• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
DENG Xiaoying, YANG Xuping, LIU Peifang, ZHANG Zunjian, HUANG Yin. Effect of Astragalus membranaceus on metabolic homeostasis of cardio-cerebrovascular system in mice[J]. Journal of China Pharmaceutical University, 2020, 51(4): 494-501. DOI: 10.11665/j.issn.1000-5048.20200416
Citation: DENG Xiaoying, YANG Xuping, LIU Peifang, ZHANG Zunjian, HUANG Yin. Effect of Astragalus membranaceus on metabolic homeostasis of cardio-cerebrovascular system in mice[J]. Journal of China Pharmaceutical University, 2020, 51(4): 494-501. DOI: 10.11665/j.issn.1000-5048.20200416

Effect of Astragalus membranaceus on metabolic homeostasis of cardio-cerebrovascular system in mice

Funds: This study was Foundation:supported by the Natural Science Foundation of Heilongjiang Province (No.QC2016109) and the Open Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (No.DQCP2017MS02)
More Information
  • Received Date: May 25, 2020
  • To explore the effect of Astragalus membranaceus, a traditional Chinese medicine, on metabolic homeostasis of heart, brain and blood in mice, and to elucidate the cardio-cerebrovascular protective mechanisms of AR from the perspective of metabolic regulation. Thirteen ICR male mice were randomly divided into two groups which were intragastrically administered with ultrapure water and aqueous extract of Astragalus membranaceus for 10 consecutive days, respectively. Liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) were used to comprehensively characterize the metabolic profiles of serum, heart and brain tissues. Multivariate statistical analysis combined with nonparametric tests were applied to screen and identify different metabolites, and then the related metabolic pathways were uncovered. Multivariate statistical analysis showed that the metabolic profiles of serum, heart, and brain tissues of mice after Astragalus membranaceus intervention significantly changed compared with the control group. A total of 15, 19, and 17 metabolites were identified in serum, heart, and brain tissues, respectively, among which palmitic acid and LysoPC (20∶3) were screened out from all types of biological samples. The results of metabolic pathway enrichment analysis showed that amino acid metabolism, phospholipid metabolism, fatty acid metabolism and tricarboxylic acid cycle were significantly affected. Astragalus membranaceus may protect the cardio-cerebrovascular system by regulating the metabolic homeostasis of amino acids, lipids and energy.
  • [1]
    Liu P, Zhao H, Luo Y. Anti-aging implications of Astragalus membranaceus (Huangqi): a well-known Chinese tonic [J]. Aging Dis, 2017, 8(6): 868-886.
    [2]
    Li Y, Guo S, Zhu Y, et al. Comparative analysis of twenty-five compounds in different parts of Astragalus membranaceus var. mongholicus and Astragalus membranaceus by UPLC-MS/MS [J]. J Pharm Anal, 2019, 9(6): 392-399.
    [3]
    Fu J, Wang Z, Huang L, et al. Review of the botanical characteristics, phytochemistry, and pharmacology of Astragalus membranaceus (Huangqi) [J]. Phytother Res, 2014, 28(9): 1275-1283.
    [4]
    Liu Y, Xu W, Xiong Y, et al. Evaluations of the effect of HuangQi against heart failure based on comprehensive echocardiography index and metabonomics [J]. Phytomedicine, 2018, 50(2): 5-12.
    [5]
    Dong Z, Zhao P, Xu M, et al. Astragaloside IV alleviates heart failure via activating PPARα to switch glycolysis to fatty acid beta-oxidation [J]. Sci Rep, 2017, 7(1): 2691-2699.
    [6]
    Shanzad M, Shabbir A, Wojcikowski K, et al. The antioxidant effects of Radix Astragali (Astragalus membranaceus and related species) in protecting tissues from injury and disease [J]. Curr Drug Targets, 2016, 17(12): 1331-1340.
    [7]
