• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
LI Xue, AI Yongling, HE Qunye, YANG Gongjun, WANG Jing. Detection of prostate specific antigen-based on dual signal amplified electrochemical immunoassay[J]. Journal of China Pharmaceutical University, 2018, 49(4): 433-440. DOI: 10.11665/j.issn.1000-5048.20180408
Citation: LI Xue, AI Yongling, HE Qunye, YANG Gongjun, WANG Jing. Detection of prostate specific antigen-based on dual signal amplified electrochemical immunoassay[J]. Journal of China Pharmaceutical University, 2018, 49(4): 433-440. DOI: 10.11665/j.issn.1000-5048.20180408

Detection of prostate specific antigen-based on dual signal amplified electrochemical immunoassay

More Information
  • A sandwiched electrochemical immunoassay based on the AuNPs@GSH-CdTe as a signal label, which formed by GSH-CdTe QDs and AuNPs, with dual signal amplified by reduced graphene oxide and AuNPs was proposed for the sensitive detection of prostate specific antigen(PSA). Through a sandwich immunoreaction, the target PSA and AuNPs@GSH-CdTe labeled Ab1 were captured to rGO/AuNPs-Ab2 surface. After the HNO3-dissolution step, square wave stripping voltammetry(SWSV)analysis of the captured CdTe QDs was used to quantify the concentration of PSA. In this system, AuNPs possessedlarge specific surface and good biocompatibility, which could effectively expand the amount of antigen and GSH-CdTe QDs loading and signals amplifying, while rGO played a synergistic amplification role due to its large specific surface. The proposed method showed good linearity ranging from 0. 5 to 200 ng/mL with the detection limits of 5. 0 pg/mL. It also showed excellent selectivity, good reproducibility, satisfactory stability. In addition, the method was successfully applied to the determination of real samples. The result was satisfactory and the recovery could fall in 98. 20%- 106. 2%, which represented a novel approach for versatile detection of tumor markers.
  • [1]
    Jie GF,Huang HP,Sun XL,et al.Electrochemiluminescence of CdSe quantum dots for immunosensing of human prealbumin[J].Biosens Bioelectron,2008,23(12):1896-1899.
    [2]
    Ertürk G, Özen H, Tümer MA, et al. Microcontact imprinting based surface plasmon resonance(SPR)biosensor for real-time and ultrasensitive detection of prostate specific antigen(PSA)from clinical samples[J].Sensor Actuat B-Chem,2016,224:823-832.
    [3]
    Wei Q,Zhao YF,Xu CX,et al.Nanoporous gold film based immunosensor for label-free detection of cancer biomarker[J].Biosens Bioelectron,2011,26(8):3714-3718.
    [4]
    Wang R,Wang AJ,Liu WD,et al.A novel label-free electrochemical immunosensor for ultra-sensitively detecting prostate specific antigen based on the enhanced catalytic currents of oxygen reduction catalyzed by core-shell Au@Pt nanocrystals[J].Biosens Bioelectron,2018,102:276-281.
    [5]
    Xue B,Deng DW,Cao J,et al.Synthesis of violet-near-infrared-emitting quantum dots using N-acetyl-L-cysteine as ligand[J].J China Pharm Univ(中国药科大学学报),2010,41(5):456-461.
    [6]
    Wang J,Shan Y,Xu JJ,et al.Gold nanoparticle enhanced electrochemiluminescence of CdS thin films for ultrasensitive thrombin detection[J].Anal Chem,2011,83(11):4004-4011.
    [7]
    Lu HJ, Zhao W, Xu JJ, et al. Visual electrochemiluminescence ratiometry on bipolar electrode for bioanalysis[J].Biosens Bioelectron,2018,102:624-630.
    [8]
    Song FJ,Ai YL,Zhong WY,et al.Detection of copper ions and glutathione based on off-on fluorescent graphene quantum dots[J].J China Pharm Univ(中国药科大学学报),2018,49(1):87-92.
    [9]
    Cui RJ,Pan HC,Zhu JJ,et al.Versatile immunosensor using CdTe quantum dots as electrochemical and fluorescent labels[J].Anal Chem,2007,79:8494-8501.
    [10]
    Liu Y,Zhu L,Kong JL,et al.A quantum dots-based electrochemical assay towards the sensitive detection of tumor cells[J].Electrochem Commun,2013,33:59-62.
    [11]
    Tong P,Zhao WW,Zhang L,et al.Double-probe signal enhancing strategy for toxin aptasensing based on rolling circle amplification[J].Biosens Bioelectron,2012,33(1):146-151.
    [12]
    Kumar S, Ashish, Kumar S, et al. Effect of Brownian motion on reduced agglomeration of nanostructured metal oxide towards development of efficient cancer biosensor[J].Biosens Bioelectron,2018,102:247-255.
    [13]
    Li JJ,Yan XX,Li XY,et al.A new electrochemical immunosensor for sensitive detection of prion based on Prussian blue analogue[J].Talanta,2018,179:726-733.
    [14]
    Pang YH,Zhang Y,Li QY,et al.Synergetic accumulation and simultaneous determination of naphthol isomers on electrochemically reduced graphene oxide modified electrode[J].J Electroanal Chem,2016,769:89-96.
    [15]
    Ma JC,Zhang WD.Gold nanoparticle-coated multiwall carbon nanotube-modified electrode for electrochemical determination of methyl parathion[J].Microchim Acta,2011,175(3/4):309-314.
    [16]
    Zhao WW,Dong XY,Wang J,et al.Immunogold labeling-induced synergy effect for amplified photoelectrochemical immunoassay of prostate-specific antigen[J].Chem Commun,2012,48(43):5253-5255.
    [17]
    Zhang LJ,Xu CL,Li BX.Simple and sensitive detection method for chromium(VI)in water using glutathione-capped CdTe quantum dots as fluorescent probes[J].Microchim Acta,2009,166(1/2):61-68.
    [18]
    Rabbani MM,Nam DG,Kim DH,et al.Characterization of Au/CdTe nanocomposites prepared by electrostatic interaction[J].T Nonferr Metal Soc,2013,23(2):426-432.
    [19]
    Tuz Johra F,Jung WG.Low temperature synthesis of RGO-Au nanocomposite with apparently reduced time and its application as a chemical sensor[J].Appl Surf Sci,2016,362:169-175.
    [20]
    Yu WW,Qu LH,Guo WZ,et al.Experimental determination of the extinction coefficient of CdTe,CdSe,and CdS Nanocrystals[J].Chem Mater,2003,15:2854-2860.
    [21]
    Liu RP,Wang C,Jiang Q,et al.Magnetic-particle-based,ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen[J].Anal Chim Acta,2013,801:91-96.
    [22]
    Xu SJ, Liu Y, Wang TH, et al. Positive potential operation of a cathodic electrogenerated chemiluminescence immunosensor based on luminol and graphene for cancer biomarker detection[J].Anal Chem,2011,83:3817-3823.
    [23]
    Gao ZQ,Xu MD,Tang DP,et al.Magnetic bead-based reverse colorimetric immunoassay strategy for sensing biomolecules[J].Anal Chem,2013,85:6945-6954.
    [24]
    Lakkavarapu S,Pradeep Kumer B,Bondili JS,et al.Development of an electrochemical immunosensor based on gold nanoparticles incorporated chitosan biopolymer nanocomposite film for the detection of prostate cancer using PSA as biomarker[J].Enzyme Microb Tech,2018,112:43-51.
    [25]
    Xu J,Zhang QM,Xie HY,et al.High sensitive detection method for protein by combining the magnetic separation with cation exchange based signal amplification[J].Talanta,2017,168:91-99.
  • Related Articles

