• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
CAO Guoxiu, LU Wenjie, YE Hui, TIAN Yang, HAO Haiping. Rapid identification of constituents from different Ginkgo biloba preparations by high resolution mass spectrometry and metabolomics technology[J]. Journal of China Pharmaceutical University, 2018, 49(4): 441-448. DOI: 10.11665/j.issn.1000-5048.20180409
Citation: CAO Guoxiu, LU Wenjie, YE Hui, TIAN Yang, HAO Haiping. Rapid identification of constituents from different Ginkgo biloba preparations by high resolution mass spectrometry and metabolomics technology[J]. Journal of China Pharmaceutical University, 2018, 49(4): 441-448. DOI: 10.11665/j.issn.1000-5048.20180409

Rapid identification of constituents from different Ginkgo biloba preparations by high resolution mass spectrometry and metabolomics technology

More Information
  • A qualitative analysis was developed to identify different ingredients from three Ginkgo biloba preparations(including Yinxingye Diwan, extract of Ginkgo biloba leaves tablets and Yinxingtongzhi Diwan)based on high resolution mass spectrometry and metabolomics technology. An XSELECT HSS T3(4. 6 mm×150 mm, 3. 5 μm)column was used for separation, with the mobile phases consisting of acetonitrile and water containing 0. 1% formic acid. The column temperature was set at 40 °C. Negative ion mode was used for mass spectrometric data acquisition. Through partial least squares projection to latent structure-discriminant analysis(PLS-DA), we firstly found the different compounds among different Ginkgo biloba preparations. Subsequently, the compounds displaying different abundance levels via database searching and literature matching were identified. Finally, we identified 21 different compounds between the extract of Ginkgo biloba leaves tablets and Yinxingye Diwan, which mostly belong to the organic acids and flavonols families. Quantitative analysis showed that the ingredients which had higher abundance in extract of Ginkgo biloba leaves tablets were mainly organic acids, whereas those exhibiting higher levels in Yinxingye Diwan are flavonols. We also identified 12 different ingredients between the Yinxingye Diwan and Yinxingtongzhi Diwan, which were flavonols, sciadopitysin and organic acids. The results of this study are useful for studying different chemical constituents from distinct Ginkgo biloba preparations.
  • [1]
    Liu XG,Cheng CY,Wang JX,et al. A metabolic exposure-oriented network regulation strategy for the identification of effective combination in the extract of Ginkgo biloba L.[J].J Pharm Biomed Anal,2018,149:151-159.
    [2]
    Avula B,Sagi S,Gafner S,et al. Identification of Ginkgo biloba supplements adulteration using high performance thin layer chromatography and ultra high performance liquid chromatography-diode array detector-quadrupole time of flight-mass spectrometry[J].Anal Bioanal Chem,2015,407(25):7733-7746.
    [3]
    DeFeudis FV,Drieu K.Ginkgo biloba extract(EGb 761)and CNS functions:basic studies and clinical applications[J].Curr Drug Targets,2000,1(1):25-58.
    [4]
    Liebgott T,Miollan M,Berchadsky Y,et al. Complementary cardioprotective effects of flavonoid metabolites and terpenoid constituents of Ginkgo biloba extract(EGb 761)during ischemia and reperfusion[J].Basic Res Cardiol,2000,95(5):368-377.
    [5]
    Weng XJ,Chen LL,Zhang HQ.Effect of total flavonoid in leaves of Ginkgo biloba on the apoptosis of eosinophil in broncho alveloar lavage flui[J].Acta Pharm Sin(药学学报),2008,43(5):480-483.
    [6]
    Geng XH,Yang QC,Lu P,et al.Therapeutic time window of dimethylaminoethyl ginkgolide B mesylate in focal cerebral ischemia of rats[J].J China Pharm Univ(中国药科大学学报),2011,42(2):149-152.
    [7]
    Li WZ,Wu WY,Huang H,et al. Protective effect of bilobalide on learning and memory impairment in rats with vascular dementia[J].Mol Med Rep,2013,8(3):935-941.
    [8]
    Yuan Y,Zhang PY,Jin SY,et al.Effects of ginkgolide on early apoptosis of rat cortical neurons induced by hypoxia[J].J China Pharm Univ(中国药科大学学报),2003,34(5):465-468.
    [9]
    He J,Yang S,Yang J,et al. Extraction of ginkgolides from Ginkgo biloba[J].Chin J Chin Mater Med(中国中药杂志),2010,35(9):1127-1129.
    [10]
    Lichtblau D,Berger JM,Nakanishi K.Efficient extraction of ginkgolides and bilobalide from Ginkgo biloba leaves[J].J Nat Prod,2002,65(10):1501-1504.
    [11]
    Lopez-Gutierrez N,Romero-Gonzalez R,Vidal JLM,et al. Quality control evaluation of nutraceutical products from Ginkgo biloba using liquid chromatography coupled to high resolution mass spectrometry[J].J Pharm Biomed Anal,2016,121:151-160.
    [12]
    Liu XG,Wu SQ,Li P,et al. Advancement in the chemical analysis and quality control of flavonoid in Ginkgo biloba[J].J Pharm Biomed Anal,2015,113:212-225.
    [13]
    van Beek TA,Montoro P.Chemical analysis and quality control of Ginkgo biloba leaves,extracts,and phytopharmaceuticals[J].J Chromatogr A,2009,1216(11):2002-2032.
    [14]
    Wagner S,Scholz K,Sieber M,et al. Tools in metabonomics:an integrated validation approach for LC-MS metabolic profiling of mercapturic acids in human urine[J].Anal Chem,2007,79(7):2918-2926.

Catalog

    Article views (1347) PDF downloads (1489) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return