    Agyemang K, Han L, Liu E, et al. Recent advances in Astragalus membranaceus anti-diabetic research: pharmacological effects of its phytochemical constituents [J]. Evid Based Complement Alternat Med, 2013(65): 46-48.
    [8]
    Wang X, Wang Y, Hu JP, et al. Astragaloside IV, a natural PPARγ agonist, reduces Aβ production in Alzheimer's disease through inhibition of BACE1 [J]. Mol Neurobiol, 2017, 54(4): 2939-2949.
    [9]
    Yang Q, Zhang AH, Miao JH, et al. Metabolomics biotechnology, applications, and future trends: a systematic review [J]. RSC Advances, 2019, 9(64): 37245-37257.
    [10]
    Wu G, Zhang W, Li H. Application of metabolomics for unveiling the therapeutic role of traditional Chinese medicine in metabolic diseases [J]. J Ethnopharmacol, 2019, 242(11): 2057.
    [11]
    Wang M, Chen L, Liu D, et al. Metabolomics highlights pharmacological bioactivity and biochemical mechanism of traditional Chinese medicine [J]. Chem Biol Interact, 2017, 273(1): 33-41.
    [12]
    Han L, Wang P, Wang Y, et al. Rapid discovery of the potential toxic compounds in polygonum multiflorum by UHPLC-Q-Orbitrap-MS-based metabolomics and correlation analysis [J]. Front Pharmacol, 2019, 10(3):2-9.
    [13]
    Wang R, Yu Z, Herder C, et al. Novel biomarkers for pre-diabetes identified by metabolomics [J]. Mol Syst Biol, 2012, 8(6):15-21.
    [14]
    Frisardi V, Panza F, Seripa D, et al. Glycerophospholipids and glycerophospholipid-derived lipid mediators: a complex meshwork in Alzheimer's disease pathology [J]. Prog Lipid Res, 2011, 50(4): 313-330.
    [15]
    Lin J, Liu WT, Li W, et al. Plasma metabolic profiling of Alzheimer's disease by liquid chromatography/mass spectrometry [J]. Clin Biochem, 2010, 43(12): 992-997.
    [16]
    Wu Q, Zhang H, Dong X, et al. UPLC-Q-TOF/MS based metabolomic profiling of serum and urine of hyperlipidemic rats induced by high fat diet [J]. J Pharm Anal, 2014, 4(6): 360-367.
    [17]
    Song X, Wang J, Wang P, et al. (1)H NMR-based metabolomics approach to evaluate the effect of Xue-Fu-Zhu-Yu decoction on hyperlipidemia rats induced by high-fat diet [J]. J Pharm Biomed Anal, 2013, 78(79):202-210.
    [18]
    Liu YT, Jia HM, Chang X, et al. The metabolic disturbances of isoproterenol induced myocardial infarction in rats based on a tissue targeted metabonomics [J]. Mol Biosyst, 2013, 9(11): 2823-2834.
    [19]
    Chen T, Ni Y, Ma X, et al. Branched-chain and aromatic amino acid profiles and diabetes risk in Chinese populations [J]. Sci Rep, 2016, 6(20):59-64.
    [20]
    Ferrari F, Gornir A, Hoyer S, et al. Glutamate metabolism in cerebral mitochondria after ischemia and post-ischemic recovery during aging: relationships with brain energy metabolism [J]. J Neurochem, 2018, 146(4): 416-428.
    [21]
    Yu J, Kong L, Zhang A, et al. High-throughput metabolomics for discovering potential metabolite biomarkers and metabolic mechanism from the APPswe/PS1dE9 transgenic model of Alzheimer's disease [J]. J Proteome Res, 2017, 16(9): 3219-3228.
    [22]
    Fiandaca MS, Gross TJ, Johnosn TM, et al. Potential metabolomic linkage in blood between Parkinson's disease and traumatic brain injury [J]. Metabolites, 2018, 8(3):50-52.
    [23]
    Sasaki K. Nutrition and dopamine: an intake of tyrosine in royal jelly can affect the brain levels of dopamine in male honeybees (Apis mellifera L.) [J]. J Insect Physiol, 2016, 87(3): 45-52.
    [24]
    Yi L, Shi S, Wang Y, et al. Serum metabolic profiling reveals altered metabolic pathways in patients with post-traumatic cognitive impairments [J]. Sci Rep, 2016, 6(2): 13-20.
    [25]
    Li Y, Ju L, Hou Z, et al. Screening, verification, and optimization of biomarkers for early prediction of cardiotoxicity based on metabolomics [J]. J Proteome Res, 2015, 14(6): 2437-2445.
  • Related Articles

