Influence of ubiquitous chromatin opening element on the antibody expression in CHO cells

Ubiquitous chromatin opening element(UCOE), composed of the promoters of human housekeeping genes, prevents transgene from silencing and produces consistent, stable and high-level gene expression irrespective of the chromosomal integration site. The research studied the influence of different UCOE element parts on antibody expression in CHO cells. UCOE 1. 5 kb from chromobox homolog 3(CBX3), UCOE 2. 5 kb from the heterogeneous ribonucleoprotein A2/B1(HNRPA2B1)and the whole UCOE 4. 0 kb were inserted into the antibody light and heavy chain vectors, respectively, and transfected into CHO cells using antibiotics-Zeocin and Blasticidin for pressure selection. Four groups of monoclonal cells were harvested and antibody expression of each group was detected. The monoclonal cells with UCOE 1. 5 kb and UCOE 2. 5 kb increased 1. 5 to 2-fold in the level of antibody expression, wheareas, monoclonal cells with UCOE 4. 0 kb increased 3 to 4-fold. The enhancement of two housekeeping promoter genes on antibody expression could stack up.