Preparation of an electrochemical immunosensor modified with graphene for detection of dienestrol in pork

An ultrasensitive electrochemical immunosensor modified with graphene and dienestrol(DE)was developed for the detection of dienestrol through indirect competition with K₃Fe(CN)₆ acting as the redox probes. The results revealed that under optimized conditions a calibration for DE was obtained with a linear range of 500-5 000 ng/mL and the detection limit was up to 0. 2 ng/mL, showing that the proposed electrochemical immunosensor had excellent sensitivity and wide detection range. The immunosensor was examined in real samples for the analysis of DE. A good recovery in the range of 83. 8%-97. 7% was obtained in pork samples.