    [1]CHU Xuxin, BU Fanxue, YIN Tingjie, HUO Meirong. Antitumor strategies based on targeted modulation of tumor-associated macrophages[J]. Journal of China Pharmaceutical University, 2021, 52(3): 261-269. DOI: 10.11665/j.issn.1000-5048.20210301
    [2]FENG Yang, XU Xiao, MO Ran. Advances in lymphatic targeted drug delivery system for treatment of tumor metastasis[J]. Journal of China Pharmaceutical University, 2020, 51(4): 425-432. DOI: 10.11665/j.issn.1000-5048.20200406
    [3]TANG Keqin, LIN Huaqing, LI Shuhong, DONG Lixin, LU Bohong, JIANG Hong. Advances in tumor targeted nanocrystals[J]. Journal of China Pharmaceutical University, 2020, 51(4): 418-424. DOI: 10.11665/j.issn.1000-5048.20200405
    [4]SONG Ke, PAN Hao, HAN Jiayi, CHEN Lijiang. Nano drug delivery system based strategies to target tumor microenvironment[J]. Journal of China Pharmaceutical University, 2018, 49(4): 392-400. DOI: 10.11665/j.issn.1000-5048.20180402
    [5]CAI Han, LIU Yanhong, YIN Tingjie, ZHOU Jianping, HUO Meirong. Advances in the targeted therapy of tumor-associated fibroblasts[J]. Journal of China Pharmaceutical University, 2018, 49(1): 20-25. DOI: 10.11665/j.issn.1000-5048.20180103
    [6]WANG Letian, WANG Jinglin, LIU Hongwu, GE Ying, LI Yuyan, XU Qingxiang. Process in targeted contrast agents for cancer imaging[J]. Journal of China Pharmaceutical University, 2017, 48(6): 635-645. DOI: 10.11665/j.issn.1000-5048.20170602
    [7]SHANG Yunkai, JU Caoyun, XIE Daping, ZHANG Can. Preparation and characterization of tumor targeting doxorubicin liposomesmodified via click chemistry[J]. Journal of China Pharmaceutical University, 2016, 47(6): 708-713. DOI: 10.11665/j.issn.1000-5048.20160613
    [8]XU Yuhong, CAO Jing. Perspectives on targeted nano-drug carriers for tumor treatment[J]. Journal of China Pharmaceutical University, 2013, 44(6): 494-503. DOI: 10.11665/j.issn.1000-5048.20130602
    [9]SHI Yongping, SU Zhigui, XIAO Yanyu, SUN Minjie, PING Qineng, ZONG Li. Preparation and characterization of dual-targeting nanostructure lipid carriers loading mitoxantrone hydrochloride for bone tumor[J]. Journal of China Pharmaceutical University, 2013, 44(4): 321-327. DOI: 10.11665/j.issn.1000-5048.20130407
    [10]LI Sai, XIAO Yan-yu, SU Zhi-gui, SHI Lu, PING Qi-neng, ZHANG Can. Preparation of BSA-coated cationic nanostructure lipid carriers and pharmacokinetics and biodistribution after intravenous injection[J]. Journal of China Pharmaceutical University, 2012, 43(5): 406-411.

Catalog

    Article views (243) PDF downloads (342) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return