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HU Lixin. RP-HPLC determination of two stereoisomers in latanoprost eye drop[J]. Journal of China Pharmaceutical University, 2017, 48(5): 596-600. DOI: 10.11665/j.issn.1000-5048.20170515
Citation: HU Lixin. RP-HPLC determination of two stereoisomers in latanoprost eye drop[J]. Journal of China Pharmaceutical University, 2017, 48(5): 596-600. DOI: 10.11665/j.issn.1000-5048.20170515
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RP-HPLC determination of two stereoisomers in latanoprost eye drop

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  • To establish an HPLC method for the determination of impurity I and impurity II in latanoprost eye drop. HPLC separation was carried on a Zorbax SB- C18 column(4. 6 mm250 mm, 5 m)with the mobile phase consisting of methanol-acetonitrile-water(adjusting pH to 3. 0 with acetic acid)(56 ∶14 ∶30). The flow rate was 1. 0 mL/min; the detection wavelength was 210 nm; and the injection volume was 100 μL. Impurity I, impurity II and latanoprost were separated efficiently under the selected HPLC conditions, with good linearity in the range of 0. 049 99-0. 999 8 g/mL for impurity I and 0. 049 94-0. 998 8 g/mL for impurity II. The average recoveries of impurity I and II were 96. 74%(n=9)and 94. 99%(n=9), respectively. The established method can be used for the detection of stereoisomers in latanoprost eye drop.
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  • [1]
    Allaire C,Dietrich A,Allemier H,et al.Latanoprost 0.005% test formulation is as effective as Xalatan in patients with ocular hypertension and primary open-angle glaucoma[J]. Eur J Ophthalmol,2012,22(1):19-25.
    [2]
    Zanoni G,D′ Alfonso A,Porta A,et al.The meyere-schuster rearrangement:a new synthetic strategy leading to prostaglandins and their drug analogs,bimatoprost and latanoprost[J].Tetrahedron,2010,66(38):7472-7477.
    [3]
    Martynow JG, Jozwik J, Szelejewski W, et al. A new synthetic approach to high-purity(15R)-latanoprost[J].Eur J Org Chem,2007,2007(4):689-695.
    [4]
    Xiu ZM,Wang LC,Huang MY,et al.A novel method for the synthesis of latanoprost[J]. Chin J Org Chem(有机化学),2014,34:1786-1791.
    [5]
    Widomski P,Baran P,Golebiewski P,et al.Validated liquid chromatographic method for analysis of the isomers of latanoprost[J].Acta Chromatographica,2008,20(2):157-164.
    [6]
    Pan DJ,Chen QF,Zhang JD,et al.Determination of the related substances in latanoprost eye drops by NP-HPLC[J].Chin J Pharm(中国医药工业杂志),2013,44(9):932-934.
    [7]
    Chinese Pharmacopoeia Commision.Chinese Pharmacopoeia:part 4(中华人民共和国药典:四部)[S].Beijing:China Medical Science Press.2015:374.
    [8]
    Zhang L.Qualitative and quantitative analysis for determination of related substances in piracetam raw materials and its injection[J]. Chin J Pharm Anal(药物分析),2014,34(6):1062-1066.
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