Advanced Search
ZHOU Yijie, DOU Rongkun, BI Zhenfei, YANG Yalan, LIU Zongying, WU Yiran, QIU Jinyi, XIANGFEI Danzhou, MAO Canquan. Promotion of apoptosis in leukemia K562 cells by natural plant-derived antimicrobial solution(PAMs)[J]. Journal of China Pharmaceutical University, 2015, 46(6): 712-718. DOI: 10.11665/j.issn.1000-5048.20150613
Citation: ZHOU Yijie, DOU Rongkun, BI Zhenfei, YANG Yalan, LIU Zongying, WU Yiran, QIU Jinyi, XIANGFEI Danzhou, MAO Canquan. Promotion of apoptosis in leukemia K562 cells by natural plant-derived antimicrobial solution(PAMs)[J]. Journal of China Pharmaceutical University, 2015, 46(6): 712-718. DOI: 10.11665/j.issn.1000-5048.20150613

Promotion of apoptosis in leukemia K562 cells by natural plant-derived antimicrobial solution(PAMs)

More Information
  • In this study, the leukemia K562 cell line was used as a model to elucidate the anticancer effects and preliminary mechanisms of PAMs. MTT assay showed that PAMs could cause cytotoxicities in K562 cells in dose- and time-dependent manners. AO-EB, Annexin-FITC/PI staining showed that the killing effects of PAMs in K562 cells were related to apoptosis, which was further confirmed by the following molecular and enzymatic assay. The mRNA levels of pro-apoptotic genes caspase-3, caspase-9 and bax were remarkably increased while the anti-apoptotic gene bcl-2 was significantly decreased determined by fluorescent quantitative PCR. Western blotting disclosed that PAMs could up-regulate caspase-3 and down-regulate anti-apoptotic survivin protein expression. The latter was also consistent with the results that PAMs could increase the enzymatic activities of both caspase-3 and caspase-9. All these results suggested that PAMs could effectively inhibit the proliferation of K562 cells and the mechanisms may be closely related to apoptosis induction. The work provides evidence basis for PAMs to be potentially developed as anti-cancer leukemia Chinese medicine.
  • [1]
    Chopra M,Bohlander SK.Disturbing the histone code in leukemia:translocations and mutations affecting histone methyl transferases[J].Cancer Genetics,2015,208(5):192-205.
    [2]
    Sun XD,Cai YH,Cao YF,et al.Investigation of the dangerous factors of child leukemia[J].Med J CASC(中国航天医学杂志),2003,12(5):1-3.
    [3]
    Asmaa MJ,Al-Jamal HA,Ang CY,et al.Apoptosis induction in MV4-11 and K562 human leukemic cells by Pereskia sacharosa (Cactaceae)leaf crude extract[J].Asian Pac J Cancer Prev,2014,15(1):475-481.
    [4]
    Li RF, Feng YQ, Chen JH, et al. Naringenin suppresses K562 human leukemia cell proliferation and ameliorates adriamycin-induced oxidative damage in polymorphonuclear leukocytes[J].Exp Ther Med,2015,9(3):697-706.
    [5]
    Schürch CM, Riether C, Ochsenbein AF. Dendritic cell-based immunotherapy for myeloid[J].Front Immunol,2013,4(496):1-16.
    [6]
    Xu GJ,Wang Q,Yu BY,et al.Color map of Chinese herbal medicine agains tumor(抗肿瘤中草药彩色图谱)[M].Fuzhou:Fujian Science and Technology Press,2000:368.
    [7]
    Huang H,Liu ZC.Antitum or effect of shikonin and its derivatives[J].Chin J Cancer Prev Treat(中华肿瘤防治杂志),2005,12(1):75-78.
    [8]
    Sun J,Zhang C,Bao YL,et al.Parthenolide-induced apoptosis,autophagy and suppression of proliferation in HepG2 cells[J].Asian Pac J Cancer Prev,2014,15(12):4897-4902.
    [9]
    Tsujimoto Y,Finger LR,Yunis J,et al.Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18)chromosome translocation[J].Science,1984, 226(4678):1097-1099.
    [10]
    Apte SS,Mattei MG,Olsen BR.Mapping of the human BAX gene to chromosome 19q13.3-q13.4 and isolation of a novel alternatively spliced transcript,BAX delta.Genomics[J].Genomics,1995,26(3):592-594.
    [11]
    Ouyang L,Shi Z,Zhao S,et al.Programmed cell death pathways in cancer:a review of apoptosis,autophagy and programmed necrosis[J].Cell Prolif,2012,45(6):487-498.
    [12]
    Hu Y,Benedict MA,Ding L,et al.Role of cytochromec and dATP/ ATP hydrolysis in Apaf-1-mediated caspase 9 activation and apoptosis[J].EMBO J,1999,18(13):3586-3595.
    [13]
    Ambrosini G,Adida C,Altieri DC.A novel anti-apoptosis gene,survivin,expressed in cancer and lymphoma[J].Nat Med,1997,3(8):917-921.
    [14]
    Pennati M,Binda M,Colella G,et al.Ribozyme-mediated inhibition of survivin expression increases spontaneous and drug-induced apoptosis and decreases the tumorigenic potential of human prostate cancer cells[J].Oncogene,2004,23(2):386-394.
  • Related Articles