    [1]WANG Songkai, ZOU Yuchen, SUN Shipeng, YAN Zhiye, TANG Weiwei, LI Ping, LI Bin. Recent advances in mass spectrometry imaging and its application in drug research[J]. Journal of China Pharmaceutical University, 2023, 54(6): 653-661. DOI: 10.11665/j.issn.1000-5048.2023091901
    [2]FENG Yang, XU Xiao, MO Ran. Advances in lymphatic targeted drug delivery system for treatment of tumor metastasis[J]. Journal of China Pharmaceutical University, 2020, 51(4): 425-432. DOI: 10.11665/j.issn.1000-5048.20200406
    [3]ZHOU Yeshu, WANG Yanmei, ZHANG Beiyuan, WU Shuaicong, YANG Lei, YIN Lifang. Research progress of inorganic nanomaterials in drug delivery system[J]. Journal of China Pharmaceutical University, 2020, 51(4): 394-405. DOI: 10.11665/j.issn.1000-5048.20200403
    [4]WEI Yuanyuan, YANG Fan, TANG Jie, YU Lifang. Advances in the research of anti-tuberculosis drugs[J]. Journal of China Pharmaceutical University, 2020, 51(2): 231-239. DOI: 10.11665/j.issn.1000-5048.20200215
    [5]HAN Xiu, QI Xiaole, WU Zhenghong. Advances in self-assemblied DNA nanocages as drug delivery systems[J]. Journal of China Pharmaceutical University, 2017, 48(6): 663-669. DOI: 10.11665/j.issn.1000-5048.20170605
    [6]CHEN Xing, KANG Yang, WU Jun. Advances in biodegradable functional polymers based protein drug delivery system[J]. Journal of China Pharmaceutical University, 2017, 48(2): 142-149. DOI: 10.11665/j.issn.1000-5048.20170203
    [7]JIANG Lu, CHEN Dandan, SUN Minjie, PING Qineng, ZHANG Can. Advances of wax matrix tablets[J]. Journal of China Pharmaceutical University, 2016, 47(4): 497-502. DOI: 10.11665/j.issn.1000-5048.20160418
    [8]YAO Guilin, WANG Haiyong, LU Tao. Advances of the uricosuric drugs[J]. Journal of China Pharmaceutical University, 2016, 47(4): 491-496. DOI: 10.11665/j.issn.1000-5048.20160417
    [9]LIU Yanhong, ZHOU Jianping, HUO Meirong. Advances in the tumor microenvironment-responsive smart drug delivery nanosystem[J]. Journal of China Pharmaceutical University, 2016, 47(2): 125-133. DOI: 10.11665/j.issn.1000-5048.20160201
    [10]WANG Ruoning, LIU Congyan, ZHOU Jianping, CHEN Jian, WANG Wei. Advances in the research of lipoprotein-based nano scale drug delivery systems[J]. Journal of China Pharmaceutical University, 2014, 45(1): 10-16. DOI: 10.11665/j.issn.1000-5048.20140102

Catalog

    Article views (1143) PDF downloads (1350) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return