Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases

ZHENG Binbin; ZHANG Jianan; FAN Yixin; HU Liang; LIU Wentao; WANG Xuerong

doi:10.11665/j.issn.1000-5048.20200208

Journal of China Pharmaceutical University > 2020 > 51(2): 180-184. > DOI: 10.11665/j.issn.1000-5048.20200208

ZHENG Binbin, ZHANG Jianan, FAN Yixin, HU Liang, LIU Wentao, WANG Xuerong. Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases[J]. Journal of China Pharmaceutical University, 2020, 51(2): 180-184. DOI: 10.11665/j.issn.1000-5048.20200208

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Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases

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The aim of the present study was to investigate the effects and mechanisms of lidocaine on lipopolysaccharide(LPS)-induced matrix metalloproteinase(MMP)-9 and MMP-2 activity in plasma, and the effects of lidocaine on LPS-induced acute lung injury(ALI). Mice were pretreated with lidocaine(2, 4, 8 mg/kg )for 30 minutes, and then treated with 10 mg/kg LPS(ip)for 12 h to induce ALI. The 7-day survival rate and lung wet/dry weight ratio of mice were monitored. Phosphorylation level of p38 was measured by western blot. The activity of MMP-9 and MMP-2 in plasma was evaluated by gelatin zymography. The results showed that pretreatment with lidocaine could significantly reduce the death rate of ALI mice as well as the lung wet/dry weight ratio in a dose-dependent manner and suppress the activity of MMP-9 and MMP-2 in plasma. Moreover, lidocaine also markedly inhibited LPS-induced upregulation of p-p38 in a dose-dependent manner. In conclusion, lidocaine alleviated LPS-induced acute lung injury by suppressing MMP-9 and MMP-2 activity.
- lidocaine,
- lipopolysaccharide,
- acute lung injury,
- matrix metalloproteinases

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[1]	Hung YL, Fang SH, Wang SC, et al. Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response[J].Sci Rep,2017,7:46299.
[2]	Hirano Y,Aziz M,Yang WL,et al.Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury[J].Crit Care,2015,19(1):53.
[3]	Matthay MA,Ware LB,Zimmerman GA.The acute respiratory distress syndrome[J].J Clin Invest,2012,122(8):2731-2740.
[4]	Rhee C,Dantes R,Epstein L,et al.Incidence and trends of sepsis in US hospitals using clinical vs claims data,2009-2014[J].Jama,2017,318(13):1241-1249.
[5]	Keck T,Balcom JH,Fernández-del Castillo C,et al.Matrix metalloproteinase-9 promotes neutrophil migration and alveolar capillary leakage in pancreatitis-associated lung injury in the rat[J].Gastroenterology,2002,122(1):188-201.
[6]	Sen AI, Foronjy RF, Shiomi T, et al. Loss of MMP-13 delays inflammatory response after acute hyperoxic lung injury[J].Proc Am Thorac Soc,2008,5(3):360-361.
[7]	Zhang F, Hu L, Wu YX, et al. Doxycycline alleviates paraquat-induced acute lung injury by inhibiting neutrophil-derived matrix metalloproteinase 9[J].Int Immunopharmacol,2019,72: 243-251.
[8]	Zinter MS,Delucchi KL,Kong MY,et al.Early plasma matrix metalloproteinase profiles.A novel pathway in pediatric acute respiratory distress syndrome[J].Am J Respir Crit Care Med,2019,199(2):181-189.
[9]	Chakrabarti S,Patel KD.Matrix metalloproteinase-2(MMP-2)and MMP-9 in pulmonary pathology[J].Exp Lung Res,2005,31(6):599-621.
[10]	Fan YX,Liu HJ,Li YX,et al.Quercetin ameliorates postoperative pain by suppressing matrix metalloproteinase in microglia[J].J China Pharm Univ(中国药科大学学报),2017,48(3):343-347.
[11]	Xu XL,Ji H,Gu SY,et al.Effects of astragaloside IV on experimental ventricular remodeling in mice and its mechanism from matrix metalloproteinase aspect[J].J China Pharm Univ(中国药科大学学报),2010,41(1):70-75.
[12]	Ricou B,Nicod L,Lacraz S,et al.Matrix metalloproteinases and TIMP in acute respiratory distress syndrome[J].Am J Respir Crit Care Med,1996,154(2 Pt 1):346-352.
[13]	Fujita M,Harada E,Ikegame S,et al.Doxycycline attenuated lung injury by its biological effect apart from its antimicrobial function[J].Pulm Pharmacol Ther,2007,20(6):669-675.
[14]	Carney DE, Mccann UG, Schiller HJ, et al. Metalloproteinase inhibition prevents acute respiratory distress syndrome[J].J Surg Res,2001,99(2):245-252.
[15]	Zhang Z,Zhou J,Liao C,et al.RAGE deficiency attenuates the protective effect of Lidocaine against sepsis-induced acute lung injury[J].Inflammation,2017,40(2):601-611.
[16]	Zhang H,Sha J,Feng X,et al.Dexmedetomidine ameliorates LPS induced acute lung injury via GSK-3β/STAT3-NF-κB signaling pathway in rats[J].Int Immunopharmacol,2019,74:105717.
[17]	Vassallo A,Wood AJ,Subburayalu J,et al.The counter-intuitive role of the neutrophil in the acute respiratory distress syndrome[J].Br Med Bull,2019,131(1):43-55.
[18]	Chen LJ,Ding YB,Ma PL,et al.The protective effect of lidocaine on lipopolysaccharide-induced acute lung injury in rats through NF-κB and p38 MAPK signaling pathway and excessive inflammatory responses[J].Eur Rev Med Pharmacol Sci,2018,22(7):2099-2108.
[19]	Shang N, Zhou RB. Interpretation of the updates in the use of antiarrhythmic drugs during resuscitation of patients with VF/pVT cardiac arrest or immediately after ROSC in 2018 international consensus on cardiopulmonary resuscitation and emergency cardiovascular care science with treatment recommendations[J].Chin General Practice(中国全科医学),2019,22(20):2393-2397.

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Lidocaine attenuates LPS-induced acute lung injury in mice via an inhibition on matrix metalloproteinases

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