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ZHANG Zhiqing, WANG Fang, ZHOU Ting, ZHANG Guodong, WANG Xiufeng, LI Yunze. A DNA polyaptamer system as a targeted antitumor drug delivery[J]. Journal of China Pharmaceutical University, 2015, 46(4). DOI: 10.11665/j.issn.1000-5048.20150408
Citation: ZHANG Zhiqing, WANG Fang, ZHOU Ting, ZHANG Guodong, WANG Xiufeng, LI Yunze. A DNA polyaptamer system as a targeted antitumor drug delivery[J]. Journal of China Pharmaceutical University, 2015, 46(4). DOI: 10.11665/j.issn.1000-5048.20150408

A DNA polyaptamer system as a targeted antitumor drug delivery

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  • A long chain structure of DNA(polyaptamer)composed of multiple aptamer units was synthesized by rolling circle amplification and used for the construction of polyaptamer-doxorubicin system in the treatment of leukemia cells. It was found that the system was significantly more effective than monoaptamer in targeting and killing leukemia cells as it provided 35-fold enhanced binding affinity and 10-fold greater drug loading via multivalent effects. Drug release and cell viability also proved that the conjugates could gain entrance into the cells and rapidly release doxorubicin under the action of lysosome, leading to the tumoricidal effect.
  • [1]
    Wang J,Chen C.Present status of individualized therapy in childhood acute lymphoblastic leukemia[J].J Exp Hematol(中国实验血液学杂志),2013,21(6):1617-1622.
    [2]
    Peer D,Karp JM,Hong S,et al.Nanocarriers as an emerging platform for cancer therapy[J]. Nat Nanotechnol,2007, 2(12):751-760.
    [3]
    Hubbell JA,Chilkoti A. Nanomaterials for drug delivery[J]. Science,2012, 337(6092):303-305.
    [4]
    Mammen M,Choi SK,Whitesides GM.Polyvalent interactions in biological systems:implications for design and use of multivalent ligands and inhibitors[J]. Angew Chem Int Ed,1998, 37(20):2754-2794.
    [5]
    Kolonko EM,Kiessling LL.A polymeric domain that promotes cellular internalization[J].J Am Chem Soc,2008,130(17):5626-5627.
    [6]
    Tong GJ,Hsiao SC,Carrico ZM,et al.Viral capsid DNA aptamer conjugates as multivalent cell-targeting vehicles[J].J Am Chem Soc,2009,131(31):11174-11178.
    [7]
    Yang L,Meng L,Zhang X,et al.Engineering polymeric aptamers for selective cytotoxicity[J].J Am Chem Soc,2011,133(34):13380-13386.
    [8]
    Amiram M,Quiroz FG,Callahan DJ,et al.A highly parallel method for synthesizing DNA repeats enables the discovery of ‘smart’ proteinpolymers[J].Nat Mater,2011,10(2):141-148.
    [9]
    Lee JB,Peng S,Yang D,et al.A mechanical metamaterial made from a DNA hydrogel[J].Nat Nanotechnol,2012,7(12):816-820.
    [10]
    Zhao W,Brook MA,Li Y.Periodic assembly of nanospecies on repetitive DNA sequences generated on gold nanoparticles by rolling circle amplification[J]. Methods Mol Biol,2008, 474:79-90.
    [11]
    Shangguan D,Li Y,Tang Z,et al.Aptamers evolved from live cells as effective molecular probes for cancer study[J].Proc Natl Acad Sci U S A,2006,103(32):11838-11843.

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