• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
CHENG Ziting, YAO Ke, LIU Wanwan, XU Bohui, ZHU Hongyan. Preparation and characterization of composite delivery system of paclitaxel-loaded temperature sensitive liposome and siRNA-loaded gold nanostar[J]. Journal of China Pharmaceutical University, 2017, 48(4): 445-452. DOI: 10.11665/j.issn.1000-5048.20170409
Citation: CHENG Ziting, YAO Ke, LIU Wanwan, XU Bohui, ZHU Hongyan. Preparation and characterization of composite delivery system of paclitaxel-loaded temperature sensitive liposome and siRNA-loaded gold nanostar[J]. Journal of China Pharmaceutical University, 2017, 48(4): 445-452. DOI: 10.11665/j.issn.1000-5048.20170409
shu

Preparation and characterization of composite delivery system of paclitaxel-loaded temperature sensitive liposome and siRNA-loaded gold nanostar

More Information
    Graphical Abstract
    • Abstract
  • We prepared gold nanostar(GNS)through seed growth method firstly, then formation of COX-2 siRNA(siCOX-2)and GNS composite modified with polyethylene glyco(PEG), 2-amino-2-deoxy-D-glucos(DG)and 9-D-arginin(9R)was prepared. Afterwords, paclitaxel temperature sensitive liposome(PTX-TSL)was prepared by film dispersion method. Finally, siCOX-2 delivery systerm(PTX-TSL-(siCOX-2(9R/DG-GNS)))was obtained by hydrosulfuryl ligand reaction between siCOX-2(9R/DG-GNS)and PTX-TSL. The successful build of siCOX-2(9R/DG-GNS)was vetified by nuclear magnetic resonance(NMR), sodium dodecyl sulfate polyacryl amide gel electrophoresis(SDS-PAGE), and ultraviolet spectrophotometry and agarose gel electrophoresis method. Particle size of PTX-TSL-(siCOX-2(9R/DG-GNS))was(292±14)nm and Zeta potential was about -(2. 59±0. 12)mV, which were measured by Zetasizer Nano ZS90. The morphology of PTX-TSL-(siCOX-2(9R/DG-GNS))measured by transmissionelectronmicroscopy showed homogeneous star structure with phospholipid bilayer on the surface, and it showed good thermal conversion efficiency under radiation of 808 nm laser. Differential scanning calorimetry test showed that PTX-TSL phase transition temperature is about 42. 6 °C. The drug loading content(using dialysis method)and encapsulation efficiency of PTX-TSL were about 7. 5% and 95. 4%, at the same time, the release process experiment of PTX-TSL showed that it had a good temperature sensitive release performance. It is hopeful that this siCOX-2 system can be used for reducing drug resistance of PTX and improving the treatment effect of PTX through the synergistic antitumor drug resistance effect of siCOX-2.
    • FullText(HTML)
    • References (13)
  • [1]
    Guojia L.Effect of glycolytic inhibitors on growth and apoptosis of osteosarcoma cells under hypoxia condition[D].Huazhong University of Science and Technology(华中科技大学):2008.
    [2]
    Penson RT, Oliva E, Skates SJ, et al. Expression of multidrug resistance-1 protein inversely correlates with paclitaxel response and survival in ovarian cancer patients:a study in serial samples[J],Gynecol Oncol,2004,93(1):98-106.
    [3]
    Orr GA,Verdier-Pinard P,McDaid H,et al.Mechanisms of taxol resistance related to microtubules[J].Oncogene,2003,22(47):7280-7295.
    [4]
    Tabuchi Y,Matsuoka J,Gunduz M,et al.Resistance to paclitaxel therapy is related with Bcl-2 expression through an estrogen receptor mediated pathway in breast cancer[J].Int J Oncol,2009,34(2):313-319.
    [5]
    Agaiwal R, Kaye SB. Ovarian cancer strategies for overcoming resistance to chemotherapy[J].Nat Rev Cancer,2003,3(7):502-516.
    [6]
    Hortobagyi GN,Ueno NT,Xia W,et al.Cationic liposome mediated E1A gene trensfer to human breast and ovarian cancer cells and its biologic effects a phase I clinical trial[J].Clin Oncol,2001,19(14):3422-3433.
    [7]
    Garheng Kong,Rod D.Braun,Mark W.Hyperthermia enables tumor-specific nanoparticle delivery:effect of particle size[J].Cancer Res,2000,60(16):4440-4445.
    [8]
    Raspollini MR,Amunni G,Villanucci A,et al.Increased cyclooxygenase-2(COX-2)and P-glycoprotein-170(MDR1)expression is associated with chemotherapy resistance and poor prognosis.Analysis in ovarian carcinoma patients with low and high survival[J].Int J Gynecol Cancer,2005,15(2):255-260.
    [9]
    Sheng H,Shao J,Morrow JD,et al.Modulation of apoptosis and Bcl-2 expression by prostagland in E2 inhuman colon cancer cells[J].Cancer Res,1998,58(3):362-366.
    [10]
    Oshima M,Dinchuk JE,Kargman SL,et al.Suppression of intestinal polyposis in Apcdelta716 knockout mice by inhibition of cyclooxygenase 2(COX-2)[J].Cell,1996,87(5):803-809.
    [11]
    Zhu H,Zhang S,Ling Y,et al.pH-responsive hybrid quantum dots for targeting hypoxic tumor siRNA delivery[J].J Control Release,2015,220(Pt A):529-544.
    [12]
    Giacomelli C,Schmidt V,Borsali R.Nanocontainers formed by self-assembly ofpoly(ethylene oxide)-b-poly(glycerol monomethacrylate)-drug conjugates[J].Macromolecules,2014,40(6):2148-2157.
    [13]
    Morita T,Horikiri Y,Suzuki T,et al.Preparation of gelatin microparticles by co-lyophilization with poly(ethylene glycol):characterization and application to entrapment into biodegradable microspheres[J].Int J Pharm,2001,219(1/2):127-137.
    • Related Articles

