Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9

LI Guojun; ZHAO Kai; YAO Yuyuan; SHEN Le; GUO Qinglong

doi:10.11665/j.issn.1000-5048.20150413

Journal of China Pharmaceutical University > 2015 > 46(4): 464-468. > DOI: 10.11665/j.issn.1000-5048.20150413

LI Guojun, ZHAO Kai, YAO Yuyuan, SHEN Le, GUO Qinglong. Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9[J]. Journal of China Pharmaceutical University, 2015, 46(4): 464-468. DOI: 10.11665/j.issn.1000-5048.20150413

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Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9

LI Guojun^1,2,
ZHAO Kai^1,2,
YAO Yuyuan^1,2,
SHEN Le^1,2,
GUO Qinglong^1,2

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Abstract

Abstract

To investigate the apoptotic effect of flavonoid compound GL-V9 on human gastric cancer cells and its potential mechanism, MGC-803 and BGC-823 cells were treated with GL-V9. MTT assay was performed to assess the growth inhibition effects on MGC-803 and BGC-823 cells under different concentrations of GL-V9. Annexin V-FITC/PI staining assay was employed to observe the apoptotic rate of GL-V9 cells with the treatment of GL-V9. DAPI staining was performed to observe the nuclear morphological changes using fluorescence microscopy. Activation of caspase-9 and caspase-3 was analyzed by Western blotting. Ca²⁺ concentration in gastric cancer cells was detected by Fluo-3 AM staining assay. Results showed that GL-V9 could inhibitcell viability, change the nuclear morphologyl, activate caspase-9 and caspase-3 and induce the apoptosis in gastric cancer cells. The mechanism of the induction of apoptosis in MGC-803 cells under the treatment of GL-V9 may aetivate the Ca²⁺ associated mitochondrial apoptosis pathway.
- flavonoid compound,
- GL-V9,
- gastric adenocarcinoma,
- MGC-803 cell,
- apoptosis,
- Ca²⁺

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Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9

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