Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases

ZHENG Binbin; ZHANG Jianan; FAN Yixin; HU Liang; LIU Wentao; WANG Xuerong

doi:10.11665/j.issn.1000-5048.20200208

Journal of China Pharmaceutical University > 2020 > 51(2): 180-184. > DOI: 10.11665/j.issn.1000-5048.20200208

ZHENG Binbin, ZHANG Jianan, FAN Yixin, HU Liang, LIU Wentao, WANG Xuerong. Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases[J]. Journal of China Pharmaceutical University, 2020, 51(2): 180-184. DOI: 10.11665/j.issn.1000-5048.20200208

Citation:

PDF (1109 KB)

Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases

More Information

Graphical Abstract

Abstract

Abstract

The aim of the present study was to investigate the effects and mechanisms of lidocaine on lipopolysaccharide(LPS)-induced matrix metalloproteinase(MMP)-9 and MMP-2 activity in plasma, and the effects of lidocaine on LPS-induced acute lung injury(ALI). Mice were pretreated with lidocaine(2, 4, 8 mg/kg )for 30 minutes, and then treated with 10 mg/kg LPS(ip)for 12 h to induce ALI. The 7-day survival rate and lung wet/dry weight ratio of mice were monitored. Phosphorylation level of p38 was measured by western blot. The activity of MMP-9 and MMP-2 in plasma was evaluated by gelatin zymography. The results showed that pretreatment with lidocaine could significantly reduce the death rate of ALI mice as well as the lung wet/dry weight ratio in a dose-dependent manner and suppress the activity of MMP-9 and MMP-2 in plasma. Moreover, lidocaine also markedly inhibited LPS-induced upregulation of p-p38 in a dose-dependent manner. In conclusion, lidocaine alleviated LPS-induced acute lung injury by suppressing MMP-9 and MMP-2 activity.
- lidocaine,
- lipopolysaccharide,
- acute lung injury,
- matrix metalloproteinases

FullText(HTML)

References (19)

References

[1]	Hung YL, Fang SH, Wang SC, et al. Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response[J].Sci Rep,2017,7:46299.
[2]	Hirano Y,Aziz M,Yang WL,et al.Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury[J].Crit Care,2015,19(1):53.
[3]	Matthay MA,Ware LB,Zimmerman GA.The acute respiratory distress syndrome[J].J Clin Invest,2012,122(8):2731-2740.
[4]	Rhee C,Dantes R,Epstein L,et al.Incidence and trends of sepsis in US hospitals using clinical vs claims data,2009-2014[J].Jama,2017,318(13):1241-1249.
[5]	Keck T,Balcom JH,Fernández-del Castillo C,et al.Matrix metalloproteinase-9 promotes neutrophil migration and alveolar capillary leakage in pancreatitis-associated lung injury in the rat[J].Gastroenterology,2002,122(1):188-201.
[6]	Sen AI, Foronjy RF, Shiomi T, et al. Loss of MMP-13 delays inflammatory response after acute hyperoxic lung injury[J].Proc Am Thorac Soc,2008,5(3):360-361.
[7]	Zhang F, Hu L, Wu YX, et al. Doxycycline alleviates paraquat-induced acute lung injury by inhibiting neutrophil-derived matrix metalloproteinase 9[J].Int Immunopharmacol,2019,72: 243-251.
[8]	Zinter MS,Delucchi KL,Kong MY,et al.Early plasma matrix metalloproteinase profiles.A novel pathway in pediatric acute respiratory distress syndrome[J].Am J Respir Crit Care Med,2019,199(2):181-189.
[9]	Chakrabarti S,Patel KD.Matrix metalloproteinase-2(MMP-2)and MMP-9 in pulmonary pathology[J].Exp Lung Res,2005,31(6):599-621.
[10]	Fan YX,Liu HJ,Li YX,et al.Quercetin ameliorates postoperative pain by suppressing matrix metalloproteinase in microglia[J].J China Pharm Univ(中国药科大学学报),2017,48(3):343-347.
[11]	Xu XL,Ji H,Gu SY,et al.Effects of astragaloside IV on experimental ventricular remodeling in mice and its mechanism from matrix metalloproteinase aspect[J].J China Pharm Univ(中国药科大学学报),2010,41(1):70-75.
[12]	Ricou B,Nicod L,Lacraz S,et al.Matrix metalloproteinases and TIMP in acute respiratory distress syndrome[J].Am J Respir Crit Care Med,1996,154(2 Pt 1):346-352.
[13]	Fujita M,Harada E,Ikegame S,et al.Doxycycline attenuated lung injury by its biological effect apart from its antimicrobial function[J].Pulm Pharmacol Ther,2007,20(6):669-675.
[14]	Carney DE, Mccann UG, Schiller HJ, et al. Metalloproteinase inhibition prevents acute respiratory distress syndrome[J].J Surg Res,2001,99(2):245-252.
[15]	Zhang Z,Zhou J,Liao C,et al.RAGE deficiency attenuates the protective effect of Lidocaine against sepsis-induced acute lung injury[J].Inflammation,2017,40(2):601-611.
[16]	Zhang H,Sha J,Feng X,et al.Dexmedetomidine ameliorates LPS induced acute lung injury via GSK-3β/STAT3-NF-κB signaling pathway in rats[J].Int Immunopharmacol,2019,74:105717.
[17]	Vassallo A,Wood AJ,Subburayalu J,et al.The counter-intuitive role of the neutrophil in the acute respiratory distress syndrome[J].Br Med Bull,2019,131(1):43-55.
[18]	Chen LJ,Ding YB,Ma PL,et al.The protective effect of lidocaine on lipopolysaccharide-induced acute lung injury in rats through NF-κB and p38 MAPK signaling pathway and excessive inflammatory responses[J].Eur Rev Med Pharmacol Sci,2018,22(7):2099-2108.
[19]	Shang N, Zhou RB. Interpretation of the updates in the use of antiarrhythmic drugs during resuscitation of patients with VF/pVT cardiac arrest or immediately after ROSC in 2018 international consensus on cardiopulmonary resuscitation and emergency cardiovascular care science with treatment recommendations[J].Chin General Practice(中国全科医学),2019,22(20):2393-2397.

Cited By

Get Citation

PDF

XML

Article views (445) PDF downloads (1057)

Turn off MathJax

Article Contents

Abstract

References

Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases

Abstract

References

Catalog

Export File

Citation

Format

Content