<i>β</i>-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells

DUAN Lanlan; DONG Jing; FAN Xiangcheng; ZHU Junyi; ZHANG Yifan; HAN Jichun; SHANG Jing

doi:10.11665/j.issn.1000-5048.20200311

Journal of China Pharmaceutical University > 2020 > 51(3): 333-339. > DOI: 10.11665/j.issn.1000-5048.20200311

DUAN Lanlan, DONG Jing, FAN Xiangcheng, ZHU Junyi, ZHANG Yifan, HAN Jichun, SHANG Jing. β-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells[J]. Journal of China Pharmaceutical University, 2020, 51(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20200311

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β-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells

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Received Date: December 03, 2019
Revised Date: May 12, 2020

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Abstract

Abstract

This study aimed to investigate whether β-elemene could improve the dysfunction of vascular endothelial cells induced by low shear force (LSS), and the proliferation and migration of vascular smooth muscle cells induced by oxidized low-density lipoprotein (ox-LDL). Parallel plate flow chambers and ox-LDL were used to establish vascular endothelial cells (ECs) dysfunction model and vascular smooth muscle cell (VSMCs) proliferation and migration model, respectively, and the effects of β-elemene on ECs dysfunction and VSMCs proliferation and migration were examined. The activity of ROS in ECs was measured by DHE and the activity of NO in ECs was tested by DAF-FM DA. The protein phosphorylation of Akt and ERK in ECs were detected by Western blot. The proliferation of VSMCs was measured by MTT. The migration of VSMCs was examined by cell scratch test and Transwell assay. The gene expression of MMP-2 and MMP-9 in VSMCs was measured by RT-qPCR. In ECs, β-elemene could significantly reduce the LSS-induced increase in ROS, significantly increase the LSS-induced decrease in NO, decrease the phosphorylation of ERK, and increase the phosphorylation of Akt. In VSMCs, β-elemene could significantly reduce the proliferation and migration of VSMCs induced by ox-LDL, and reduce the gene expression of MMP-2 and MMP-9. To conclude, β-elemene can improve the LSS-induced ECs dysfunction and ox-LDL-induced VSMCs proliferation and migration.
- β-elemene,
- endothelial cells dysfunction,
- vascular smooth muscle cells,
- proliferation,
- migration

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[1]	. Circulation， 2016， 134（19）： 1430-1440.
[2]	Wu JH， Li YN， Zhou YF， et al. Semaphorin-3E attenuates neointimal formation via suppressing VSMCs migration and proliferation［J］. Cardiovasc Res， 2017， 113（14）： 1763-1775.
[3]	Rudijanto A. The role of vascular smooth muscle cells on the pathogenesis of atherosclerosis［J］. Acta Med Indones， 2007， 39（2）： 86-93.
[4]	Liu QY， Chen M. Research progress on role of shear stress in carotid atherosclerosis［J］. Oncoradiology（肿瘤影像学）， 2014， 23（1）： 68-72.
[5]	Mahmoud MM， Kim HR， Xing RY， et al. TWIST1 integrates endothelial responses to flow in vascular dysfunction and atherosclerosis［J］. Circ Res， 2016， 119（3）： 450-462.
[6]	Chao YL， Ye P， Zhu LL， et al. Low shear stress induces endothelial reactive oxygen species via the AT1R/Enos/NO pathway［J］. J Cell Physiol， 2018， 233（2）： 1384-1395.
[7]	Guo TT， Huang WP， Hu CX， et al. Effects of β-elemene on invasion and migration of human breast cancer cells［J］. Pharmacol Clin Chin Mater Med （中国药理与临床）， 2018， 34（1）： 76-80.
[8]	Jiang S， Yu G， Feng XL. Advances in antitumor status and mechanism of elemene［J］. J Liaoning Univ Tradit Chin Med（辽宁中医药大学学报）， 2018， 20（4）： 218-221.
[9]	Liu M， Chen XT， Ma J， et al. Β-Elemene attenuates atherosclerosis in apolipoprotein E-deficient mice via restoring NO levels and alleviating oxidative stress［J］. Biomedecine Pharmacother， 2017， 95： 1789-1798.
[10]	Liu M， Mao LF， Daoud A， et al. Β-elemene inhibits monocyte-endothelial cells interactions via reactive oxygen species/MAPK/NF-κB signaling pathway in vitro［J］. Eur J Pharmacol， 2015， 766： 37-45.
[11]	Kumar S， Williams D， Sur S， et al. Role of flow-sensitive microRNAs and long noncoding RNAs in vascular dysfunction and atherosclerosis［J］. Vascul Pharmacol， 2019， 114： 76-92.
[12]	Gao WH， Cui HB. Correlation between distribution of coronary lesions and low shear stress［J］. Med Recapitul （医学综述）， 2013， 19（21）： 3871-3874.
[13]	Wu LY， Wang GX， Tang ST， et al. Protection of endothelial cells， inhibition of neointimal hyperplasia by β-elemene in an injured artery［J］. Cardiovasc Drugs Ther， 2011， 25（3）： 233-242.
[14]	Schlosser A， Pilecki B， Hemstra LE， et al. MFAP4 promotes vascular smooth muscle migration， proliferation and accelerates neointima formation［J］. Arterioscler Thromb Vasc Biol， 2016， 36（1）： 122-133.
[15]	Li WF， Zhi WB， Zhao JM， et al. Cinnamaldehyde protects VSMCs against ox-LDL-induced proliferation and migration through S arrest and inhibition of p38， JNK/MAPKs and NF-κB［J］. Vascul Pharmacol， 2018， 108： 57-66.
[16]	Chen XZ， Yuan YH， Wang Q， et al. Post-translational modification of adiponectin affects lipid accumulation， proliferation and migration of vascular smooth muscle cells［J］. Cell Physiol Biochem， 2017， 43（1）： 172-181.
[17]	Li WF， Zhi WB， Liu F， et al. Atractylenolide I restores HO-1 expression and inhibits Ox-LDL-induced VSMCs proliferation， migration and inflammatory responses in vitro［J］. Exp Cell Res， 2017， 353（1）： 26-34.
[18]	Li JX， Luo W， Wang JY， et al. Influence of puerarin on endogenous NOS inhibitor of human umbilical vein endothelial cells cultured with oxidized low density lipoprotein［J］. J China Pharm Univ （中国药科大学学报）， 2004， 35（4）： 353-356.

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β-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells

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