• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHOU Min, GAO Huixin, FENG Yi, ZENG Zhaoqing, TIAN Hui. Analysis on genetic diversity of Hedyotis diffusa Willd.by inter simple sequence repeat(ISSR)[J]. Journal of China Pharmaceutical University, 2019, 50(2): 200-205. DOI: 10.11665/j.issn.1000-5048.20190211
Citation: ZHOU Min, GAO Huixin, FENG Yi, ZENG Zhaoqing, TIAN Hui. Analysis on genetic diversity of Hedyotis diffusa Willd.by inter simple sequence repeat(ISSR)[J]. Journal of China Pharmaceutical University, 2019, 50(2): 200-205. DOI: 10.11665/j.issn.1000-5048.20190211

Analysis on genetic diversity of Hedyotis diffusa Willd.by inter simple sequence repeat(ISSR)

More Information
  • This research used inter simple sequence repeat(ISSR)markers to analyze the genetic diversity of Hedyotis diffusa Willd. from different origins. A total of 23 samples of Hedyotis diffusa Willd. in the Guangxi Zhuang Autonomous Region, Guangdong, Hunan, Zhejiang, Fujian, Jiangxi and Anhui, respectively were collected. 150 ISSR primers were used to amplify PCR and then POPGENE1. 32, NTSYS2. 10 software were used to analyze genetic diversity. 11 primers were screened, 115 polymorphic bands were amplified, the polymorphism ratio was 85. 22%, the number of alleles(Na)was 1. 852 2, the effective allele(Ne)was 1. 543 4, Neis gene diversity index(H)was 0. 316 5 and Shannon′s information index(I)was 0. 470 0. The results of cluster analysis show that the Hedyotis diffusa can be divided into three clades. The conclusion is that ISSR molecular markers can provide a insight for the identification of Hedyotis diffusa Willd. .
  • [1]
    Guangxi Food And Drug Administration. Quality Standard of Zhuang Medicine in Guangxi Zhuang Autonomous Region[M].Nanning:Guangxi Science & Technology Publishing House Co.,Ltd.,2008:82-84.
    [2]
    Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:Part 1(中华人民共和国药典:一部)[S].Beijing China Medical Science Press,2015:23.
    [3]
    Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genonics,1994, 20(2):176-183.
    [4]
    He G,Zhu LF,Liu XG,et al.Analysis on genetic diversity of thirteen Curculigo Gaertn.populations by ISSR markers[J].Nonwood Forest Res(经济林研究),2016,34(3):84-89.
    [5]
    Jiang JL,Ye W,Li YQ,et al.Genetic relationships among wild Dendrobium officinale populations in Taining,Fujian by ISSR[J].J Tropical Subtropical Botany(热带亚热带植物学报),2016,24(3):259-266.
    [6]
    Jiang YH, Zhang LP, Zhou XG, et al. Application research on relationship of Paeonialactiflora Pall.by ISSR[J].World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术-中医药现代化),2016,18(3):429-433.
    [7]
    Guo L. Genetic diversity of Magnolia Biondii(Magnoliaceae)assessed with ISSR polymorphisms(望春玉兰种质资源遗传多样性ISSR分析)[D].Northwest University,2014.
    [8]
    Liu YJ,Huang Y,Rong JD,et al.Genetic diversity analysis of Morinda offlcinalis by ISSR markers[J].J Fujian Coll Forestry(福建林学院学报),2011,31(3):203-206.
    [9]
    Editorial Committee of Botany of the Chinese Academy of Sciences.Flora of China(中国植物志)[M].Beijing:Scientific and Technical Publishers,1979:75.
    [10]
    Zhang SY,Wang TJ,Li WQ,et al.Genetic differentiation of the different geographical populations of Artemisia sphaerocephala by inter-simple sequence repeat(ISSR)[J].Bull Botanical Res(植物研究),2010,30(4):455-460.
  • Related Articles

    [1]WANG Xuefang, ZHAO Yang, LIU Zhuqing, GUO Le, ZHONG Feiliang, LUO Xuegang. Genetic engineering and molecular modification of recombinant fully humanized single-domain antibody against Helicobacter pylori UreB[J]. Journal of China Pharmaceutical University, 2024, 55(5): 666-672. DOI: 10.11665/j.issn.1000-5048.2023122903
    [2]KONG Ying, YE Hui, SHAO Chang, HAO Haiping. Applications of genetic code expansion in the study of lysine acylation[J]. Journal of China Pharmaceutical University, 2023, 54(5): 519-526. DOI: 10.11665/j.issn.1000-5048.2023041401
    [3]XU Qianzi, LIANG Yueru, ZHAO Zhongwei, SHEN Yan. Population bioequivalence statistical method based on earth mover′s distance for evaluation of particle size distribution consistency of ophthalmic emulsion[J]. Journal of China Pharmaceutical University, 2022, 53(6): 698-704. DOI: 10.11665/j.issn.1000-5048.20220608
    [4]ZHU Yinxue, WANG Dexiang, KONG Ying, LU Wenjie, YE Hui, HAO Haiping. Genetic incorporation of unnatural amino acids into proteins and its translational application in biomedicine[J]. Journal of China Pharmaceutical University, 2022, 53(4): 383-391. DOI: 10.11665/j.issn.1000-5048.20220401
    [5]LIU Hengping, WANG Jinzheng, GAO Chengzhe, XU Bin, LI Qingran, LIN Xinhao, LIN Kejiang. Key fingerprint fragments of anti-hepatitis B virus agents using genetic function approximation method[J]. Journal of China Pharmaceutical University, 2014, 45(4): 405-409. DOI: 10.11665/j.issn.1000-5048.20140404
    [6]YAO Dongning, XU Xiaowei, YU Huanbing, LI You, LI Xing, GAO Xiangdong, TIAN Hong. Screening system for amber suppressor tRNA based on inducible high-copy-number plasmid[J]. Journal of China Pharmaceutical University, 2013, 44(2): 174-178. DOI: 10.11665/j.issn.1000-5048.20130214
    [7]ZHANG Ji-xing, CHEN Yan-zhong. Optimization of the process parameters of Baeckeae oil-β-cyclodextrin inclusion complex by artificial neural network and genetic algorithm[J]. Journal of China Pharmaceutical University, 2011, 42(4): 324-328.
    [8]Microscopic ldentification Studies on Dendrobium Stems By Clustering AnalysisMa[J]. Journal of China Pharmaceutical University, 1996, (4): 19-21.
    [9]CHROMOSOME VARIATIONS IN THE CELL CULTURE OF PANAX GINSENG[J]. Journal of China Pharmaceutical University, 1988, (2): 84-89.
    [10]PROTOPLAST FUSION AND GENETIC TRANSFER OF STAPHYLOCOCCI[J]. Journal of China Pharmaceutical University, 1987, (1): 31-35.

Catalog

    Article views (592) PDF downloads (876) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return