Masitinib alleviated cerebral ischemia/reperfusion injury by inhibiting autophagy and apoptosis

WANG Yan; PING Fengfeng; ZHOU Danli; CHEN Yanhua; LING Jingjing

doi:10.11665/j.issn.1000-5048.20210212

Journal of China Pharmaceutical University > 2021 > 52(2): 227-235. > DOI: 10.11665/j.issn.1000-5048.20210212

WANG Yan, PING Fengfeng, ZHOU Danli, CHEN Yanhua, LING Jingjing. Masitinib alleviated cerebral ischemia/reperfusion injury by inhibiting autophagy and apoptosis[J]. Journal of China Pharmaceutical University, 2021, 52(2): 227-235. DOI: 10.11665/j.issn.1000-5048.20210212

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Masitinib alleviated cerebral ischemia/reperfusion injury by inhibiting autophagy and apoptosis

1.
Department of Pharmacy, The Affiliated Wuxi Children's Hospital of Nanjing Medical University, Wuxi 214023
2.
Department of Reproductive Medicine, Wuxi People's Hospital affiliated to Nanjing Medical University, Wuxi 214023, China

Funds: This study was supported by the Scientific Research Project of Wuxi Health Commission (No.Q202010); the Six Talent Peak Project of Jiangsu Province (No.YY-128) and Wuxi Taihu Talent Plan Top Talents Project (No.BJ2020088, No.BJ2020001)

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Received Date: September 25, 2020
Revised Date: March 24, 2021

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Abstract

Abstract

To investigate the neuroprotective effect and possible mechanism of masitinib on cerebral ischemia-reperfusion injury in rats, healthy adult male Sprague-Dawley rats were divided into sham group (n = 12), model group (n = 12), masitinib low dosage group (n = 12), masitinib middle dosage group (n = 12), and masitinib high dosage group (n = 12). All rats was subjected to middle cerebral artery occlusion (MCAO) for two hours and reperfusion except sham group, and received treatment twice per day for 7 days once reperfusion started.Neurological score, infarct volume, and brain water content were detected; some autophagic markers, apoptotic and inflammatory cytokines were evaluated by Western blot and PCR after 7 d of reperfusion. Treatment with masitinib significantly ameliorated neurologic deficit, infarct volume and brain water after I/R injury. Masitinib also decreased the ratio of LC3II/I and the expression of Beclin-1 and increased the expression of p62 in the brain tissues of rats with I/R injury.Furthermore, it could inhibit apoptosis-related proteins and NF-κB expression. Masitinib could relieve the cerebral ischemia-reperfusion injury in rats through inhibiting autophagy and apoptosis.
- masitinib,
- neuroprotection,
- cerebral ischemia/reperfusion,
- autophagy,
- NF-κB signaling pathway

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References

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Masitinib alleviated cerebral ischemia/reperfusion injury by inhibiting autophagy and apoptosis

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