• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
LIANG Fangmei, NI Yueling, WANG Lu, HANG Taijun, SONG Min. Structural identification of the related substances of fusidic acid by LC-MS[J]. Journal of China Pharmaceutical University, 2018, 49(3): 322-332. DOI: 10.11665/j.issn.1000-5048.20180311
Citation: LIANG Fangmei, NI Yueling, WANG Lu, HANG Taijun, SONG Min. Structural identification of the related substances of fusidic acid by LC-MS[J]. Journal of China Pharmaceutical University, 2018, 49(3): 322-332. DOI: 10.11665/j.issn.1000-5048.20180311
shu

Structural identification of the related substances of fusidic acid by LC-MS

More Information
    Graphical Abstract
    • Abstract
  • To identify the related substances of fusidic acid by LC-MS, separation was performed on an Agilent Extend-C18 column(150 mm × 4. 6 mm, 3. 5 μm)by linear gradient elution with a mobile phase consisting of methanol, acetonitrile and formic acid. Electrospray positive ionization high resolution TOF/MS was used for the determination of the accurate mass and elemental composition of parent ions of the related substances; triple quadrupole tandem mass was employed for the mass spectra determination of the product. The structures of the related substances were then figured out through the elucidation of the fragment ions. Fusidic acid and its related substances were adequately separated under the established HPLC conditions. Nineteen major related substances of fusidic acid were detected and speculated by hyphenated techniques. Eleven of them were recorded in European Pharmacopoeia, while the others have not been previously reported. The established LC-MS method is effective for the separation and identification of the related substances of fusidic acid and the results are useful for its storage conditions and quality assurance.
    • FullText(HTML)
    • References (12)
  • [1]
    Frosini SM,Bond R,Loeffler A,et al.Opportunities for topical antimicrobial therapy:permeation of canine skin by fusidic acid[J].Bmc Vet Res,2017,13(1):345-346.
    [2]
    Alsterholm M, Flytstrom I, Bergbrant IM, et al. Fusidic acid-resistant Staphylococcus aureus in impetigo contagiosa and secondarily infected atopic dermatitis[J].Acta Derm Venereol,2010,90(1):52-57.
    [3]
    Doudoulakakis A,Spiliopoulou I,Spyridis N,et al.Emergence of a Staphylococcus aureus clone resistant to mupirocin and fusidic acid carrying exotoxin genes and causing mainly skin infections[J].J Clin Microbiol,2017,55(8):2529-2537.
    [4]
    Belardinelli R,Rodnina MV.Effect of fusidic acid on the kinetics of molecular motions during EF-G-induced translocation on the ribosome[J].Sci Rep,2017,7(1):10536.
    [5]
    Li B,Li B,Zhou C.Progress on antimicrobial peptides against drug-resistant bacterial infection[J].J China Pharm Univ(中国药科大学学报),2014,45(5):580-586.
    [6]
    Curbete MM,Salgado HR.A critical review of the properties of fusidic acid and analytical methods for its determination[J].Crit Rev Anal Chem,2016,46(4):352-360.
    [7]
    Goswami N,Gupta VR,Jogia HA.Development and validation of a novel stability-indicating RP-HPLC method for the simultaneous determination of halometasone,fusidic acid,methylparaben,and propylparaben in topical pharmaceutical formulation[J].Sci Pharm,2013,81(2):505-518.
    [8]
    Morgan J,Joyce-Menekse ME,Rowlands RT,et al.Rapid and sensitive quantitation of antibiotics in fermentations by electrospray mass spectrometry[J].Rapid Commun Mass Spectrom,2001,15(14):1229-1238.
    [9]
    Nawaz M,Arayne MS,Sultana N,et al.Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC[J].Acta Chromatogr,2014,26(1):57-66.
    [10]
    Curbete MM,Salgado HRN.Stability-indicating RP-LC method for quantification of fusidic acid in cream[J].Braz J Pharm Sci,2016,52(3):447-457.
    [11]
    Curbete MM,Salgado HRN.Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream[J].Talanta,2016,153:51-56.
    [12]
    Yin XY,Wang C,Ming GJ,et al.Identification of the related substances of pioglitazone hydrochloride by hyphenated LC-MS techniques[J].J China Pharm Univ(中国药科大学学报),2017,48(6):701-710.
    • Related Articles

  • Related Articles

    [1]WANG Shihao, LIU Lifeng, DING Yang, LI Suxin. Research progress of pH-responsive drug delivery systems in cancer immunotherapy[J]. Journal of China Pharmaceutical University, 2024, 55(4): 522-529. DOI: 10.11665/j.issn.1000-5048.2024011902
    [2]BAI Yan, GUO Xinyao, QIN Qiliang, YAN Fang. Advances in research on mechanism of lactate dehydrogenase B in tumors[J]. Journal of China Pharmaceutical University, 2023, 54(2): 172-179. DOI: 10.11665/j.issn.1000-5048.20221112001
    [3]XING Xuyang, WANG Xiaochun, HE Wei. Advances in research on tumor immunotherapy and its drug development[J]. Journal of China Pharmaceutical University, 2021, 52(1): 10-19. DOI: 10.11665/j.issn.1000-5048.20210102
    [4]LI Fang, XIN Junbo, SHI Qin, MAO Chengqiong. Advances in near infrared photoimmunotherapy of tumor[J]. Journal of China Pharmaceutical University, 2020, 51(6): 664-674. DOI: 10.11665/j.issn.1000-5048.20200604
    [5]FENG Yang, XU Xiao, MO Ran. Advances in lymphatic targeted drug delivery system for treatment of tumor metastasis[J]. Journal of China Pharmaceutical University, 2020, 51(4): 425-432. DOI: 10.11665/j.issn.1000-5048.20200406
    [6]TANG Keqin, LIN Huaqing, LI Shuhong, DONG Lixin, LU Bohong, JIANG Hong. Advances in tumor targeted nanocrystals[J]. Journal of China Pharmaceutical University, 2020, 51(4): 418-424. DOI: 10.11665/j.issn.1000-5048.20200405
    [7]LI Haoxian, LIN Huaqing, CHEN Jingwen, WANG Liyuan. Research progress of carbon nanomaterials in cancer drug delivery[J]. Journal of China Pharmaceutical University, 2019, 50(1): 100-106. DOI: 10.11665/j.issn.1000-5048.20190114
    [8]DONG Hong, WU Ruixue, LIU Jiaqi, HUANG Qing, ZHOU Ya, HU Yiqiao. Advances in cancer photodynamic therapy[J]. Journal of China Pharmaceutical University, 2016, 47(4): 377-387. DOI: 10.11665/j.issn.1000-5048.20160401
    [9]SUN Zhan-yi, CAI Hui, HUANG Zhi-hua, SHI Lei, CHEN Yong-xiang, LI Yan-mei. Advances of glycopeptide-associated tumor vaccines[J]. Journal of China Pharmaceutical University, 2012, 43(2): 97-106.
    [10]YANG Li, YOU Qi-dong, YANG Yong, GUO Qing-long. Research advances in wogonin′s anti-tumor effects[J]. Journal of China Pharmaceutical University, 2009, 40(6): 576-579.

Catalog

    Get Citation
    PDF
    XML
    Article views (853) PDF downloads (1293) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：365909

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return