3):333-335.
[10]	Liu HY, Qiu NX, Ding HH, et al. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses[J].Food Res Int,2008(41):363-370.
[11]	Zhou JJ,Xie GR,Yan XJ.Traditional Chinese Medicines molecular Structures,Natural Sources and Applications(中药原植物化学成分手册)[M].Chemical Industry Press,2004:181.
[12]	Ou SY.Function and application of ferulic acid[J].Guangzhou Food Sci Tech(广州食品工业科技),2002,18(4):50-53.
[13]	Pragasam SJ,Murunikara V,Sabina EP,et al.Antiperoxidative potential of p-coumaric acid,a common dietary phenol,in adjuvant-induced arthritis in rats[J].Chin J Integr Med(中西医结合学报),2012,10(8):932-938.
[14]	Liu SS,Jin XQ,Gao D.Study on extraction methods of p-coumaric acid in Oldenlandia diffusa (willd.)roxb and determine the content by HPLC[J].Spcl Wild Eco Plant Res(特产研究),2006,1:21-24.
[15]	Yang GD,Liang MJ,He LC,et al.Solvent extraction methods of ferulic acid in Ligusticum Chuangxiong hort.[J].Chin Tradit Pat Med(中成药),2002,24(6):418-420.
[16]	Jin RC, Li GW, Ma SL, et al. Ferulic acid in Angelica sinensis extracting technology[J].Chin Tradit Pat Med(中成药),2008,30(4):516-519.
[17]	Zheng YM,Liu P.Determination of p-coumaric acid in Yanning capsule by HPLC[J].Tech Innov Appl(科技创新与应用),2014,12:29-30.
[18]	Yao GC,Zhao MJ,Pan YY,et al.Determination of p-coumaric acid in Houshuning tablets by HPLC[J].Chin J Chin Mater Med(中国中药杂志),2007,32(14):1470-1471.
[19]	Luo CY,Yin YL,Ruan Z,et al.Simultaneous determination of phenolic acids in potato tuber by RP-HPLC[J].Food Sci(食品科学),20011,32(18):300-303.
[20]	Luo F.Study of chemical constituents of rhizoma Phragmitis and the fingerprint of Ixeris sonchifolia(Bge.)(芦根的化学成分研究及苦碟子药材的指纹图谱研究)[D].Shenyang:Shenyang Pharmaceutical University,2008.
[21]	Mu HF,Li HJ,Chen J,et al.Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC[J].J China Pharm Univ(中国药科大学学报),2014,45(2):210-212.
[22]	Sun D,Liu WY,Liang C.Simultaneous quantitative determination of four active components in Salvia miltiorrhiza tablets by HPLC[J].J China Pharm Univ(中国药科大学学报),2007,38(1):51-54.
[23]	Chen MW,Xu DR,Wang ZT,et al.Quantitative determination of andrographolide and neoandrographolide in Andrographis paniculata Nees from different origrins and harvesting time by RP-HPLC[J].J China Pharm Univ(中国药科大学学报),1999,30(4):291-294.
[24]	Ding MY,Ma SW,Liu DL.Stability of ferulic acid and its existing form in Ligusticum chuanxiong and Angelica sinensis[J].Chin Tradit Herb Drugs(中草药),2004,35(1):28-30.

Cited By

Get Citation

PDF

XML

Article views (1241) PDF downloads (2277)

Turn off MathJax

Article Contents

Abstract

References

Simultaneous determination of p-coumaric acid and ferulic acid in Rhizama Phragmitis by HPLC

Abstract

References

Catalog

Export File

Citation

Format

Content