• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
CHEN Yingjie, GAO Xiangdong, CHEN Song. Effects and mechanisms of FGF21 on neuronal damage induced by rotenone[J]. Journal of China Pharmaceutical University, 2020, 51(6): 718-723. DOI: 10.11665/j.issn.1000-5048.20200611
Citation: CHEN Yingjie, GAO Xiangdong, CHEN Song. Effects and mechanisms of FGF21 on neuronal damage induced by rotenone[J]. Journal of China Pharmaceutical University, 2020, 51(6): 718-723. DOI: 10.11665/j.issn.1000-5048.20200611
shu

Effects and mechanisms of FGF21 on neuronal damage induced by rotenone

Funds: This study was supported by the National Natural Science Foundation of China (No. 81673435,No.81872850) and the
More Information
  • Received Date: May 12, 2020
  • Revised Date: September 23, 2020
    Graphical Abstract
    • Abstract
  • In this study,the SH-SY5Y cell model with Parkinson"s disease-like lesions was established by using rotenone,and the effect and mechanism of fibroblast growth factor 21 (FGF21) on the cell model were explored. Different concentrations of FGF21 were used to treat neuronal injury model induced by rotenone,and cell viabilities were detected by MTT assay. Effects of FGF21 and rotenone on the apoptotic levels of SH-SY5Y cells were analyzed by using Annexin V-FITC detection kit. Western blot was used to assess the effects of FGF21 and rotenone on the protein levels of tyrosine hydroxylase (TH) and α-synuclein (α-syn) in SH-SY5Y cells. Effects of FGF21 and rotenone on reactive oxygen species (ROS) levels in SH-SY5Y cells were tested using DCFH-DA fluorescent probe. The results showed that FGF21 could reduce the damage in SH-SY5Y cells induced by rotenone,inhibit cell apoptosis,alleviate the abnormalities of TH and α-syn in SH-SY5Y cells induced by rotenone and down-regulate the abnormal ROS levels in SH-SY5Y cells. The results suggested that FGF21 may attenuate rotenone-induced neuronal damage through regulation of oxidative stress.
    • FullText(HTML)
    • References (26)
  • [1]
    . Nat Neurosci，2000，3（12）：1301?1306.
    [2]
    Collier TJ，Kanaan NM，Kordower JH. Ageing as a primary risk factor for Parkinson''''s disease：evidence from studies of non-human primates［J］. Nat Rev Neurosci，2011，12（6）：359?366.
    [3]
    Braak H，Del Tredici K，Rüb U，et al. Staging of brain pathology related to sporPDic Parkinson''''s disease［J］. Neurobiol Aging，2003，24（2）：197?211.
    [4]
    Ristow M. Neurodegenerative disorders associated with diabetes mellitus［J］. J Mol Med，2004，82（8）：510?529.
    [5]
    Moran C，Beare R，Wang W，et al. Type 2 diabetes mellitus，brain atrophy，and cognitive decline［J］. Neurology，2019，92（8）：e823?e830.
    [6]
    Leng Y，Wang Z，Tsai LK，et al. FGF-21，a novel metabolic regulator，has a robust neuroprotective role and is dramatically elevated in neurons by mood stabilizers［J］. Mol Psychiatry，2015，20（2）：215?223.
    [7]
    Yu Y，Bai F，Wang W，et al. Fibroblast growth factor 21 protects mouse brain against D-galactose induced aging via suppression of oxidative stress response and advanced glycation end products formation［J］. Pharmacol Biochem Behav，2015，133：122?131.
    [8]
    Chen S，Chen ST，Sun Y，et al. Fibroblast growth factor 21 ameliorates neurodegeneration in rat and cellular models of Alzheimer’s disease［J］. Redox Biol，2019，22：101133.
    [9]
    Yin J，Bao LC，Tian H，et al. Genetic fusion of human FGF21 to a synthetic polypeptide improves pharmacokinetics and pharmacodynamics in a mouse model of obesity［J］. Br J Pharmacol，2016，173（14）：2208?2223.
    [10]
    Venkatesh GV，Rajasankar S，Ramkumar M，et al. Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells［J］. Nutr Neurosci，2018，21（2）：97?107.
    [11]
    Hsuchou H，Pan W，Kastin AJ. The fasting polypeptide FGF21 can enter brain from blood［J］. Peptides，2007，28（12）：2382?2386.
    [12]
    Fon Tacer K，Bookout AL，Ding X，et al. Research resource：comprehensive expression atlas of the fibroblast growth factor system in adult mouse［J］. Mol Endocrinol，2010，24（10）：2050?2064.
    [13]
    Bookout AL，De Groot MH，Owen BM，et al. FGF21 regulates metabolism and circadian behavior by acting on the nervous system［J］. Nat Med，2013，19（9）：1147?1152.
    [14]
    Sun Y，Gao XD，Chen S. Effect and mechanism of FGF21 on astrocyte damage induced by Aβ25-35［J］. J China Pharm Univ（中国药科大学学报），2019，50（4）：490?496.
    [15]
    M?Kel? J，Tselykh TV，Maiorana F，et al. Fibroblast growth factor-21 enhances mitochondrial functions and increases the activity of PGC-1α in human dopaminergic neurons via Sirtuin-1［J］. Springer Plus，2014，3（1）：2.
    [16]
    Schapira AH，Jenner P. Etiology and pathogenesis of Parkinson''''s disease［J］. Mov Disord，2011，26（6）：1049?1055.
    [17]
    Zhu J，Chu CT. Mitochondrial dysfunction in Parkinson''''s disease［J］. J Alzheimers Dis，2010，20：S325?334.
    [18]
    Jenner P，Olanow CW. The pathogenesis of cell death in Parkinson''''s disease［J］. Neurology，2006，66（10 Suppl 4）：S24?36.
    [19]
    Parker WDJr，Parks JK，Swerdlow RH. Complex I deficiency in Parkinson''''s disease frontal cortex［J］. Brain Res，2008，1189：215?218.
    [20]
    Henchcliffe C，Beal MF. Mitochondrial biology and oxidative stress in Parkinson''''s disease pathogenesis［J］. Nat Clin Pract Neurol，2008，4（11）：600?609.
    [21]
    Gandhi S，Wood NW. Molecular pathogenesis of Parkinson''''s disease［J］. Hum Mol Genet，2005，14（18）：2749?2755.
    [22]
    Sun C，Mo M，Wang Y，et al. Activation of the immunoproteasome protects SH-SY5Y cells from the toxicity of rotenone［J］. Neurotoxicology，2019，73：112?119.
    [23]
    Ramalingam M，Huh YJ，Lee YI. The impairments of α-synuclein and mechanistic target of rapamycin in rotenone-induced SH-SY5Y cells and mice model of Parkinson''''s disease ［J］. Front Neurosci，2019，13：1028.
    [24]
    Yun HM，Jin P，Park KR，et al. Thiacremonone potentiates anti-oxidant effects to improve memory dysfunction in an APP/PS1 transgenic mice model［J］. Mol Neurobiol，2016，53（4）：2409?2420.
    [25]
    Chattopadhyaya I，Gupta S，Mohammed A，et al. Neuroprotective effect of Spirulina fusiform and amantadine in the 6-OHDA induced Parkinsonism in rats［J］. BMC Complement Altern Med，2015，15：296.
    [26]
    Alghamdi BSA. Possible prophylactic anti-excitotoxic and anti-oxidant effects of virgin coconut oil on aluminium chloride- induced Alzheimer''''s in rat models［J］. J Integr Neurosci，2018，17（3/4）：593?607.
    • Related Articles

