<i>β</i>-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells

DUAN Lanlan; DONG Jing; FAN Xiangcheng; ZHU Junyi; ZHANG Yifan; HAN Jichun; SHANG Jing

doi:10.11665/j.issn.1000-5048.20200311

Journal of China Pharmaceutical University > 2020 > 51(3): 333-339. > DOI: 10.11665/j.issn.1000-5048.20200311

DUAN Lanlan, DONG Jing, FAN Xiangcheng, ZHU Junyi, ZHANG Yifan, HAN Jichun, SHANG Jing. β-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells[J]. Journal of China Pharmaceutical University, 2020, 51(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20200311

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β-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells

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Received Date: December 03, 2019
Revised Date: May 12, 2020

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Abstract

This study aimed to investigate whether β-elemene could improve the dysfunction of vascular endothelial cells induced by low shear force (LSS), and the proliferation and migration of vascular smooth muscle cells induced by oxidized low-density lipoprotein (ox-LDL). Parallel plate flow chambers and ox-LDL were used to establish vascular endothelial cells (ECs) dysfunction model and vascular smooth muscle cell (VSMCs) proliferation and migration model, respectively, and the effects of β-elemene on ECs dysfunction and VSMCs proliferation and migration were examined. The activity of ROS in ECs was measured by DHE and the activity of NO in ECs was tested by DAF-FM DA. The protein phosphorylation of Akt and ERK in ECs were detected by Western blot. The proliferation of VSMCs was measured by MTT. The migration of VSMCs was examined by cell scratch test and Transwell assay. The gene expression of MMP-2 and MMP-9 in VSMCs was measured by RT-qPCR. In ECs, β-elemene could significantly reduce the LSS-induced increase in ROS, significantly increase the LSS-induced decrease in NO, decrease the phosphorylation of ERK, and increase the phosphorylation of Akt. In VSMCs, β-elemene could significantly reduce the proliferation and migration of VSMCs induced by ox-LDL, and reduce the gene expression of MMP-2 and MMP-9. To conclude, β-elemene can improve the LSS-induced ECs dysfunction and ox-LDL-induced VSMCs proliferation and migration.
- β-elemene,
- endothelial cells dysfunction,
- vascular smooth muscle cells,
- proliferation,
- migration

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