Chemical constituents from the leaves of Anisopus mannii and the melanogenesis inhibitory activities

The phytochemical studies on the leaves of Anisopus mannii led to the isolation of seven compounds by silica gel, ODS, DIAION HP-20, Sephadex LH-20 colunmn chromatographer, their structures were elucidated on the basis of the spectroscopic analyses(NMR, HRMS)and the comparisons with the literatures as 3β-acetoxylup-20(29)-ene( 1 ), 1-acetoxy-2-isopropyl-1-tridecene( 2 ), rutin( 3 ), 3, 6′-diferuloylsucrose( 4 ), 3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid 28-O-［α-L-rhamnopyra- nosyl-(1→2)-β-D-glucopyranosyl］ ester( 5 ), conduritol A( 6 ), hoyacarnoside I( 7 ). Meanwhile, the isolated compounds were evaluated for their inhibitory activities against melanogensis in B16 melanoma cells, as the results, all compounds exhibited melanogenesis-inhibitory activity and compound 5 showed a strongest activity(Melanin content: (27. 4±3. 5)%, Cell Viability: (54. 9±5. 6)% with a concentration of 30 μmol/L)which could be further developed.