Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD

HUI Renjie; HE Xu; LIU Jingyi; FENG Jing; ZENG Shuai; FENG Bainian

doi:10.11665/j.issn.1000-5048.20170610

Journal of China Pharmaceutical University > 2017 > 48(6): 696-700. > DOI: 10.11665/j.issn.1000-5048.20170610

HUI Renjie, HE Xu, LIU Jingyi, FENG Jing, ZENG Shuai, FENG Bainian. Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD[J]. Journal of China Pharmaceutical University, 2017, 48(6): 696-700. DOI: 10.11665/j.issn.1000-5048.20170610

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Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD

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Abstract

To establish a rapid determination method of six flavonoids: catechin, rutin, myricetin, quercetin, kaempferol and isorhamnetin, from seabuckthorn leaves by RP-HPLC-DAD. The seabuckthorn leaves were first degreased by petroleum ether, extracted by ethanol, and determined by RP-HPLC-DAD. The six flavonoids were separated and eluted by a Shimadzu C₁₈(150 mm×2. 1 mm, 5 μm)column with methanol-water(0. 1% phosphoric acid)(60∶40)at a flow rate of 1. 0 mL/min. The detection wavelength were as follow: catechin 208 nm, rutin 257 nm, myricetin 373 nm, quercetin 371 nm, kaempferol 367 nm, and isorhamnetin 371 nm, respectively. The injection volume was 20 μL. The contents of the six flavonoids were in the range of 0. 47 to 30. 00 μg/mL with good linearity. The validation of the method, including precision, stability and recovery rate, was acceptable. The established method can be used for fast determination of the content of six flavonoids in seabuckthorn leaves.
- seabuckthorn leaves,
- RP-HPLC-DAD,
- flavonoids,
- rapid determination

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[1]	Liu R,Zhang C.Advances in the research of the chemical components of seabuckthorn[J].J Shanxi Datong Univ(Nat Sci Edition)[山西大同大学学报(自然科学版)],2009,25(2):43-44,54.
[2]	Guliyev VB,Gul M,Yildirim A.Hippophae rhamnoides L.:chromatographic methods to determine chemical composition,use in traditional medicine and pharmacological effects[J].J Chromatogr B,2004,812(1/2):291-307.
[3]	Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典)[S].Beijing:China Medical Science Press,2015:184-185.
[4]	Liu Y,Lian YS,Wang YL,et al.Review of research and development and significant effect of Hippophae rhamnoides[J].Chin J Chin Materia Medica(中国中药杂志),2014,39(9):1547-1552.
[5]	Yogendra Kumar MS,Tirpude RJ,Maheshwari DT,et al.Antioxidant and antimicrobial properties of phenolic rich fraction of seabuckthorn(Hippophae rhamnoides L.)leaves in vitro[J].Food Chem,2013,141(4):3443-3450.
[6]	Pallavi B, Chandresh V, Kanika K, et al. In vitro evaluation of antidiabetic and antioxidant activity of seabuckthorn(Hippophae rhamnoides L.)leaves[J].J Med Plant Res,2015,9(35):929-932.
[7]	Bao M,Lou Y.Flavonoids from seabuckthorn protect endothelial cells(EA.hy926)from oxidized low-density lipoprotein induced injuries via regulation of LOX-1 and eNOS expression[J].J Cardiovasc Pharmacol,2006,48(1):834-841.
[8]	Cheng J,Kondo K,Suzuki Y,et al.Inhibitory effects of total flavones of Hippophae rhamnoides L.on thrombosis in mouse femoral artery and in vitro platelet aggregation.[J].Life Sci,2003,72(20):2263-2271.
[9]	Ganju L,Padwad Y,Singh R,et al.Anti- inflammatory activity of seabuckthorn(Hippophae rhamnoides)leaves[J].Int J Immunopharmacol,2005,5(12):1675-1684.
[10]	Geetha S,Ram MS,Sharma SK,et al.Cytoprotective and antioxidant activity of seabuckthorn(Hippophae rhamnoides L.)flavones against tert-butyl hydroperoxide-induced cytotoxicity in lymphocytes.[J].J Med Food,2009,12(1):151-158.
[11]	Zhou JY,Zhou SW,Du XH,et al.Protective effect of total flavonoids of seabuckthorn(Hippophaerhamnoides)in simulated high-altitude polycythemia in rats[J].Molecules,2012,17(10):11585-11597.
[12]	Rösch D,Krumbein A,Mügge,et al.Structural investigations of flavonol glycosides from sea buckthorn(Hippophaë rhamnoides)pomace by NMR spectroscopy and HPLC-ESI-MSn[J].J Agric Food Chem,2004,52(13):4039-4046.
[13]	Wei G,Hou X.HPLC method in determining the content of quercetin,kaempferide and isorhamnetin in seabuckthorn flavone powder[J].Global Seabuckthorn Res Dev(国际沙棘研究与开发),2014,12(2):22-24.
[14]	Hui R,Feng J,Ling M,et al.Multicriteria comprehensive assessment on the optimization of extraction technology of Hippophae rhamnoides Linn.flavonoids from leaves and seeds[J].China Pharm(中国药房),accepted.
[15]	Du L,Zhang H,Xiao W,et al.Comparative determination of total flavonoids in seabuckthron leaves from 10 species by RP-HPLC[C].The Fifth Shanghai International Conference on Traditional Chinese Medicine and Natural Medicine.2012:281-285.

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Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD

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