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GU Yi, LI Xiaoman, ZHENG Lufeng, YANG Jue, XI Tao. MicroRNA-10b regulates proliferation, cell cycle and apoptosis in human breast cancer cells[J]. Journal of China Pharmaceutical University, 2015, 46(2): 242-249. DOI: 10.11665/j.issn.1000-5048.20150218
Citation: GU Yi, LI Xiaoman, ZHENG Lufeng, YANG Jue, XI Tao. MicroRNA-10b regulates proliferation, cell cycle and apoptosis in human breast cancer cells[J]. Journal of China Pharmaceutical University, 2015, 46(2): 242-249. DOI: 10.11665/j.issn.1000-5048.20150218

MicroRNA-10b regulates proliferation, cell cycle and apoptosis in human breast cancer cells

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  • To investigate the effects of miRNA-10b(miR-10b)on breast cancer proliferation and apoptosis in MCF-7 and MDA-MB-231, the miR-10b mimics used to increase the endogenous expression of miR-10b, and miR-10b inhibitor used to decrease the endogenous expression of miR-10b, were stably transfected into MCF-7 and MDA-MB-231 breast cancer cells. The expression level of miR-10b was determined by real-time PCR. The effects of miR-10b on proliferation were evaluated by MTT assay, while cell cycle assay and the apoptosis rate were measured by flow cytometry. Bioinformatic software was used to predict the potential targets of miR-10b, and 3′UTR luciferase reporter and qRT-PCR assay were used to verify a direct target of miR-10b. The expression levels of caspase-3 and p21 protein were measured by Western blot. Results confirmed that over-expression of miR-10b could promote the proliferation of breast cancer cells and inhibit the apoptosis by up-regulating the endogenous miR-10b, while the miR-10b inhibitor could restrain the proliferation of breast cancer cells and increase the apoptosis by reducing the endogenous miR-10b. In conclusion, miR-10b could negatively regulate the expression of caspase-3 and p21 by targeting TP53INP1, hence highlighting its potential as an oncogene in breast cancer cells.
  • [1]
    Downs-Holmes C,SilvermanP.Breast cancer:overview&updates[J].Nurse Pract,2011,36(12):20-26.
    [2]
    Singer A,TresleyJ,Velazquez-Vega J,et al.Unusual aggressive breast cancer:metastatic malignant phyllodes tumor[J].J Radiol Case Rep,2013,7(3):24-37.
    [3]
    Liu J,Zheng M,Tang YL,et al.MicroRNAs,an active and versatile group in cancers[J].Int J Oral Sci,2011,3(4):165-175.
    [4]
    Andorfer CA,Necela BM,Thompson EA,et al.MicroRNA signatures:clinical biomarkers for the diagnosis and treatment of breast cancer[J].Trends Mol Med,2011,17(6):313-319.
    [5]
    Ladeiro Y, Couchy G. MicroRNA profiling in hepatocellular tumors is associated with clinical features and oncogene tumor suppressor gene mutations[J].Hepatology,2008,47(15):1955-1963.
    [6]
    Takashi S,Masamitsu N,Takeshi K,et al.MicroRNA-10b is overexpressed in malignant glioma and associated with tumor invasive factors uPAR and RhoC[J].Int J Cancer,2009,125(12):1407-1413.
    [7]
    Garzon R,Garofalo M.Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin[J].Proc Nat Acad Sci U S A,2008,105(10):3945-3950.
    [8]
    Ma L,Teruya-Feldstein J,Weinberg RA.Tumour invasion and metastasis initiated by microRNA-10b in breast cancer[J].Nature,2007,449(7163):682-688.
    [9]