    [1]LIU Yuanyuan, HE Chao. Preparation,optimization and antibacterial activity of luteolin nanostructured lipid carriers[J]. Journal of China Pharmaceutical University, 2020, 51(6): 681-687. DOI: 10.11665/j.issn.1000-5048.20200606
    [2]GAO Liuzhou, XIE Yusuo, HUANG Wenlong, HU Guoqiang. Synthesis, antibacterial and antitumor activities of 1-cycloproyl-6-fluoro-7-(hydrazone)-quinolin-4(1H)-one-carboxylic acids[J]. Journal of China Pharmaceutical University, 2014, 45(6): 662-664. DOI: 10.11665/j.issn.1000-5048.20140607
    [3]HU Guo-qiang, HOU Li-li, WANG Guo-qiang, DUAN Nan-nan, WEN Xiao-yi, CAO Tie-yao, HUANG Wen-long. Synthesis and antitumor and antibacterial activities of fluoroquinolone C-3 isosteres I.norfloxacin C-3 carbonylhydrazone derivatives[J]. Journal of China Pharmaceutical University, 2012, 43(4): 298-301.
    [4]CHEN Guo-hua, REN Zhong, YANG Yang, WU Fei-hua. Synthesis and antibacterial activity of novel fourth-generation cephalosporin compounds[J]. Journal of China Pharmaceutical University, 2009, 40(5): 395-399.
    [6]In vitro and In vivo Antibacterial Activities of Fleroxacin Injection[J]. Journal of China Pharmaceutical University, 1997, (5): 53-57.
    [7]Synthesis and Antimicrobial Activity of Triazolylcepha-losporins[J]. Journal of China Pharmaceutical University, 1993, (6): 321-326.
    [8]Preparation and Antibacterical Activity of Four New Complexes Between La, Pr, Nd or Sm and Lomefloxacin[J]. Journal of China Pharmaceutical University, 1993, (5): 308-310.
    [9]Synthesis and Antibacterial Activity of 6, 8-Difluoro Quinolones[J]. Journal of China Pharmaceutical University, 1993, (5): 264-268.
    [10]Antimicrobial Activity (in Vitro) of the Constituents of Bulbus Fritillariae[J]. Journal of China Pharmaceutical University, 1992, (3): 188-189.
  • Cited by

    Periodical cited type(8)

    1. 龚蕾蕾. 纳米药物递送系统治疗乳腺癌的研究进展. 医学理论与实践. 2023(06): 939-941 .
    2. 卓新雨,张艾立,马菲,崔志磊,刘臻,谢恬. 纳米载药系统的研究进展. 广东化工. 2022(10): 85-87 .
    3. 刘盼,王路,董能峰,刘力,李炳生. 量子点的制备及其在药物检测和药物递送领域中的应用. 广东化工. 2021(08): 152-153+151 .
    4. 文鹏,石峰. 纳米材料在肿瘤治疗中的研究进展. 肿瘤药学. 2021(02): 153-157+164 .
    5. 程晓昆,张越,吕海军,刘歆颖,侯森林,陈爱兵. 多孔碳纳米材料构建抗肿瘤药物靶向传递系统的研究进展. 无机材料学报. 2021(01): 9-24 .
    6. 王小宁,闫梦茹,马远涛,梁晓燕,罗国平. 载紫杉醇聚(2-乙基-2-噁唑啉)修饰单壁碳纳米管递药系统的制备及体外抗肿瘤作用评价. 中草药. 2020(03): 607-615 .
    7. 王鹏. 基于碳纳米线圈的细胞应激性测试探针. 云南大学学报(自然科学版). 2020(05): 941-948 .
    8. 李学暖,李天昊,张嘉琳,黎孔月,蒋靖超,程昊. 多壁碳纳米管修饰玻碳电极电致化学发光测奈福泮. 广西科技大学学报. 2020(04): 124-131 .

    Other cited types(8)

Catalog

    Article views (1453) PDF downloads (2068) Cited by(16)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return