  • Related Articles

    [1]CHEN Xin, YANG Qian, YOU Qidong, GUO Xiaoke. Advances of inhibitors targeting MLL1-WDR5 protein-protein interaction[J]. Journal of China Pharmaceutical University, 2022, 53(2): 125-136. DOI: 10.11665/j.issn.1000-5048.20220201
    [2]ZHANG Yuxin, DING Ming, LIU Jun. Research progress of proximity labeling technology based on biotin ligase in proteomics[J]. Journal of China Pharmaceutical University, 2022, 53(1): 18-24. DOI: 10.11665/j.issn.1000-5048.20220103
    [3]MO Xiaofei, XU Xiaoli, WANG Yalou, YOU Qidong. Advances in the HIF-1α/p300 protein-protein interaction inhibitors[J]. Journal of China Pharmaceutical University, 2017, 48(5): 515-522. DOI: 10.11665/j.issn.1000-5048.20170502
    [4]LIU Fang, SUN Haopeng, YOU Qidong. Advances in small-molecule inhibitors targeting Hsp90-Cdc37 protein-protein interaction[J]. Journal of China Pharmaceutical University, 2015, 46(3): 272-278. DOI: 10.11665/j.issn.1000-5048.20150303
    [5]The Effect of Inducer on Enzyme Activity of Dihydropyri midinase[J]. Journal of China Pharmaceutical University, 1999, (3): 60-63.
    [6]Purification and Characterization of Potent Fibrinolytic Enzyme from Earthworm[J]. Journal of China Pharmaceutical University, 1997, (6): 48-51.
    [7]Pharmacokinetic Studies of Nevin Fibrinolytic Enzyme[J]. Journal of China Pharmaceutical University, 1997, (2): 107-110.
    [8]Effect of Astragalus on Fluidity of Lipid Regions and Conformation of Protein of Human Erythrocyte Membranes[J]. Journal of China Pharmaceutical University, 1994, (4): 49-52.
    [9]Interaction of Four Berbamine Derivatives with Erythrocyte Membrane Ca~(2 )-Mg~(2 )-ATPase[J]. Journal of China Pharmaceutical University, 1993, (5): 304-307.
    [10]Wu Wutong. Advances in enzyme drugs[J]. Journal of China Pharmaceutical University, 1982, (3): 59-64.

  • Cited by

    Periodical cited type(3)

    1. 李锦华，祁萍，乔慧. ALK-TKI耐药后获得性RET融合晚期非小细胞肺癌1例报道并文献复习. 肿瘤防治研究. 2024(08): 703-706 .
    2. 王瑞峰，赵相欣，孙印，秦桥花，赵冬梅. 降解FAK的PROTAC分子的设计合成及生物活性研究. 中国药物化学杂志. 2023(07): 481-489 .
    3. 王梓灵，郭瑜婕，朱芸芸，陈乐，吴婷，刘大会，黄必胜，杜鸿志. 金花茶有效部位抑制表皮生长因子受体(EGFR)抗非小细胞肺癌的作用机制研究. 中国中药杂志. 2021(20): 5362-5371 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views (164) PDF downloads (780) Cited by(5)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：365379

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return