    Okamura S, Arakawa H, Tanaka T, et al. p53DINP1, a p53-inducible gene,regulates p53-dependent apoptosis[J].Mol Cell,2001,8(1):85-94.
    [10]
    Tomasini R,Samir AA,Carrier A,et al.TP53INP1s and homeodomain-interacting protein kinase-2(HIPK2)are partners in regulating p53 activity[J].J Biol Chem,2003,278(39):37722-37729.
    [11]
    Le MT,Shyh-Chang N,Khaw SL,et al.Conserved regulation of p53 network dosage by microRNA-125b occurs through evolving miRNA-target gene pairs[J].PLoS Genet,2011,7(9):1-11.
    [12]
    Ito Y,Motoo Y,Yoshida H,et al.Decreased expression of tumor protein p53-induced nuclear protein 1(TP53INP1)in breast carcinoma[J].Anticancer Res,2006,26(6B):4391-4395.
    [13]
    Jiang PH,Motoo Y,Garcia S,et al.Down-expression of tumor protein p53-induced nuclear protein 1 in human gastric cancer[J].World J Gastroenterol,2006,12(5):691-696.
    [14]
    Gironella M,Seux M,Xie MJ,et al.Tumor protein p53-induced nuclear protein 1 expression is repressed by miR-155,and its restoration inhibits pancreatic tumor development[J].Proc Natl Acad Sci U S A,2007,104(41):16170-16175.
    [15]
    Bartel DP.MicroRNAs:target recognition and regulatory functions[J].Cell,2009,123(2):215-233.
    [16]
    Zhang B,Pan X,Cobb GP.MicroRNAs as oncogenes and tumor suppressors[J].Dev Biol,2007,302(28):1-12.
    [17]
    Negrini M,Calin GA.Breast cancer metastasis:a microRNA story[J].Breast Cancer Res,2008,10(2):303-307.
    [18]
    Yananadala V,Negoro H,Denker BM.Heterotrimeric G proteins and apoptosis:intersecting signaling pathways leading to context dependent phenotypes[J].Curr Mol Med,2009,9(5):527-545.
    [19]
    Seillier M,Peuget S,Gayet O,et al.TP53INP1,a tumor suppressor,interacts with LC3 and ATG8-family proteins through the LG3-interacting region(LIR)and promotes autophagy-dependent cell death[J].Cell Death Differ,2012,19(9):1525-1535.
    [20]
    Yeung ML,Yasunaga J,Bennasser Y,et al.Roles for micro RNAs,miR-93 and miR-130b,and tumor protein 53 induced nuclear protein 1 tumor suppressor in cell growth dysregulation by human T-cell lymphotrophic virus 1[J].Cancer Res,2008,68(21):8976-8985.
    [21]
    Ma S,Tang KH,Chan YP,et al.miR-130b promotes CD133(+)liver tumor-initiating cell growth and self-renewal via tumor protein 53-induced nuclear protein 1[J].Cell Stem Cell,2010,7(6):694-707.
    [22]
    Jiang F,Liu T,He Y,et al.MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo[J].BMC Cancer,2011,5(11):425-437.
    [23]
    Shibuya H,Linuma H,Shimada R,et al.Clinic opathological and prognostic value of microRNA-21 and microRNA-155 in colorectal cancer[J].Oncology,2010,79(3/4):313-320.
    [24]
    Yamamoto Y, Yoshioka Y, Minoura K, et al. An integrative genomic analysis revealed the relevance of microRNA and gene expression for drug-resistance in human breast cancer cells[J].Mol Cancer,2011,10(1):135-151.
    [25]
    Salmena L,Poliseno L,Tay Y,et al.A ceRNA hypothesis:the Rosetta Stone of a hidden RNA language[J]?Cell,2011,146(3):353-358.
    [26]
    Tay Y,Kats L,Salmena L,et al.Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs[J].Cell,2011,147(2):344-357.
    [27]
    Sumazin P,Yang X,Chiu HS,et al.An extensive microRNA-mediated network of RNA-RNA interactions regulates established oncogenic pathways in glioblastoma[J].Cell,2011,147(2):370-381.

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