  • Related Articles

    [1]SHUAI Yubing, WANG Qiudan, HE Tianyu, CAO Lijuan. Mitofusin 2: an emerging drug target[J]. Journal of China Pharmaceutical University, 2025, 56(1): 1-9. DOI: 10.11665/j.issn.1000-5048.2024041001
    [2]CHEN Haitao, LU Xiaofei, HAN Fujiao, WANG Wenxin, FU Jingguo, CHEN Hui. Asymmetric hydrogenation of methyl-2-acylamino-3-arylacrylate[J]. Journal of China Pharmaceutical University, 2018, 49(5): 553-557. DOI: 10.11665/j.issn.1000-5048.20180506
    [3]QIAO Jianan, WANG Tingfang, ZHANG Can. Design, synthesis and bioactivities of 4-(3-sulfonylbenzene)amino-6-formylpyrrole［2, 3-d］ pyrimidine derivatives[J]. Journal of China Pharmaceutical University, 2017, 48(5): 554-562. DOI: 10.11665/j.issn.1000-5048.20170508
    [4]CHEN Haitao, DUAN Xianying, WANG Wenxin, FU Jingguo, YANG Guanyu, LI Ji. Synthesis of(Z)-methyl-2-acetamidocinnamate[J]. Journal of China Pharmaceutical University, 2015, 46(2): 178-180. DOI: 10.11665/j.issn.1000-5048.20150207
    [5]Effect of N-[4-[2-[acetyl[2-naphthyloxy]ethyl]amino]-ethyl]phenylmethansulfonamide on Isolated Guinea Pig Atria[J]. Journal of China Pharmaceutical University, 2002, (4): 69-72.
    [6]Improved Synthesis of 2-(3-benzoylphenyl) propionitrile[J]. Journal of China Pharmaceutical University, 2001, (3): 27-28.
    [7]Ni Peizhou, Lu Jian, Tang Weifang, Zhou Ping, Xia Lin. Synthesis of 2 (2 Methoxyphenoxy)ethylamine[J]. Journal of China Pharmaceutical University, 1996, (6).
    [8]Pharmacological Effects of IL-2[J]. Journal of China Pharmaceutical University, 1992, (5): 313-315.
    [9]Synthesis of the Derivatives of 2-Acetylbenzimidazolyl Thiosemicarbazone[J]. Journal of China Pharmaceutical University, 1990, (5): 295-297.
    [10]SYNTHESIS OF 2-(2-CHLOROACETAMIDOTHIAYOL-4-YL)-(Z)-2-METHOXYIMINO ACETIC ACID[J]. Journal of China Pharmaceutical University, 1985, (2): 4-6.

Catalog

    Get Citation
    PDF
    XML
    Article views (1185) PDF downloads (2035) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：365498

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return