Culture process for fully human anti-VEGF165 monoclonal antibody

This study was focus on the optimizing cell culture process of recombinant DG44 cells which expressing novel fully human anti-VEGF165 monoclonal antibody(HVAB). Feed-batch culture and two-phase temperature control were studied for optimizing the HVAB productivity in shaken flasks. The influences of pH changes were determined to study the growth of DG44 cell and expression of HVAB in bioreactors. The HVAB productivity was rose from 101 mg/L to 654 mg/L after feed-batch culture in shaken flask, and cell viability maintained above 80% after reduce the temperature in middle growth phase. It is also suggested that pH range at 6. 4-7. 4 is beneficial to DG44 cells growth and HVAB expression. The productivity in bioreactor is 526 mg/L decreased 11% compare with in shaken flask, which laid an foundation for further pilot scale production.